一株烟叶内源菌的鉴定及其在烟草提取物中的应用
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摘要
利用从醇化红大烟叶表面分离的产蛋白酶及多糖酶的菌株RSCT26,通过形态学及ITS区同源性序列分析,将该菌株鉴定为链格孢属(Alternaria alternata)。对菌株RSCT26进行发酵,利用分光光度法测定蛋白酶及多糖酶酶活,并分离制成RSCT26菌剂。结果发现,RSCT26菌剂处理后烟膏中可溶性总糖、还原糖含量增加,总氮及蛋白质含量降低,致香成分总量显著增加。感官评价结果表明,RSCT26菌剂处理后再造烟叶产品杂气降低,刺激变小,香气质明显改善,香气量显著提升。
RSCT26 strain producing protease and polysaccharidase was separated from the surface of alcoholized Hongda tobacco leaves. Through morphological method and ITS region homology sequence analysis, RSCT26 strain was identified as Alternaria alternata. RSCT26 strain was fermented and its protease and polysaccharidase activities were determined to produce RSCT26 preparation. The results indicated that the total amount of soluble sugar and reduced sugar content increased after treatment, and the contents of total nitrogen and protein decreased after treatment, and the total amount of aroma components significantly increased after treatment. The results of sensory evaluation showed that the offensive odor and irritancy of reconstituted tobacco after RSCT26 treatment reduced,aroma quality was obviously improved and aroma quantity obviously increased after RSCT26 treatment.
RSCT26 strain producing protease and polysaccharidase was separated from the surface of alcoholized Hongda tobacco leaves. Through morphological method and ITS region homology sequence analysis, RSCT26 strain was identified as Alternaria alternata. RSCT26 strain was fermented and its protease and polysaccharidase activities were determined to produce RSCT26 preparation. The results indicated that the total amount of soluble sugar and reduced sugar content increased after treatment, and the contents of total nitrogen and protein decreased after treatment, and the total amount of aroma components significantly increased after treatment. The results of sensory evaluation showed that the offensive odor and irritancy of reconstituted tobacco after RSCT26 treatment reduced,aroma quality was obviously improved and aroma quantity obviously increased after RSCT26 treatment.
引文
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[2] 汪华文.造纸法薄片在卷烟中的应用效果分析[J].烟草科技,2000(8):15-16.
[3] 钟德义,钟科军,李和平.复合造纸法再造烟叶的制备及效果分析[J].烟草科技,2009(7):12-14.
[4] 毛耀,何文,姚元军,等.美拉德反应在烟草薄片减害中的应用[J].氨基酸和生物资源,2012,34(1):48-50.
[5] 白晓莉,龚荣刚,彭国岗,等.再造烟叶对卷烟主流烟气中苯酚的影响[J].食品工业,2009(6):22-23.
[6] 郑小嘎,赵昌政,韦绪伦,等.酶法改善造纸法烟草薄片品质初探[J].山东食品发酵,2010(1):11-13.
[7] 王豹祥.陈化烤烟叶面优势菌种的筛选定位与增香效应研究[D].郑州:河南农业大学,2006.
[8] 郑小嘎.利用真菌处理烟叶诱香增质的研究[D].泰安:山东农业大学,2003.
[9] 周瑾,李雪梅,许传坤,等.利用微生物发酵改良烤烟碎片品质的研究[J].烟草科技,2002(6):3-5.
[10] 赵敏,汪长国,刘劲,等.微生物制剂MP喷施烟叶后的差异蛋白分析[J].烟草科技,2012(7):72-75.
[11] 汪长国,戴亚,朱立军,等.采收前叶面喷施微生物制剂改善烟叶品质试验[J].烟草科技,2006(4):31-34.
[12] 陈兴,申晓峰,巩效伟,等.利用微生物制剂提高梗丝品质的研究[J].中国烟草学报,2013,19(3):83-86.
[13] 李宁,汪长国,曾代龙,等.蜡样芽孢杆菌(Bacillus cereus)筛选鉴定及在雪茄烟叶发酵中的应用研究[J].中国烟草学报,2012,18(2):65-69.
[14] 祝明亮.酵化烟叶酵母菌分离鉴定及其生物学特性研究[J].工业微生物,2006,36(2):22-25.
[15] 魏景超.真菌鉴定手册[M].上海:上海科学技术出版社,1979.
[16] 戴芳澜.真菌的形态和分类[M].北京:科学出版社,1987.
[17] AUSUBEL F M,BRENT R,KINGSTON R E,等.精编分子生物学实验指南[M].颜子颍,王海林,译.北京:科学出版社,2001:36-39.
[18] 史宏志,刘国顺,杨惠娟,等.烟草香味学[M].北京:中国农业出版社,1998.
[19] 谢剑平,赵明月,吴鸣,等.白肋烟重要香味物质组成的研究[J].烟草科技,2002(10):3-16.
[20] 王瑞新.烟草化学[M].北京:中国农业出版社,2003.
[21] 郑勤安.造纸法再造烟叶生产过程中微生物增质剂的应用研究[J].浙江工业大学学报,2004,32(4):442-446.
[22] 闫克玉.烟草化学[M].郑州:郑州大学出版社,2002.