参茸酒的食用安全性研究
|
摘要
通过动物试验评价参茸酒的食用安全性。依据《保健食品检验与评价技术规范》(2003年版)相关方法对参茸酒进行大、小鼠急性经口毒性试验、3项遗传毒性试验(Ames试验、小鼠骨髓嗜多染红细胞微核试验、小鼠精子畸形试验)、大鼠30 d喂养试验。试验结果显示,大鼠、小鼠急性经口MTD值>15000 mg/kg·BW,属无毒级;3项遗传毒性试验未见参茸酒致突变作用;30 d喂养试验,3个剂量组雌、雄大鼠的每周体重、每周进食量、每周食物利用率、总食物利用率、脏体比值、血液学指标和末期血生化指标与15%vol基酒对照组比较,雌鼠中剂量组单核细胞、雄鼠3个剂量组总蛋白显著升高(P<0.01,P<0.01,P<0.01,P<0.05),其他指标均无显著性差异(P>0.05);除大鼠自发病变外未引起脏器组织中毒性损伤。参茸酒在本试验条件下未显示有急性毒性、遗传毒性或亚急性毒性。
The edible safety of ginseng-antler liquor was assessed by animal test.The acute oral toxicity test of mice and rats,genetic toxicity tests(including mice bone marrow cell micronucleus test,mice sperm abnormality test and Ames test) and 30-day feeding test on rats were carried out in accordance with"Technical Standards for Testing & Assessment of Health Food(2003)".The results showed that,MTD for mice and rats was more than 15000 mg/kg·BW,indicating to non-toxic grade;the negative results were obtained in genetic toxicity tests;in 30-day feeding test,there was no significant difference in weight increase,food intake,food utilization,organ coefficient,blood routine examination or blood biochemistry index between treatment group and 15 %vol base liquor control group(P>0.05),except the significant increase in monocyte of middle-dose female rats and in total protein of 3 male rats treatment groups(P<0.01,P<0.01,P<0.01,P<0.05);some of the rats had pathologic changes but no toxic injury on organs was found.Accordingly,ginseng-antler liquor presented no acute toxicity,no genetic toxicity and no subchronic toxicity in the test.
The edible safety of ginseng-antler liquor was assessed by animal test.The acute oral toxicity test of mice and rats,genetic toxicity tests(including mice bone marrow cell micronucleus test,mice sperm abnormality test and Ames test) and 30-day feeding test on rats were carried out in accordance with"Technical Standards for Testing & Assessment of Health Food(2003)".The results showed that,MTD for mice and rats was more than 15000 mg/kg·BW,indicating to non-toxic grade;the negative results were obtained in genetic toxicity tests;in 30-day feeding test,there was no significant difference in weight increase,food intake,food utilization,organ coefficient,blood routine examination or blood biochemistry index between treatment group and 15 %vol base liquor control group(P>0.05),except the significant increase in monocyte of middle-dose female rats and in total protein of 3 male rats treatment groups(P<0.01,P<0.01,P<0.01,P<0.05);some of the rats had pathologic changes but no toxic injury on organs was found.Accordingly,ginseng-antler liquor presented no acute toxicity,no genetic toxicity and no subchronic toxicity in the test.
引文
[1]王楠,高晓霞,代子彦,等.鹿茸药效物质基础、药理作用、临床应用及质量控制的研究进展[J].中草药,2017,48(22):4784-4790.
[2]尹冬冬.鹿血粉鉴别方法研究[J].现代畜牧科技,2016(11):10.
[3]宋齐.人参化学成分和药理作用研究进展[J].人参研究,2017(2):47-54.
[4]张小梅.黄芪多糖的免疫调节作用及抗肿瘤作用研究进展[J].大连大学学报,2003,24(6):101-104.
[5]冯美玲.枸杞子和栀子的化学成分及其生物活性研究[D].杭州:浙江工业大学,2013.
[6]周园,董秋菊,冯薇,等.一测多评法测定南方菟丝子中7种活性成分的含量[J].中草药,2018,49(1):227-232.
[7]中华人民共和国卫生部.保健食品检验与评价技术规范(2003年版)[M].北京:中华人民共和国卫生部,2003.
[8]林嘉楠,杨擎,刘英娜,等.鹿茸多肽对氢溴酸东莨菪碱致小鼠学习记忆功能障碍的改善作用研究[J].人参研究,2017(6):21-24.
[9]袁相恋,薄士儒,李庆杰,等.鹿血化学成分和药理作用及其应用研究进展[J].经济动物学报,2011,15(4):207-211.
[10]姚梦杰,吕金朋,张乔,等.人参化学成分及药理作用研究[J].吉林中医药,2017,37(12):1261-1263.
[11]何珊,彭维兵,葛雨,等.黄芪人参配伍应用的研究进展[J].山东化工,2017,46(21):79-80.
[2]尹冬冬.鹿血粉鉴别方法研究[J].现代畜牧科技,2016(11):10.
[3]宋齐.人参化学成分和药理作用研究进展[J].人参研究,2017(2):47-54.
[4]张小梅.黄芪多糖的免疫调节作用及抗肿瘤作用研究进展[J].大连大学学报,2003,24(6):101-104.
[5]冯美玲.枸杞子和栀子的化学成分及其生物活性研究[D].杭州:浙江工业大学,2013.
[6]周园,董秋菊,冯薇,等.一测多评法测定南方菟丝子中7种活性成分的含量[J].中草药,2018,49(1):227-232.
[7]中华人民共和国卫生部.保健食品检验与评价技术规范(2003年版)[M].北京:中华人民共和国卫生部,2003.
[8]林嘉楠,杨擎,刘英娜,等.鹿茸多肽对氢溴酸东莨菪碱致小鼠学习记忆功能障碍的改善作用研究[J].人参研究,2017(6):21-24.
[9]袁相恋,薄士儒,李庆杰,等.鹿血化学成分和药理作用及其应用研究进展[J].经济动物学报,2011,15(4):207-211.
[10]姚梦杰,吕金朋,张乔,等.人参化学成分及药理作用研究[J].吉林中医药,2017,37(12):1261-1263.
[11]何珊,彭维兵,葛雨,等.黄芪人参配伍应用的研究进展[J].山东化工,2017,46(21):79-80.