猕猴桃皮中6种多酚类化合物的HPLC检测法建立
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摘要
建立使用高效液相色谱法同时测定猕猴桃皮中6种多酚类化合物含量的方法。检测方法为:色谱柱为InertSustain C18(4.6 mm×250 mm,5μm),以甲醇-10 mmoL/L KH_2PO_4水溶液(pH2.0)为流动相进行梯度洗脱,柱温40℃,流速1 m L/min,进样量10μL,检测波长为280 nm。结果表明:此方法可使6种目标组分在50 min内得到良好的分离,且方法重复性好(RSD≤1.40%)、稳定性较强(RSD≤2.86%)、精密度较高(RSD≤1.85%)、回收率在84.90～108.78%之间。该方法操作简单、快速,能为猕猴桃皮多酚的相关研究提供有效数据。
The simultaneous determination method of six kinds of polyphenols content in kiwifruit peel was made by using the high performance liquid chromatography( HPLC).In terms of the specific approach,the InertSustain C18( 4.6 mm × 250 mm,5 μm) was applied to the chromatographic column. The gradient elution was carried out by using methanol-10 mmoL/L KH_2PO_4 aqueous solution( pH2.0) as mobile phase.The column temperature was 40 ℃,the flow rate was 1 m L/min,the sample volume was 10 μL,and the detection wavelength was detected at 280 nm. Results showed that this method could be used for separating the six target components well in 50 min.The approach had great reproducibility( RSD≤ 1.40%),strong stability( RSD≤ 2.86%) and high precision( RSD≤1.85%).Furthermore,the recovery rate was between 84.90% and 108.78%.Therefore,this was a simple and quick operation method which could effectively provide relevant data for the research of polyphones in kiwifruit peel.
The simultaneous determination method of six kinds of polyphenols content in kiwifruit peel was made by using the high performance liquid chromatography( HPLC).In terms of the specific approach,the InertSustain C18( 4.6 mm × 250 mm,5 μm) was applied to the chromatographic column. The gradient elution was carried out by using methanol-10 mmoL/L KH_2PO_4 aqueous solution( pH2.0) as mobile phase.The column temperature was 40 ℃,the flow rate was 1 m L/min,the sample volume was 10 μL,and the detection wavelength was detected at 280 nm. Results showed that this method could be used for separating the six target components well in 50 min.The approach had great reproducibility( RSD≤ 1.40%),strong stability( RSD≤ 2.86%) and high precision( RSD≤1.85%).Furthermore,the recovery rate was between 84.90% and 108.78%.Therefore,this was a simple and quick operation method which could effectively provide relevant data for the research of polyphones in kiwifruit peel.
引文
[1]吴怀恩,甄汉深,钟振国.猕猴桃属植物的研究进展[J].中药材,2004,27(1):59-63.
[2]任晓婷,张生万,李美萍,等.不同品种猕猴桃总酚含量与清除自由基能力相关性研究[J].山西农业大学学报,2016,36(5):341-344.
[3]唐瑶,陈洋,曹婉鑫.多酚类化合物的分类、来源及功能研究进展[J].中国食物与营养,2016,22(3):32-34.
[4]柯薇.香蕉皮中多酚类物质的抑菌活性研究[J].工程技术,2016,15(39):93-95.
[5]周凯.龙眼核多酚的分离纯化及其对小鼠烫伤愈合的影响[D].广州:华南农业大学,2016.
[6]游庭活.桑椹多酚类化合物抗氧化及延缓衰老作用研究[D].广州:广东药学院,2015.
[7]杨谷良,黄春保,潘敏雄,等.甘蔗多酚对胆固醇诱导高血脂大鼠的影响[J].江西农业大学学报,2017,39(5):996-1001.
[8]左丽丽.狗枣猕猴桃多酚的抗氧化与抗肿瘤效应研究[D].哈尔滨:哈尔滨工业大学,2013.
[9]罗剑鸣.葡萄皮渣酚类化合物体外抑制乳腺癌的功效及机理[D].北京:中国农业大学,2017.
[10]左丽丽,王振宇,卢静,等.猕猴桃多酚的生理功能研究进展[J].食品研究与开发,2013,34(11):117-127.
[11]杨建荣.苹果多酚的高效液相色谱检测方法的建立[D].济南:山东大学,2006.
[12]柳伟,肖雪,葛珊珊,等.12种果皮多酚含量及其抗氧化活性研究[J].食品研究与开发,2016,37(14):25-29.
[13]赵谋明,董红竹,林恋竹.八种水果多酚的定量分析与抗氧化活性研究[J].现代食品科技,2017,33(10):225-236.
[14]李志君,万红焱,顾丽莉,等.草果多酚物质提取及LC-MS/MS分析[J].食品工业科技,2017,32(8):294-334.
[15]姜莉,王玉堂,周元,等.红米酒中多酚类化合物的高效液相色谱法测定[J].食品研究与开发,2015,36(17):129-132.
[16]卢文青,周晓敏,张怀玉,等.高效液相色谱法同时测定水果中9种多酚物质[J].中国卫生检验杂志,2017,27(3):317-320.
[17]张春梅,黄玉林,程芳芳,等.槟榔花提取物中没食子酸等9种多酚类化合物的测定[J].热带作物学报,2011,32(5):965-969.
[18]邓佩欣,周文财,高雅媛,等.HPLC法同时测定石榴皮中4种多酚类成分的含量[J].广州化工,2017,45(2):107-109.
[19]邓俊琳,李旭.油橄榄鲜果中多酚化合物含量测定的HPLC法研究[J].西北农林科技大学学报,2017,45(1):185-189.
[20]高振鹏.猕猴桃中多酚的提取条件优化及其动力学模型构建[D].南京:南京农业大学,2014.
[21]杜丽娟,薛洁,王异静.反相高效液相色谱法测定猕猴桃果酒的多酚物质[J].酿酒,2008,35(1):72-74.
[22]李学鹏,励建荣,杨建荣.苹果中多酚类物质HPLC检测方法的建立[J].中国食品学报,2008,8(6):116-121.
[2]任晓婷,张生万,李美萍,等.不同品种猕猴桃总酚含量与清除自由基能力相关性研究[J].山西农业大学学报,2016,36(5):341-344.
[3]唐瑶,陈洋,曹婉鑫.多酚类化合物的分类、来源及功能研究进展[J].中国食物与营养,2016,22(3):32-34.
[4]柯薇.香蕉皮中多酚类物质的抑菌活性研究[J].工程技术,2016,15(39):93-95.
[5]周凯.龙眼核多酚的分离纯化及其对小鼠烫伤愈合的影响[D].广州:华南农业大学,2016.
[6]游庭活.桑椹多酚类化合物抗氧化及延缓衰老作用研究[D].广州:广东药学院,2015.
[7]杨谷良,黄春保,潘敏雄,等.甘蔗多酚对胆固醇诱导高血脂大鼠的影响[J].江西农业大学学报,2017,39(5):996-1001.
[8]左丽丽.狗枣猕猴桃多酚的抗氧化与抗肿瘤效应研究[D].哈尔滨:哈尔滨工业大学,2013.
[9]罗剑鸣.葡萄皮渣酚类化合物体外抑制乳腺癌的功效及机理[D].北京:中国农业大学,2017.
[10]左丽丽,王振宇,卢静,等.猕猴桃多酚的生理功能研究进展[J].食品研究与开发,2013,34(11):117-127.
[11]杨建荣.苹果多酚的高效液相色谱检测方法的建立[D].济南:山东大学,2006.
[12]柳伟,肖雪,葛珊珊,等.12种果皮多酚含量及其抗氧化活性研究[J].食品研究与开发,2016,37(14):25-29.
[13]赵谋明,董红竹,林恋竹.八种水果多酚的定量分析与抗氧化活性研究[J].现代食品科技,2017,33(10):225-236.
[14]李志君,万红焱,顾丽莉,等.草果多酚物质提取及LC-MS/MS分析[J].食品工业科技,2017,32(8):294-334.
[15]姜莉,王玉堂,周元,等.红米酒中多酚类化合物的高效液相色谱法测定[J].食品研究与开发,2015,36(17):129-132.
[16]卢文青,周晓敏,张怀玉,等.高效液相色谱法同时测定水果中9种多酚物质[J].中国卫生检验杂志,2017,27(3):317-320.
[17]张春梅,黄玉林,程芳芳,等.槟榔花提取物中没食子酸等9种多酚类化合物的测定[J].热带作物学报,2011,32(5):965-969.
[18]邓佩欣,周文财,高雅媛,等.HPLC法同时测定石榴皮中4种多酚类成分的含量[J].广州化工,2017,45(2):107-109.
[19]邓俊琳,李旭.油橄榄鲜果中多酚化合物含量测定的HPLC法研究[J].西北农林科技大学学报,2017,45(1):185-189.
[20]高振鹏.猕猴桃中多酚的提取条件优化及其动力学模型构建[D].南京:南京农业大学,2014.
[21]杜丽娟,薛洁,王异静.反相高效液相色谱法测定猕猴桃果酒的多酚物质[J].酿酒,2008,35(1):72-74.
[22]李学鹏,励建荣,杨建荣.苹果中多酚类物质HPLC检测方法的建立[J].中国食品学报,2008,8(6):116-121.