大鼠肾移植急性排斥反应过程中IL-17表达及其意义
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摘要
目的:探讨IL-17在大鼠肾移植急性排斥反应过程中的表达特点及其意义。方法:将大鼠随机分为正常对照组、同系基因对照组、同种异体移植组、免疫抑制剂干预组,并建立肾移植急性排斥反应模型,用RT-PCR及免疫组织化学方法检测急性排斥时IL-17在肾组织中的表达。结果:同种异体移植组在移植后第3天、5天和7天,移植肾IL-17均明显升高,与其他组相比,均有统计学意义(P<0. 05),其中以第5天为最高,差异具有统计学有意义(P<0. 05)。结论:IL-17参与了大鼠肾移植急性排斥反应的发生,并为早期事件,IL-17检测可能为人类肾移植早期急性排斥反应的诊断和治疗提供理论依据。
Objective:To investigate the expression characteristics and significance of IL-17 in acute rejection of renal transplantation in rats. Methods: Rats were randomly divided into normal control group,homogeneic control group,allogeneic transplantation group and immunosuppressant intervention group,and acute rejection reaction model of renal transplantation was established. The expression of IL-17 in renal tissue during acute rejection was detected by RT-PCR and immunohistochemistry. Results: IL-17 was significantly increased in the allograft group on the 3 rd,5 th and 7 th days after transplantation. Compared with other groups,IL-17 was statistically significant( P<0. 05),of which the 5 th day was the highest and statistically significant( P<0. 05). Conclusion: IL-17 is involved in the occurrence of acute rejection of renal transplantation in rats,and may provide theoretical basis for the diagnosis and treatment of acute rejection in early stage of human kidney transplantation.
Objective:To investigate the expression characteristics and significance of IL-17 in acute rejection of renal transplantation in rats. Methods: Rats were randomly divided into normal control group,homogeneic control group,allogeneic transplantation group and immunosuppressant intervention group,and acute rejection reaction model of renal transplantation was established. The expression of IL-17 in renal tissue during acute rejection was detected by RT-PCR and immunohistochemistry. Results: IL-17 was significantly increased in the allograft group on the 3 rd,5 th and 7 th days after transplantation. Compared with other groups,IL-17 was statistically significant( P<0. 05),of which the 5 th day was the highest and statistically significant( P<0. 05). Conclusion: IL-17 is involved in the occurrence of acute rejection of renal transplantation in rats,and may provide theoretical basis for the diagnosis and treatment of acute rejection in early stage of human kidney transplantation.
引文
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[9]Hsieh HG,Loong CC,Lui WY,et al.IL-17 expression as a possible predictive parameter for subclinical renal allograft rejection[J].Transplant International,2001,14(5):287-298.
[10]Kim N,Yoon YI,Yoo Hj,et al.Combined detection of serum IL-10,IL-17,and CXCL10 predicts acute rejection following adult liver transplantation[J].Mol Cells,2016,39(8):639-644.
[11]Woltman AM,de Hai j S,Boonstra JG,et al.Interleukin-17 and CD40-ligand synergistically enhance cytokine and chemokine production by renal epithelial cells[J].Am Soc Nephrol,2000,11(11):2044-2055.
[12]Wang ZH,Li YZ,Wang ZK.Significance of Th-17 and IL-17 to acute rejection following liver transplantation[J].Chin JMicroecol,2014,26(5):567-570.
[13]Loong CC,Lin CY,Lui WY.Expression of interleukin-17 as a predictive parameter in acute renal allograft rejection[J].Transplant Proc,2000,32(7):1773.
[2]李艳双,宋晓天,张征峥,等.转染IL-17基因的小鼠结肠癌细胞体内抗肿瘤机制研究[J].中国免疫学杂志,2015,31(5):643-649.Li YS,Song XT,Zhang ZZ,et al.Antitumor mechanism of mouse colon cancer cells transfected with IL-17 gene in vivo[J].Chin JImmunol,2015,31(5):643-649.
[3]Krisl JC,Alloway RR,Shield AR,et al.Acute rejection clinically defined phenotypes correlate with long-term renal allograft survival[J].Transplantation,2015,99(10):2167-2173.
[4]Jalalzadeh M,Mousavinasab N,Peyrovi S,et al.The impact of acute rejection in kidney transplantation on long-term allograft and patient outcome[J].Nephrourol Mon,2015,7(1):e24439.
[5]Jafari A,Khatami MR,Dashti-Khavidaki S,et al.Plasma neutrophil gelatinase-associated lipocalin as a marker for prediction of 3-moth graft survival after kidney transplantation[J].Int J Organ Transplant Med,2017,8(1):17-27.
[6]Chung BH,Oh HJ,Piao SG,et al.Clinical significance of the ratio between FOXP3 positive regulatory T cell and interleukin-17 secreting cell in renal allograft biopsies with acute T-cell-mediated rejection[J].Immunology,2012,136(3):344-351.
[7]Chung BH,Oh HJ,Piao SG,et al.Higher infiltration by Th17 cells compared with regulatory T cells is associated with severe acute T-cell-mediated graft rejection[J].Exp Mol Med,2011,43(11):630-637.
[8]Loong CC,Hsieh HG,Lui WY,et al.Evidence for the early involvement of interleukin 17 in human and experimental renal allograft rejection[J].J Pathol,2002,197(3):322-332.
[9]Hsieh HG,Loong CC,Lui WY,et al.IL-17 expression as a possible predictive parameter for subclinical renal allograft rejection[J].Transplant International,2001,14(5):287-298.
[10]Kim N,Yoon YI,Yoo Hj,et al.Combined detection of serum IL-10,IL-17,and CXCL10 predicts acute rejection following adult liver transplantation[J].Mol Cells,2016,39(8):639-644.
[11]Woltman AM,de Hai j S,Boonstra JG,et al.Interleukin-17 and CD40-ligand synergistically enhance cytokine and chemokine production by renal epithelial cells[J].Am Soc Nephrol,2000,11(11):2044-2055.
[12]Wang ZH,Li YZ,Wang ZK.Significance of Th-17 and IL-17 to acute rejection following liver transplantation[J].Chin JMicroecol,2014,26(5):567-570.
[13]Loong CC,Lin CY,Lui WY.Expression of interleukin-17 as a predictive parameter in acute renal allograft rejection[J].Transplant Proc,2000,32(7):1773.