15-LOX-1在炎症性肠病患者外周血和组织中的表达及其临床意义
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摘要
目的:研究15-脂氧合酶-1(15-lipoxygenase-1,15-LOX-1)在健康体检者及炎症性肠病患者外周血及组织中的表达情况,探讨15-LOX-1的临床意义。方法:采用ELISA检测40例炎症性肠病患者和40例健康体检者外周血的15-LOX-1、IL-6以及IL-10的表达,应用免疫组化方法测定炎症性肠病患者和健康体检者结肠组织中的15-LOX-1的表达。结果及结论:炎症性肠病患者的外周血中15-LOX-1及IL-6的表达水平明显高于健康体检者(P<0.01);炎症性肠病患者的外周血中IL-10的表达水平则明显低于健康体检者(P<0.01);炎症性肠病患者的结肠组织中15-LOX-1的表达呈强阳性,阳性强度明显高于健康体检者。外周血及肠道组织中15-LOX-1的检测可以作为临床炎症性肠病的辅助诊断指标。
Objective: To study the expression and clinical significance of 15-lipoxygenase-1(15-LOX-1) in serum and intestine tissues of healthy people and patients with inflammatory bowel disease. Methods:The expression of 15-LOX-1, IL-6 and IL-10 in serum from 40 patients with inflammatory bowel disease and 40 healthy people were measured by ELISA. The expression levels of 15-LOX-1 in intestine tissues from 9 patients with inflammatory bowel disease and 9 healthy people were measured by immunological histological chemistry(IHC). Results & Conclusion: The expression levels of serum 15-LOX-1 and IL-6 in the patients with inflammatory bowel disease were significantly higher than those in the healthy people(P<0.01), while the expression levels of serum IL-10 in the patients with inflammatory bowel disease were significantly lower than those in the healthy people(P <0.01). The expression of 15-LOX-1 were strong positive in intestine tissues of the patients with inflammatory bowel disease, which were weakly positive in those of the healthy people. Analysis of 15-LOX-1 in peripheral blood and intestine tissues can be used as an index of clinical auxiliary diagnosis of inflammatory bowel disease.
Objective: To study the expression and clinical significance of 15-lipoxygenase-1(15-LOX-1) in serum and intestine tissues of healthy people and patients with inflammatory bowel disease. Methods:The expression of 15-LOX-1, IL-6 and IL-10 in serum from 40 patients with inflammatory bowel disease and 40 healthy people were measured by ELISA. The expression levels of 15-LOX-1 in intestine tissues from 9 patients with inflammatory bowel disease and 9 healthy people were measured by immunological histological chemistry(IHC). Results & Conclusion: The expression levels of serum 15-LOX-1 and IL-6 in the patients with inflammatory bowel disease were significantly higher than those in the healthy people(P<0.01), while the expression levels of serum IL-10 in the patients with inflammatory bowel disease were significantly lower than those in the healthy people(P <0.01). The expression of 15-LOX-1 were strong positive in intestine tissues of the patients with inflammatory bowel disease, which were weakly positive in those of the healthy people. Analysis of 15-LOX-1 in peripheral blood and intestine tissues can be used as an index of clinical auxiliary diagnosis of inflammatory bowel disease.
引文
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[14]Liu C,Xu D,Liu L,et al.15-Lipoxygenase-1 induces expression and release of chemokines in cultured human lung epithelial cells[J].Am J Physiol Lung Cell Mol Physiol,2009,297(1):196-203.
[2]Pidgeon GP,Lysaght J,Krishnamoorthy S,et al.Lipoxygenase metabolism:roles in tumor progression and survival[J].Cancer Metastasis Rev,2007,26(3-4):503-24.
[3]Grechkin A.Recent developments in biochemistry of the plant lipoxygenase pathway[J].Prog Lipid Res,1998,37(5):317-52.
[4]Moore EE,Claude H,Organ Jr.Memorial lecture:splanchnic hypoperfusion provokes acute lung injury via a 5-lipoxygenase-dependent mechanism[J].Am J Surg,2010,200(6):681-9.
[5]Spanbroek R,Grabner R,Lotzer K,et al.Expanding expression of the 5-lipoxygenase pathway within the arterial wall during human atherogenesis[J].Proc Natl Acad Sci USA,2003,100(3):1238-43.
[6]Kelavkar UP,Eling T,Badr K.15-lipoxygenase-1 over expression in prostate adenocarcinoma[J].Adv Exp Med Biol,2002,507(507):133-45.
[7]Kelavkar UP,Cohen C.15-lipoxygenase-1 expression upregulates and activates insulin-like growth factor-1receptor in prostate cancer cells[J].Neoplasia,2004,6(1):41-52.
[8]His LC,Wilson LC,Eling TE.Opposing effects of 15-lipoxygenase-1 and-2 metabolites on MAPK signaling in prostate.Alteration inperoxisome proliferator-activated receptorgamma[J].J Biol Chem,2002,277(43):40549-56.
[9]Pidgeon GP,Lysaght J.Krishnamoorthy S,et al.Lipoxygenase metabolism:roles in tumor progression and survival[J].Cancer Metastasis Rev,2007,26(3/4):503-24.
[10]Bhattacharya S,Mathew G,Jayne DG,et al.15-Lipoxygenase-1in colorectal cancer:a review[J].Tumor Biol,2009,30(4):185-99.
[11]Claesson HE.On the biosynthesis and biological role of eoxins and 15-lipoxygenase-1 in airway inflammation and Hodgkin lymphoma[J].Prostaglandins Other Lipid Mediat.2009,89(3-4):120-125.
[12]Zhao J,Maskrey B,Balzar S,et al.Interleukin-13induced MUC5AC is regulated by 15-lipoxygenase-1pathway in human bronchial epithelial cells[J].Am JRespir Crit Care Med,2009,179(9):782-90.
[13]Hajek AR,Lindley AR,Favoreto S Jr,et al.12/15-Lipoxygenase deficiency protects mice from allergic airways inflammation and increases secretory IgA levels[J].J Allergy Clin Immunol,2008,122(3):633-9.
[14]Liu C,Xu D,Liu L,et al.15-Lipoxygenase-1 induces expression and release of chemokines in cultured human lung epithelial cells[J].Am J Physiol Lung Cell Mol Physiol,2009,297(1):196-203.