不同贮藏温度对萱草花粉活力的影响
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摘要
为解决萱草杂交育种过程中花期不遇、异地杂交等问题,以‘金娃娃’为试材,采用TTC染色法对‘金娃娃’花粉分别在高温干燥、高温湿润两种不同环境条件下1 d内花粉活力变化进行测定,并研究了25℃、4℃、-20℃、-75℃四个贮藏温度条件对萱草花粉生活力的影响情况。结果表明:1 d内早晨7时萱草花粉活力达到最高值,花粉采集应选择高温湿润的环境条件时为佳;不同贮藏条件下,花粉活力变化有很大差异。常温(25℃)贮藏时,花粉活力下降最快,贮藏28 d后全部丧失活力;4℃贮藏条件下保存7 d时活力可达到68.84%,利于短期贮藏;若长期贮藏,则应选择-75℃的温度条件,-75℃贮藏条件下保存42 d后‘金娃娃’花粉活力仍可达到39.96%。本研究为萱草花粉的种质资源保存和杂交育种工作提供了参考依据。
In order to overcome the problem of asynchronous flowering period and cross-breeding in different places in the process of hybrid breeding of Hemerocallis fulva, using 'Jin Wawa' pollens as materials, the pollen viability of 'Jin Wawa' in one day under two different environmental conditions of high temperature drying and high temperature wetting was tested by means of TTC staining, and effects of storage temperature conditions of25℃, 4℃, 20℃, and-75℃ on Hemerocallis pollen viabilities were researched. The results showed that the pollen viability of Hemerocallis fulva was highest at 7:00 A.M. in a day, and the pollen collection should be preferred when the temperature was high and humidity was wet. The viability of pollens was different under different storage conditions. At room temperature(25℃), pollen vitality decreased fastest, and and all pollen vitality was lost after28 days storage. When stored at 4℃ for 7 d, the pollen vitality could reach 68.84%, which was favorable for short-term storage. If stored for a long time, the pollen vitality of 'Jinwa' could still reach 39.96% after storage at-75℃ for 42 days. This study would provide a reference for gemplasm resources conservation and cross breeding of Hemerocallis fulva.
In order to overcome the problem of asynchronous flowering period and cross-breeding in different places in the process of hybrid breeding of Hemerocallis fulva, using 'Jin Wawa' pollens as materials, the pollen viability of 'Jin Wawa' in one day under two different environmental conditions of high temperature drying and high temperature wetting was tested by means of TTC staining, and effects of storage temperature conditions of25℃, 4℃, 20℃, and-75℃ on Hemerocallis pollen viabilities were researched. The results showed that the pollen viability of Hemerocallis fulva was highest at 7:00 A.M. in a day, and the pollen collection should be preferred when the temperature was high and humidity was wet. The viability of pollens was different under different storage conditions. At room temperature(25℃), pollen vitality decreased fastest, and and all pollen vitality was lost after28 days storage. When stored at 4℃ for 7 d, the pollen vitality could reach 68.84%, which was favorable for short-term storage. If stored for a long time, the pollen vitality of 'Jinwa' could still reach 39.96% after storage at-75℃ for 42 days. This study would provide a reference for gemplasm resources conservation and cross breeding of Hemerocallis fulva.
引文
He Z.M.,and Zhou Z.Y.,2004,The impacts of weather conditions in hybrid rice seed production,Zajiao Shuidao(Hybrid Rice),19(2):43-44(何增明,周宗岳,2004,论杂交水稻制种中的天气影响,杂交水稻,19(2):43-44)
Hu J.Y.,Li Y.,Sun Y.H.,Jiang J.Z.,Cheng J.X.,and Gao S.Y.,2008,Optimization of the pollen vitality identification and storage methods of Ginkgo biloba,Zhongguo Nongxue Tongbao(Chinese Agricultural Science Bulletin),24(5):148-153(胡君艳,李云,孙宇涵,姜金仲,程金新,高尚永,2008,银杏花粉生活力测定及贮藏方法的优化,中国农学通报,24(5):148-153)
Hu L.,Hu B.M.,Wang P.Z.,Shi L.F.,and Chen L.,2009,Impact of low temperature and ultra-low temperature on the cotton pollen viability,Xinjiang Nongye Kexue(Xinjiang Agricultural Sciences),46(5):941-945(胡磊,胡保民,王沛政,史丽芳,陈玲,2009,低温超低温对棉花花粉活力的影响,新疆农业科学,46(5):941-945)
Hu S.Y.,1993,Experimental methods in plant embryology(Ⅰ)determination of pollen viability,Zhiwuxue Tongbao(Chinese Bulletin of Botany),70(2):60-62(胡适宜,1993,植物胚胎学实验方法花粉生活力的测定,植物学通报,70(2):60-62)
Jiang M.T.,2015,Hybridization breeding technics of several species of Melastoma Linn.,Fujian Agriculture and Forestry University,Supervisor:Peng D.H.,pp.24-25(江鸣涛,2015,几种野牡丹属植物杂交育种技术研究,硕士学位论文,福建农林大学,导师:彭东辉,pp.24-25)
Liu X.,Liu S.Y.,Sun Y.Y.,Cao Y.,and Liu H.Z.,2014,Research progress of Hemerocallis,Heilongjiang Nongye Kexue(Heilongjiang Agricultural Sciences),(3):138-141(刘昕,刘树英,孙叶迎,曹岩,刘洪章,2014,萱草属植物研究进展,黑龙江农业科学,(3):138-141)
Shi J.,Xin L.,Shi G.A.,Shi G.A.,Kong X.S.,Gao S.C.,and Huo Z.P.,2009,Pollen storage characteristic of paeonia suffruticosa and under three store temperature,Henan Keji Daxue Xuebao(Journal of Henan University of Science and Technology(Natural Science)),30(3):76-78(施江,辛莉,史国安,孔祥生,高双成,霍志鹏,2009,三种贮藏温度条件下牡丹花粉的储藏特性,河南科技大学学报(自然科学版),30(3):76-78)
Wang L.,Zhu P.F.,and Mao H.Y.,2009,Viability of the pollen of Hemerocallis hybridus under different media and store conditions,Xilin Xueyuan Xuebao(Journal of North West Forestry University),24(3):95-97,108(王玲,祝朋芳,毛洪玉,2009,不同培养基及不同贮藏条件对金娃娃萱草花粉生命力的影响,西林学院学报,24(3):95-97,108)
Zhang Z.X.,Yao J.P.,Li Q.Y.,Zhang L.Y.,Li R.H.,Chen W.F.,Su Y.A.,Wang Y.R.,and Zhang X.,2010,Effects of environmental conditions on in vitro pollen vigor of japonica rice restorer lines,Zajiao Shuidao(Hybrid Rice),25(S):495-498(张忠旭,姚继攀,李全英,张丽颖,李如海,陈温福,苏玉安,王彦荣,张雪,2010,不同环境条件对杂交粳稻恢复系离体花粉活力的影响,杂交水稻,25(S):495-498)
Zhao K.Y.,2013,A study of the viability and storage conditions of Camellia pollen,Sichuan Linye Keji(Journal of Sichuan Forestry Science and Technology),34(5):13-18(赵克跃,2013,山茶花粉生活力及贮藏条件初探,四川林业科技,34(5):13-18)
Zhao W.F.,Xing S.Y.,Jiang Y.X.,and Du C.T.,2004,Effects of storage time on germination percentage and protective enzymes activity of Ginkgo biloba L.pollen,Wuhan Zhiwuxue Yanjiu(Journal of Wuhan Botanical Research),22(3):259-263(赵文飞,邢世岩,姜永旭,杜成涛,2004,贮藏时间对银杏花粉保护酶活性和萌发率的影响,武汉植物学研究,22(3):259-263)
Zhao Y.F.,Lin J.X.,Zhu B.Y.,Kang X.J.,and Yin X.Y.,2017,Pollen viability of 6 Hemerocallis hybrida varieties,Xibu Linye Kexue(Journal of West China Forestry Science),46(2):15-19,31(赵玉芬,李金霞,储博彦,康兴娇,尹新彦,2017,6个品种大花萱草花粉活力的测定,西部林业科学,46(2):15-19,31)
Zhu Y.H.,2010,The genetic relationships among species within Hemerocallis genus and new germplasm selection based on interspecies crossing,Dissertation for Ph.D.,Nanjing Forestry University,Supervisor:Shi J.S.,pp.45-47(朱云华,2010,萱草属(Hemerocallis spp.)种质资源亲缘关系及种内杂交新种质选择,博士学位论文,南京林业大学,导师:施季森,pp.45-47)
Hu J.Y.,Li Y.,Sun Y.H.,Jiang J.Z.,Cheng J.X.,and Gao S.Y.,2008,Optimization of the pollen vitality identification and storage methods of Ginkgo biloba,Zhongguo Nongxue Tongbao(Chinese Agricultural Science Bulletin),24(5):148-153(胡君艳,李云,孙宇涵,姜金仲,程金新,高尚永,2008,银杏花粉生活力测定及贮藏方法的优化,中国农学通报,24(5):148-153)
Hu L.,Hu B.M.,Wang P.Z.,Shi L.F.,and Chen L.,2009,Impact of low temperature and ultra-low temperature on the cotton pollen viability,Xinjiang Nongye Kexue(Xinjiang Agricultural Sciences),46(5):941-945(胡磊,胡保民,王沛政,史丽芳,陈玲,2009,低温超低温对棉花花粉活力的影响,新疆农业科学,46(5):941-945)
Hu S.Y.,1993,Experimental methods in plant embryology(Ⅰ)determination of pollen viability,Zhiwuxue Tongbao(Chinese Bulletin of Botany),70(2):60-62(胡适宜,1993,植物胚胎学实验方法花粉生活力的测定,植物学通报,70(2):60-62)
Jiang M.T.,2015,Hybridization breeding technics of several species of Melastoma Linn.,Fujian Agriculture and Forestry University,Supervisor:Peng D.H.,pp.24-25(江鸣涛,2015,几种野牡丹属植物杂交育种技术研究,硕士学位论文,福建农林大学,导师:彭东辉,pp.24-25)
Liu X.,Liu S.Y.,Sun Y.Y.,Cao Y.,and Liu H.Z.,2014,Research progress of Hemerocallis,Heilongjiang Nongye Kexue(Heilongjiang Agricultural Sciences),(3):138-141(刘昕,刘树英,孙叶迎,曹岩,刘洪章,2014,萱草属植物研究进展,黑龙江农业科学,(3):138-141)
Shi J.,Xin L.,Shi G.A.,Shi G.A.,Kong X.S.,Gao S.C.,and Huo Z.P.,2009,Pollen storage characteristic of paeonia suffruticosa and under three store temperature,Henan Keji Daxue Xuebao(Journal of Henan University of Science and Technology(Natural Science)),30(3):76-78(施江,辛莉,史国安,孔祥生,高双成,霍志鹏,2009,三种贮藏温度条件下牡丹花粉的储藏特性,河南科技大学学报(自然科学版),30(3):76-78)
Wang L.,Zhu P.F.,and Mao H.Y.,2009,Viability of the pollen of Hemerocallis hybridus under different media and store conditions,Xilin Xueyuan Xuebao(Journal of North West Forestry University),24(3):95-97,108(王玲,祝朋芳,毛洪玉,2009,不同培养基及不同贮藏条件对金娃娃萱草花粉生命力的影响,西林学院学报,24(3):95-97,108)
Zhang Z.X.,Yao J.P.,Li Q.Y.,Zhang L.Y.,Li R.H.,Chen W.F.,Su Y.A.,Wang Y.R.,and Zhang X.,2010,Effects of environmental conditions on in vitro pollen vigor of japonica rice restorer lines,Zajiao Shuidao(Hybrid Rice),25(S):495-498(张忠旭,姚继攀,李全英,张丽颖,李如海,陈温福,苏玉安,王彦荣,张雪,2010,不同环境条件对杂交粳稻恢复系离体花粉活力的影响,杂交水稻,25(S):495-498)
Zhao K.Y.,2013,A study of the viability and storage conditions of Camellia pollen,Sichuan Linye Keji(Journal of Sichuan Forestry Science and Technology),34(5):13-18(赵克跃,2013,山茶花粉生活力及贮藏条件初探,四川林业科技,34(5):13-18)
Zhao W.F.,Xing S.Y.,Jiang Y.X.,and Du C.T.,2004,Effects of storage time on germination percentage and protective enzymes activity of Ginkgo biloba L.pollen,Wuhan Zhiwuxue Yanjiu(Journal of Wuhan Botanical Research),22(3):259-263(赵文飞,邢世岩,姜永旭,杜成涛,2004,贮藏时间对银杏花粉保护酶活性和萌发率的影响,武汉植物学研究,22(3):259-263)
Zhao Y.F.,Lin J.X.,Zhu B.Y.,Kang X.J.,and Yin X.Y.,2017,Pollen viability of 6 Hemerocallis hybrida varieties,Xibu Linye Kexue(Journal of West China Forestry Science),46(2):15-19,31(赵玉芬,李金霞,储博彦,康兴娇,尹新彦,2017,6个品种大花萱草花粉活力的测定,西部林业科学,46(2):15-19,31)
Zhu Y.H.,2010,The genetic relationships among species within Hemerocallis genus and new germplasm selection based on interspecies crossing,Dissertation for Ph.D.,Nanjing Forestry University,Supervisor:Shi J.S.,pp.45-47(朱云华,2010,萱草属(Hemerocallis spp.)种质资源亲缘关系及种内杂交新种质选择,博士学位论文,南京林业大学,导师:施季森,pp.45-47)