摘要
目的了解大片吸虫的超微结构特征并分析其功能。方法采用扫描电镜技术对大片吸虫的成虫体表超微形态结构进行连续观察,应用透射电镜对大片吸虫的体被组织结构进行观察。结果大片吸虫虫体体表覆盖有体棘,体棘的前端分叉成锯齿状;虫体的感觉乳突有的呈多个聚集分布,而有的为单个分布。透射电镜下,虫体可分为体被、肌层和细胞体层。体被为一合体层,厚度约25μm,包括外质膜、基质和基底膜;基质富含分泌颗粒、线粒体、空泡。体被的最外层观察到糖萼和质膜凹陷,可能与虫体的免疫逃避和营养吸收有关。肌层分布有环形和纵行两种肌肉,肌肉层的肌肉间隙和原生质管可能是输送营养物质的通道,质管内的分泌颗粒和小空泡可能为主要的营养物质。细胞体层内细胞核清晰,细胞质内有线粒体、胞质空泡和分泌颗粒。结论大片吸虫成虫体被可能是成虫逃避免疫攻击和营养吸收的重要部位。
引文
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