复方辣木颗粒剂影响雄性小鼠性功能的实验研究
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摘要
目的观察复方辣木颗粒剂对雄性小鼠性功能的影响。方法建立醋酸铅致雄性小鼠生精障碍模型。将60只实验小鼠分为正常组、模型组、阳性对照组和复方辣木颗粒剂高、中、低剂量组,每组10只。给药后,观察小鼠体重、脏器指数、附睾精子活力、精子活率及睾丸的病理形态等。结果复方辣木颗粒剂对醋酸铅致少弱精子症小鼠体重无明显影响,各给药组小鼠附睾系数、睾丸系数、精子活力、精子活率均有所上升,生精细胞和间质细胞数量增多、排列更紧密。各剂量间比较,高剂量效果更显著(均P<0.05)。结论复方辣木颗粒剂可显著改善小鼠性功能,为临床合理应用提供有利参考。
Objective To understand and confirm the effect of Compound Moringa Granules on the sexual function of male mice. Methods Establish a model of spermatogenic disorder in male mice induced by lead acetate.The male kunming mice were divided into normal group,model group,positive control group and the groupwith high,medium and low doses of Compound Moringa Granules.After treatment,themouse body weight,organ index,epididymal sperm motility,sperm motility and pathological morphology of testis were observed. Results Compound Moringa Granuleshad had no significant effect on the body weight of mice with oligozoospermia induced by lead acetate.Compared with the model group,the epididymis coefficient,testis coefficient,sperm motility and sperm motility of the mice in each administration group increased.The number of spermatogenic cells and mesenchymal cells increased and the arrangement ofcells was more tight(P< 0.05 for all).The comparison between the doses showed that the effect of high dose group was more obvious. Conclusion Compound Moringa Granulescan significantly improve the sexual function of mice,and provide a favorable reference for the clinical rational application of the drug.
Objective To understand and confirm the effect of Compound Moringa Granules on the sexual function of male mice. Methods Establish a model of spermatogenic disorder in male mice induced by lead acetate.The male kunming mice were divided into normal group,model group,positive control group and the groupwith high,medium and low doses of Compound Moringa Granules.After treatment,themouse body weight,organ index,epididymal sperm motility,sperm motility and pathological morphology of testis were observed. Results Compound Moringa Granuleshad had no significant effect on the body weight of mice with oligozoospermia induced by lead acetate.Compared with the model group,the epididymis coefficient,testis coefficient,sperm motility and sperm motility of the mice in each administration group increased.The number of spermatogenic cells and mesenchymal cells increased and the arrangement ofcells was more tight(P< 0.05 for all).The comparison between the doses showed that the effect of high dose group was more obvious. Conclusion Compound Moringa Granulescan significantly improve the sexual function of mice,and provide a favorable reference for the clinical rational application of the drug.
引文
[1] 范红艳,李小龙,张小洁,等.苦杏仁苷对醋酸铅所致雄性小鼠生精障碍的影响[J].毒理学杂志,2016,30(5):353-357.
[2] 梁未雯,万波,王张,等.民族药蔓菁膏对小鼠性功能的影响研究[J].成都中医药大学学报,2015,38(2):30-33.
[3] 袁卓珺,汤林,马栋,等.男性不育症阳虚体质与中医证候相关性研究[J].时珍国医国药,2018,29(8):1946-1947.
[4] Xu DH,Wang LH,Mei XT,et al.Protective effects of seahorse extracts in a rat castration and testosterone-induced benign prostatic hyperplasia model and mouse oligospermatism model [J].Environ Toxicol Pharmacol,2014,37(2):679-688.
[5] Dong WW,Huang HL,Yang W,et al.Testis-specific Fank1 gene in knockdown mice produces oligospermia via apoptosis [J].Asian J Androl,2014,16(1):124-130.
[6] 周才琼,阳长敏,吴雪琴,等.食品资源引进研究——海南产印度辣木的营养价值评价[J].西南农业大学学报(自然科学版),2005,27(6):763-765.
[7] 刘子记,孙继华,刘昭华,等.特色植物辣木的应用价值及发展前景分析[J].热带作物学报,2014,35(9):1871-1878.
[8] Jayawardana BC,Liyanage R,Lalantha N,et al.Antioxidant and antimicrobial activity of drumstick (Moringa oleifera) leaves in herbal chicken sausages [J].LWT-Food Science and Technology,2015,64(2):1204-1208.
[9] 杨丽华,郎晓蓉.辣木粉主要营养成分分析研究[J].农家参谋,2018,(19):73-74.
[10] 张黎明,陈志龙.海蛇的药用价值[J].中医药学报,2002,(5):25-26.
[11] 姚海萍,梁振纲,赵李妮,等.平颏海蛇化学成分的研究Ⅱ[J].时珍国医国药,2016,27(3):599-602.
[12] 国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015.294.
[13] Shuiying L.Effect of the working environment on the male reproductive system [J].China Medicine and Pharmacy,2012,2(16):124-125.
[2] 梁未雯,万波,王张,等.民族药蔓菁膏对小鼠性功能的影响研究[J].成都中医药大学学报,2015,38(2):30-33.
[3] 袁卓珺,汤林,马栋,等.男性不育症阳虚体质与中医证候相关性研究[J].时珍国医国药,2018,29(8):1946-1947.
[4] Xu DH,Wang LH,Mei XT,et al.Protective effects of seahorse extracts in a rat castration and testosterone-induced benign prostatic hyperplasia model and mouse oligospermatism model [J].Environ Toxicol Pharmacol,2014,37(2):679-688.
[5] Dong WW,Huang HL,Yang W,et al.Testis-specific Fank1 gene in knockdown mice produces oligospermia via apoptosis [J].Asian J Androl,2014,16(1):124-130.
[6] 周才琼,阳长敏,吴雪琴,等.食品资源引进研究——海南产印度辣木的营养价值评价[J].西南农业大学学报(自然科学版),2005,27(6):763-765.
[7] 刘子记,孙继华,刘昭华,等.特色植物辣木的应用价值及发展前景分析[J].热带作物学报,2014,35(9):1871-1878.
[8] Jayawardana BC,Liyanage R,Lalantha N,et al.Antioxidant and antimicrobial activity of drumstick (Moringa oleifera) leaves in herbal chicken sausages [J].LWT-Food Science and Technology,2015,64(2):1204-1208.
[9] 杨丽华,郎晓蓉.辣木粉主要营养成分分析研究[J].农家参谋,2018,(19):73-74.
[10] 张黎明,陈志龙.海蛇的药用价值[J].中医药学报,2002,(5):25-26.
[11] 姚海萍,梁振纲,赵李妮,等.平颏海蛇化学成分的研究Ⅱ[J].时珍国医国药,2016,27(3):599-602.
[12] 国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015.294.
[13] Shuiying L.Effect of the working environment on the male reproductive system [J].China Medicine and Pharmacy,2012,2(16):124-125.