摘要
目的:应用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法测定黄连清胃丸中栀子苷、知母皂苷AⅢ和知母皂苷BⅡ的含量。方法:采用C18色谱柱(4.6 mm×250 mm,5μm);流速为0.9 ml/min;乙腈-水(30∶70)为流动相;ELSD漂移管温度为90℃;载气(N2)流速为2.9 SLPM/min。结果:栀子苷、知母皂苷AⅢ和知母皂苷BⅡ进样量分别在0.061 8~1.236 0μg(r=0.999 2)、0.047 6~0.952 0μg(r=0.999 7)和0.184 2~3.684 0μg(r=0.999 3)时进样量的自然对数值与峰面积的自然对数值呈良好的线性关系,平均加样回收率分别为97.67%、98.18%和98.36%,RSD分别为1.09%、1.01%和0.98%(n=6)。结论:本方法测定结果准确、灵敏、重复性好。
OBJECTIVE: To develop HPLC-ELSD method for determination of the contents of Geniposide, Timosaponin AⅢ and Timosaponin BⅡ in Huanglian Qingwei Pills.METHODS: C 18 column( 4.6 mm × 250 mm, 5 μm) was used as the chromatographic column with mobile phase consisting of Acetonitrile-Water( 30∶ 70) at flow rate of 0.9 ml / min; the temperature of drift tube was set at 90 ℃ and the flow rate of the carrier gas( N 2) was set at 2.9 SLPM / min.RESULTS: The linear ranges of Geniposide,Timosaponin A Ⅲ,Timosaponin B Ⅱ were 0.061 8-1.236 0 μg( r = 0.999 2),0.047 6-0.952 0 μg( r = 0.999 7) and 0.184 2-3.684 0 μg( r = 0.999 3),respectively with average recovery rates of 97.67%( RSD = 1.09%),98.18%( RSD = 1.01%) and 98.3%( RSD = 0.98%), respectively.CONCLUSION: The established method is accurate in determination results,sensitive and reproducible.
引文
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