浸矿菌的选育及对低品位钼矿的浸出试验研究
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摘要
针对有研究认为钼对细菌等微生物的生命活动具有较强的毒害和抑制作用问题,以从陕西洛南钼矿区矿坑水中分离得到自然混合菌种为对象,在驯化培养的基础上,对本矿区低品位钼矿石进行了浸钼试验。结果表明,在浸矿矿浆浓度为15 g/L、初始p H=2.0、接种量为12%、Fe2+浓度为5.5 g/L、Fe3+浓度为3.5 g/L、培养箱转速为160 r/min、浸出温度为30℃、培养时间为25 d情况下,钼的浸出率达73.57%,明显高于相应无菌化学浸出12.52%的钼浸出率。该原生混合菌群有较好浸钼效率的原因表现为2方面:其一,该原生混合菌对矿浆环境的耐受性较高;其二,该工艺过程充分激活了微生物浸矿的直接作用和间接作用过程。
Based on the studies that molybdenum has a strong toxicity and inhibitory effect on the life activities of microorganisms such as bacteria, the natural mixed strains isolated from the water of Luonan molybdenum mine in Shaanxi Province were cultivated and domesticated to leach the local low-grade molybdenum ore. The leaching conditions determined by single mineral tests were pulp concentration of 15 g/L, initial p H value of 2.0, inoculation size of 12%, Fe2+concentration of 5.5 g/L, Fe3+concentration of 3.5 g/L, incubator speed is 160 r/min, temperature of 30 ℃ and incubation time of 25 d. Conducted on this conditions, the molybdenum bioleaching rate of test was reached 73.57%, which was obviously higher than the molybdenum leaching rate only 12.52% in the corresponding sterile chemical leaching experiment. Reasons mainly come from two aspects by analyzing the experimental processes of the native mixed bacteria leaching low-grade molybdenum ore, one is that the native mixed bacteria are more tolerant to the pulp environment, and the other is that the process fully activates the synergistic effect of direct and indirect action of biological metallurgy.
Based on the studies that molybdenum has a strong toxicity and inhibitory effect on the life activities of microorganisms such as bacteria, the natural mixed strains isolated from the water of Luonan molybdenum mine in Shaanxi Province were cultivated and domesticated to leach the local low-grade molybdenum ore. The leaching conditions determined by single mineral tests were pulp concentration of 15 g/L, initial p H value of 2.0, inoculation size of 12%, Fe2+concentration of 5.5 g/L, Fe3+concentration of 3.5 g/L, incubator speed is 160 r/min, temperature of 30 ℃ and incubation time of 25 d. Conducted on this conditions, the molybdenum bioleaching rate of test was reached 73.57%, which was obviously higher than the molybdenum leaching rate only 12.52% in the corresponding sterile chemical leaching experiment. Reasons mainly come from two aspects by analyzing the experimental processes of the native mixed bacteria leaching low-grade molybdenum ore, one is that the native mixed bacteria are more tolerant to the pulp environment, and the other is that the process fully activates the synergistic effect of direct and indirect action of biological metallurgy.
引文
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[2]卢涛,魏德洲,沈岩柏,等.胶硫钼矿型难选钼矿的微生物浸出试验研究[J].中国矿业大学学报,2016(1):157-162.Lu Tao,Wei Dezhou,Shen Yanbai,et al.Bioleaching experiment of refractory Mo ore-containing jordisite[J].Journal of China University of Mining and Technology,2016(1):157-162.
[3]赵欢,张广积,杨巧文,等.低品位钼尾矿生物浸出实验研究[J].稀有金属与硬质合金,2015(6):7-11.Zhao Huan,Zhang Guangji,Yang Qiaowen,et al.Experimental study on bioleaching of low-grade molybdenum tailings[J].Rare Metalsand Cemented Carbides,2015(6):7-11.
[4]吉兆宁,余斌,刘坚,等.金堆城低品位钼矿石可浸性研究[J].有色金属:矿山部分,2002(5):15-18.Ji Zhaoning,Yu Bin,Liu Jian,et al.Study on immersion of low grade molybdenum ore in Jinduicheng[J].Nonferrous Metals:Mine Section,2002(5):15-18.
[5]张鸿彪,牟文宁,宋佩,等.钼矿冶炼新工艺发展概论[J].材料导报,2013(22):87-90.Zhang Hongbiao,Mou Wenning,Song Pei,et al.General overview on development of molybdenum ores smelting technology[J].Materials Review,2013(22):87-90.
[6]黄卉,陈福亮,姜艳,等.我国钼资源现状及钼的冶炼分析[J].云南冶金,2014(2):66-70.Huang Hui,Chen Fuliang,Jiang Yan,et al.The current situation of domestic molybdenum resources and molybdenum smelting analysis[J].Yunnan Metallurgy,2014(2):66-70.
[7]罗嵩.从辉钼矿尾矿中回收铝的实验研究[D].沈阳:东北大学,2008.Luo Song.Study on Recycling Molybdenum from Minerals Tailings[D].Shenyang:Northeastern University,2008.
[8]陈家武.嗜热金属硫叶菌浸出碳质镍钼矿的研究[D].长沙:中南大学,2012.Chen Jiawu.Study on Leaching of Nickel-molybdenum Sulfide Ore with Thermophilic Sulfolobus Metallicus[D].Changsha:Central South University,2012.
[9]周根茂,曾毅君,孟舒.用碱法从低品位难选辉钼矿中浸出钼的试验研究[J].湿法冶金,2015(6):466-469.Zhou Genmao,Zeng Yijun,Meng Shu.Leaching of molybdenum from a low grade molybdenite ore[J].Hydrometallurgy of China,2015(6):466-469.
[10]刘海英,李崇德.某低品位钼矿浮选工艺研究[J].有色金属:选矿部分,2013(1):35-39.Liu Haiying,Li Chongde.Research on floatation technology of a low grade molybdenum ore[J].Nonferrous Metals:Mineral Processing Section,2013(1):35-39.
[11]邹平,周兴龙,张文彬,等.低品位硫化铜矿生物柱浸过程细菌种群结构及演替规律[J].过程工程学报,2009(1):88-94.Zou Ping,Zhou Xinglong,Zhang Wenbin,et al.Bacterial population composition and succession in column bioleaching process of lowgrade copper sulphide ore[J].The Chinese Journal of Process Engineering,2009(1):88-94.
[12]Yaghobi Moghaddam M,Shafaei S Z,Noaparast M,et al.Empirical model for bio-extraction of copper from low grade ore using response surface methodology[J].Transactions of Nonferrous Metals Society of China,2015,25:4126-4143.
[13]张婧,邹平,孙石,等.化学-生物联合浸出次生硫化铜精矿的研究[J].矿冶工程,2015(6):123-127.Zhang Jing,Zou Ping,Sun Peishi,et al.A combined chemical and biological leaching process for secondary copper sulfide concentrate[J].Mining and Metallurgical Engineering.2015(6):123-127.
[14]Abdollahi H,Shafaei S Z,Noaparast M,et al.Bio-dissolution of Cu,Mo and Re from molybdenite concentrate using mix mesophilic microorganism in shake flask[J].Transactions of Nonferrous Metals Society of China,2013,23:219-230.
[15]Zamani M A A,Hiroyoshi N,Tsunekaw A M,et al.Bioleaching of sarheshmeh molybdenum concentrate for extraction of rhenium[J].Hydrometallurgy,2005,80:23-31.