大片吸虫成虫体被结构的扫描电镜和透射电镜观察
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摘要
目的了解大片吸虫的超微结构特征并分析其功能。方法采用扫描电镜技术对大片吸虫的成虫体表超微形态结构进行连续观察,应用透射电镜对大片吸虫的体被组织结构进行观察。结果大片吸虫虫体体表覆盖有体棘,体棘的前端分叉成锯齿状;虫体的感觉乳突有的呈多个聚集分布,而有的为单个分布。透射电镜下,虫体可分为体被、肌层和细胞体层。体被为一合体层,厚度约25μm,包括外质膜、基质和基底膜;基质富含分泌颗粒、线粒体、空泡。体被的最外层观察到糖萼和质膜凹陷,可能与虫体的免疫逃避和营养吸收有关。肌层分布有环形和纵行两种肌肉,肌肉层的肌肉间隙和原生质管可能是输送营养物质的通道,质管内的分泌颗粒和小空泡可能为主要的营养物质。细胞体层内细胞核清晰,细胞质内有线粒体、胞质空泡和分泌颗粒。结论大片吸虫成虫体被可能是成虫逃避免疫攻击和营养吸收的重要部位。
引文
[1]王冬英,张为宇,黄维义.广西牛片形吸虫流行情况调查[J].广西畜牧兽医,2006,22(5):200-202.
[2]Le TH,De NV,Agatsuma T,et al.Molecular confirmation that Fasciola gigantica can undertake aberrant migrations in human Hosts[J].J Clin Microbiol.2007,45(2):648-650.
[3]黄正美,邢兰燕,李六九,等.肝片吸虫病2例报告[J].寄生虫病与感染性疾病,2009,7(3):172.
[4]吕善根,钟炎皋,秦保贵.肝片吸虫病2例报告[J].临床儿科杂志,1987,5(3):149-150.
[5]陈木新,艾琳,许学年,等.云南省大理州大片形吸虫群体感染26例分析[J].中华地方病学杂志,2012,31(6):595-598.
[6]Winkelhagen AJ,Mank T,de Vries PJ,et al.Apparent triclabendazole-resistant human Fasciola hepatica infection,the netherlands[J].Emerg Infect Dis,2012,18(6):1028-1029.
[7]Brockwell YM,Elliott TP,Anderson GR,et al.Confirmation of Fasciola hepatica resistant to triclabendazole in naturally infected Australian beef and dairy cattle[J].Int J Parasitol Drugs Drug Resist.,2014,4(1):48-54.
[8]Kelley JM,Elliott TP,Beddoe T,et al.Current threat of triclabendazole resistance in Fasciola hepatica[J].Trends Parasitol,2016,32(6):458-469.
[9]Bouvard V,Baan R,Straif K,et al.A review of human carcinogens-Part B:biological agents[J].Lancet oncol.2009,10(4):321-322.
[10]Dalton JP,Skelly P,Halton DW.Role of the tegument and gut in nutrient uptake by parasitic platyhelminths[J].Can J Zoo,2004,82(2):211-232.
[11]Mc Laren DJ,Terry RJ.The protective role of acquired host antigens during schistosome maturation[J].Parasite Immunol,1982,4(2):129-148.
[12]Wilson RA,Wright JM,de Castro-Borges W,et al.Exploring the Fasciola hepatica tegument proteome.[J].Int J Parasitol,2011,41(13-14):1347-1359.
[13]Mulvenna J,Moertel L,Jones MK,et al.Exposed proteins of the Schistosoma japonicum tegument.[J].Int J Parasitol,2010,40(5):543-554.
[14]Braschi S,Borges WC,Wilson RA.Proteomic analysis of the schistosome tegument and its surface membranes[J].Memóriasdo Instituto Oswaldo Cruz,2006,101(4):205-212.
[15]程由注,许国防,杨文川,等.东方次睾吸虫成虫体被扫描电镜研究[J].中国人兽共患病学报,2005,21(10):875-879.
[16]徐国余,李解权,濮祖茂,等.南京血吸虫扫描电镜观察[J].中国血吸虫病防治杂志,2005,17(6):449-450.
[17]Pearce EJ,Basch PF,Sher A.Evidence that the reduced surface antigenicity of developing Schistosoma mansoni schistosomula is due to antigen shedding rather than host molecule acquisition[J].Parasite Immunol.1986,8(1):79-94.
[18]Wilson RA,Barnes PE.The formation and turnover of the membranocalyx on the tegument of Schistosoma mansoni[J].Parasitology,1977,74(1):61-71.
[19]Zhang WY,Moreau E,Yang B Z,et al.Humoral and cellular immune responses to Fasciola gigantica experimental infection in buffaloes[J].Res Vet Sci,2006,80(3):299-307.
[20]Zhang WY,Moreau E,Hope JC,et al.Fasciola hepatica and Fasciola gigantica:comparison of cellular response to experimental infection in sheep[J].Exp Parasitol,2005,111(3):154-159.
[2]Le TH,De NV,Agatsuma T,et al.Molecular confirmation that Fasciola gigantica can undertake aberrant migrations in human Hosts[J].J Clin Microbiol.2007,45(2):648-650.
[3]黄正美,邢兰燕,李六九,等.肝片吸虫病2例报告[J].寄生虫病与感染性疾病,2009,7(3):172.
[4]吕善根,钟炎皋,秦保贵.肝片吸虫病2例报告[J].临床儿科杂志,1987,5(3):149-150.
[5]陈木新,艾琳,许学年,等.云南省大理州大片形吸虫群体感染26例分析[J].中华地方病学杂志,2012,31(6):595-598.
[6]Winkelhagen AJ,Mank T,de Vries PJ,et al.Apparent triclabendazole-resistant human Fasciola hepatica infection,the netherlands[J].Emerg Infect Dis,2012,18(6):1028-1029.
[7]Brockwell YM,Elliott TP,Anderson GR,et al.Confirmation of Fasciola hepatica resistant to triclabendazole in naturally infected Australian beef and dairy cattle[J].Int J Parasitol Drugs Drug Resist.,2014,4(1):48-54.
[8]Kelley JM,Elliott TP,Beddoe T,et al.Current threat of triclabendazole resistance in Fasciola hepatica[J].Trends Parasitol,2016,32(6):458-469.
[9]Bouvard V,Baan R,Straif K,et al.A review of human carcinogens-Part B:biological agents[J].Lancet oncol.2009,10(4):321-322.
[10]Dalton JP,Skelly P,Halton DW.Role of the tegument and gut in nutrient uptake by parasitic platyhelminths[J].Can J Zoo,2004,82(2):211-232.
[11]Mc Laren DJ,Terry RJ.The protective role of acquired host antigens during schistosome maturation[J].Parasite Immunol,1982,4(2):129-148.
[12]Wilson RA,Wright JM,de Castro-Borges W,et al.Exploring the Fasciola hepatica tegument proteome.[J].Int J Parasitol,2011,41(13-14):1347-1359.
[13]Mulvenna J,Moertel L,Jones MK,et al.Exposed proteins of the Schistosoma japonicum tegument.[J].Int J Parasitol,2010,40(5):543-554.
[14]Braschi S,Borges WC,Wilson RA.Proteomic analysis of the schistosome tegument and its surface membranes[J].Memóriasdo Instituto Oswaldo Cruz,2006,101(4):205-212.
[15]程由注,许国防,杨文川,等.东方次睾吸虫成虫体被扫描电镜研究[J].中国人兽共患病学报,2005,21(10):875-879.
[16]徐国余,李解权,濮祖茂,等.南京血吸虫扫描电镜观察[J].中国血吸虫病防治杂志,2005,17(6):449-450.
[17]Pearce EJ,Basch PF,Sher A.Evidence that the reduced surface antigenicity of developing Schistosoma mansoni schistosomula is due to antigen shedding rather than host molecule acquisition[J].Parasite Immunol.1986,8(1):79-94.
[18]Wilson RA,Barnes PE.The formation and turnover of the membranocalyx on the tegument of Schistosoma mansoni[J].Parasitology,1977,74(1):61-71.
[19]Zhang WY,Moreau E,Yang B Z,et al.Humoral and cellular immune responses to Fasciola gigantica experimental infection in buffaloes[J].Res Vet Sci,2006,80(3):299-307.
[20]Zhang WY,Moreau E,Hope JC,et al.Fasciola hepatica and Fasciola gigantica:comparison of cellular response to experimental infection in sheep[J].Exp Parasitol,2005,111(3):154-159.