红景天苷对大鼠力竭运动性肾损伤的预防和保护作用
|
摘要
目的:探讨红景天苷对大鼠力竭运动性肾损伤的保护机制。方法:将SD大鼠随机分为对照组、模型组、红景天苷组。红景天苷组给予红景天苷,模型组和对照组给予蒸馏水,于运动后24 h检测尿中性粒细胞明胶酶载脂蛋白(NGAL),腹主动脉采血检测肾功能、N-乙酰β-D氨基葡萄糖苷酶(NAG酶)、IL-10和TNF-α变化。结果:力竭运动可引起尿NAG酶和血尿素氮和NGAL水平升高,促炎因子TNF-α水平显著上升;红景天苷可明显降低大鼠过度运动后尿NAG酶排泄,降低血尿素氮和TNF-α水平,而IL-10/TNF-α比值显著升高。结论:力竭运动可引起肾小管损伤;红景天苷对大鼠过度运动后肾损伤具有预防保护作用,其机制可能与抗氧化应激及减轻炎症反应有关。
Objective: To investigate protective mechanism of salidroside on exhaustive exercise-induced renal injury in rats.Methods: All rats were divided into the control group, the model group and the salidroside group. The salidroside group was given salidroside; and the control group and the model group were given distilled water. After 24 hours of exercise, urine neutrophil gelatinase apolipoprotein(NGAL), abdominal aorta blood sampling for renal function, N-acetyl β-D glucosaminidase(NAG enzyme), IL-10 and TNF-α were detected. Results: Urine NAG enzymes, blood urea nitrogen, NGAL levels and TNF-α levels are elevated due to exhaustive exercise. Salidroside can significantly reduce urinary NAG enzyme excretion after excessive exercise in rats, reduce blood urea nitrogen and TNF-α levels, and increase IL-10/TNF-α ratio. Conclusion: Exhaustive exercise can cause tubular damage. Salidroside can prevent renal injury after excessive exercise, and its mechanism may be related to antioxidative stress and reduction of inflammatory response.
Objective: To investigate protective mechanism of salidroside on exhaustive exercise-induced renal injury in rats.Methods: All rats were divided into the control group, the model group and the salidroside group. The salidroside group was given salidroside; and the control group and the model group were given distilled water. After 24 hours of exercise, urine neutrophil gelatinase apolipoprotein(NGAL), abdominal aorta blood sampling for renal function, N-acetyl β-D glucosaminidase(NAG enzyme), IL-10 and TNF-α were detected. Results: Urine NAG enzymes, blood urea nitrogen, NGAL levels and TNF-α levels are elevated due to exhaustive exercise. Salidroside can significantly reduce urinary NAG enzyme excretion after excessive exercise in rats, reduce blood urea nitrogen and TNF-α levels, and increase IL-10/TNF-α ratio. Conclusion: Exhaustive exercise can cause tubular damage. Salidroside can prevent renal injury after excessive exercise, and its mechanism may be related to antioxidative stress and reduction of inflammatory response.
引文
[1]李猛,周春华,余永武,等.高强度军事训练致非创伤性肾损伤相关因素分析[J].军医进修学院学报,2009,30(5):635-636
[2]陈洪,周春华.高强度军事训练致急性肾损伤动物模型的研究[J].解放军医学杂志,2007,32(12):1304-1306.
[3]郭林,曹建民,徐晓阳,等.力竭运动导致大鼠急性肾小管损伤机制的研究[J].中国运动医学杂志,1999,18(2):129-130.
[4]Yusof A,Leithauser MR,Roth HJ,et al.Exercise-induced he-molysis is caused by protein modification and most evident during the earlyphase of an ultraendurance race[J].J Appl Physiol,2007,102(2):582-586.
[5]Mehryar Habibi,Yoshikazu TB,et al.Oxidative stress induced lipocalin 2 gene expression:addressing its expression under the harmful condition[J].J Radiat Res,2007,48(1):39-44.
[6]Soni SS,Ronco C,Katz N,et al.Early diagnosis of acute kidney injury:the promise of novel biomarkers[J].Blood Purif,2009,28(1):165-174.
[7]练艺影.不同剂量健步走运动对绝经后女性低度慢性炎症因子的影响[D].北京:北京体育大学,2013.
[2]陈洪,周春华.高强度军事训练致急性肾损伤动物模型的研究[J].解放军医学杂志,2007,32(12):1304-1306.
[3]郭林,曹建民,徐晓阳,等.力竭运动导致大鼠急性肾小管损伤机制的研究[J].中国运动医学杂志,1999,18(2):129-130.
[4]Yusof A,Leithauser MR,Roth HJ,et al.Exercise-induced he-molysis is caused by protein modification and most evident during the earlyphase of an ultraendurance race[J].J Appl Physiol,2007,102(2):582-586.
[5]Mehryar Habibi,Yoshikazu TB,et al.Oxidative stress induced lipocalin 2 gene expression:addressing its expression under the harmful condition[J].J Radiat Res,2007,48(1):39-44.
[6]Soni SS,Ronco C,Katz N,et al.Early diagnosis of acute kidney injury:the promise of novel biomarkers[J].Blood Purif,2009,28(1):165-174.
[7]练艺影.不同剂量健步走运动对绝经后女性低度慢性炎症因子的影响[D].北京:北京体育大学,2013.