钩藤植物不同药用部位中钩藤碱的含量分析
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摘要
目的分析广西不同产地钩藤植物不同药用部位钩藤碱的含量。方法采用HPLC法分别测定10批钩藤药材中带钩茎枝、无茎枝钩、无钩茎枝、主杆和叶的钩藤碱含量。采用Gemini C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(含2 mmol.L-1三乙胺,冰乙酸调pH7.5)(64∶36),流速1.0 mL.min-1,检测波长254 nm。结果广西10个产地钩藤植物各个不同部位大部分可检出钩藤碱成分,钩藤碱的含量主杆>带钩茎枝、无茎枝钩、无钩茎枝>叶。结论钩藤植物不同药用部位大部分含钩藤碱成分,这为扩展钩藤植物的药用部位、充分利用钩藤资源提供了试验依据。
OBJECTIVE To quantitatively analyze the rhynchophylline in different medicinal parts of Uncaria rhynchophylla from different habitats in Guangxi,provide experimental basis for great use of Uncaria rhynchophylla from different habitats in Guangxi,and provide experimental basis for great use of Uncaria rhynchophylla plant resouces.METHODS HPLC method was performed to determine the amount of rhynchophylline in stem with hooks,hooks without stem,stem without hooks,stem and leaf of Uncaria rhynchophylla from 10 batches.Gemini C18column(250 mm×4.6 mm,5 μm)was used with methanol-water with contain 2 mmol· L-1 of triethylamine,a pH of 7.5(64:36),a flow rate of 1.0 mL·L-1 as the mobile phase and the detection wavelength was 254 nm.RESULTS Rhynchophylline could be detected in most of the medicinal parts from Uncaria rhynchophylla of 10 batches,and the amount of rhynchophylline was:stem>stem with hooks,hooks without stem,stem without hooks>leaf.CONCLUSION Most of the medicinal parts form Uncaria rhynchophylla contains rhynchophylline,which make the medicinal parts of Uncaria rhynchophylla was extended,and provide experimental basis for full use of Uncaria rhynchophylla plant resouces.
OBJECTIVE To quantitatively analyze the rhynchophylline in different medicinal parts of Uncaria rhynchophylla from different habitats in Guangxi,provide experimental basis for great use of Uncaria rhynchophylla from different habitats in Guangxi,and provide experimental basis for great use of Uncaria rhynchophylla plant resouces.METHODS HPLC method was performed to determine the amount of rhynchophylline in stem with hooks,hooks without stem,stem without hooks,stem and leaf of Uncaria rhynchophylla from 10 batches.Gemini C18column(250 mm×4.6 mm,5 μm)was used with methanol-water with contain 2 mmol· L-1 of triethylamine,a pH of 7.5(64:36),a flow rate of 1.0 mL·L-1 as the mobile phase and the detection wavelength was 254 nm.RESULTS Rhynchophylline could be detected in most of the medicinal parts from Uncaria rhynchophylla of 10 batches,and the amount of rhynchophylline was:stem>stem with hooks,hooks without stem,stem without hooks>leaf.CONCLUSION Most of the medicinal parts form Uncaria rhynchophylla contains rhynchophylline,which make the medicinal parts of Uncaria rhynchophylla was extended,and provide experimental basis for full use of Uncaria rhynchophylla plant resouces.
引文
[1]中华人民共和国国家药典委员会.中国药典[S].北京:中国医药科技出版社,2010:240.
[2]阮俊,黄永林.广西钩藤属植物中总生物碱含量变化研究[J].广西植物,2004,24(2):158-160.
[3]黄瑞松,张鹏,覃冬杰,等.大叶钩藤植物不同部位中钩藤碱含量分析[J].中成药,2012,34(4):710-713.
[4]黄瑞松,张鹏,覃冬杰,等.无柄果钩藤植物不同部位钩藤碱含量分析[J].时珍国医国药,2012,23(7):1701-1702.
[5]张鹏,覃冬杰,黄瑞松,等.HPLC测定广西产钩藤药材中的钩藤碱[J].华西药学杂志,2012,27(2):209-211.
[2]阮俊,黄永林.广西钩藤属植物中总生物碱含量变化研究[J].广西植物,2004,24(2):158-160.
[3]黄瑞松,张鹏,覃冬杰,等.大叶钩藤植物不同部位中钩藤碱含量分析[J].中成药,2012,34(4):710-713.
[4]黄瑞松,张鹏,覃冬杰,等.无柄果钩藤植物不同部位钩藤碱含量分析[J].时珍国医国药,2012,23(7):1701-1702.
[5]张鹏,覃冬杰,黄瑞松,等.HPLC测定广西产钩藤药材中的钩藤碱[J].华西药学杂志,2012,27(2):209-211.