替米沙坦对病毒性心肌炎小鼠炎性损伤的影响
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摘要
目的探究替米沙坦对病毒性心肌炎小鼠炎性损伤的改善作用及机制。方法用腹腔接种柯萨奇病毒B3(CVB3)的方法构建病毒性心肌炎小鼠模型,然后随机分为模型组(n=20)及实验组(n=20),另选20只雄性BALB/c小鼠为对照组。实验组予以灌胃替米沙坦10 mg·kg~(-1),模型组及对照组则灌胃等体积0. 9%Na Cl,共4周。比较3组小鼠心肌组织炎性因子、脂联素(APN)含量及心肌组织APN蛋白表达。结果给药4周后,对照组、模型组和实验组心肌组织匀浆中IL-6分别为(56. 44±12. 41),(137. 06±16. 82),(83. 21±14. 21)pg·mg~(-1); TNF-α分别为(23. 84±8. 54),(105. 41±14. 08),(44. 95±12. 01)pg·mg~(-1); APN分别为(7. 11±0. 08),(5. 94±0. 09),(8. 03±0. 07)μg·mg~(-1),差异均有统计学意义(均P <0. 05)。结论替米沙坦可有效改善病毒性心肌炎小鼠炎性损伤,其作用机制可能与上调心肌组织APN表达相关。
Objective To explore the effect of telmisartan on the inflammatory injury of viral myocarditis mice and the underlying mechanism.Methods Viral myocarditis model was established by Coxsackievirus B3( CVB3) intraperitoneally inoculation. The mice were randomly divided into model group( n = 20) and test group( n = 20). A total of 20 male BALB/c mice were assigned to control group. The test group was given oral administration of telmisartan 10 mg·kg~(-1) once daily,while the model group and the control group were treated with equal amount of 0. 9%NaCl for 4 weeks. The inflammatory factors,adiponectin( APN) content and APN protein expression were compared among 3 groups. Results After 4 weeks of treatment,there were significant differences among the control group,model group and test group in APN [( 7. 11 ± 0. 08)μg·mg~(-1) vs( 5. 94 ± 0. 09) μg·mg~(-1) vs( 8. 03 ± 0. 07) μg·mg~(-1)],IL-6 [( 56. 44 ± 12. 41) pg·mg~(-1) vs( 137. 06 ± 16. 82) pg ·mg~(-1) vs( 83. 21 ± 14. 21) pg·mg~(-1)],TNF-α [( 23. 84 ± 8. 54) pg·mg~(-1) vs( 105. 41 ± 14. 08) pg · mg~(-1) vs( 44. 95 ± 12. 01) pg · mg~(-1)( P < 0. 05). Conclusion Telmisartan can improve the inflammatory injury of viral myocarditis mice effectively,and the action mechanism maybe related to the up-regulation of APN protein expression in myocardial tissue.
Objective To explore the effect of telmisartan on the inflammatory injury of viral myocarditis mice and the underlying mechanism.Methods Viral myocarditis model was established by Coxsackievirus B3( CVB3) intraperitoneally inoculation. The mice were randomly divided into model group( n = 20) and test group( n = 20). A total of 20 male BALB/c mice were assigned to control group. The test group was given oral administration of telmisartan 10 mg·kg~(-1) once daily,while the model group and the control group were treated with equal amount of 0. 9%NaCl for 4 weeks. The inflammatory factors,adiponectin( APN) content and APN protein expression were compared among 3 groups. Results After 4 weeks of treatment,there were significant differences among the control group,model group and test group in APN [( 7. 11 ± 0. 08)μg·mg~(-1) vs( 5. 94 ± 0. 09) μg·mg~(-1) vs( 8. 03 ± 0. 07) μg·mg~(-1)],IL-6 [( 56. 44 ± 12. 41) pg·mg~(-1) vs( 137. 06 ± 16. 82) pg ·mg~(-1) vs( 83. 21 ± 14. 21) pg·mg~(-1)],TNF-α [( 23. 84 ± 8. 54) pg·mg~(-1) vs( 105. 41 ± 14. 08) pg · mg~(-1) vs( 44. 95 ± 12. 01) pg · mg~(-1)( P < 0. 05). Conclusion Telmisartan can improve the inflammatory injury of viral myocarditis mice effectively,and the action mechanism maybe related to the up-regulation of APN protein expression in myocardial tissue.
引文
[1]罗兰,孙文娟,赖舒.缬沙坦对病毒性心肌炎小鼠脂联素及TGF-β1,periostin表达影响[J].中国分子心脏病学杂志,2017,17(3):2124-2127.
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[5]张新民,陈鹏,叶盛,等.蛋白酶体抑制剂减少病毒性心肌炎小鼠炎症因子IL-6和TNF-α的表达[J].中国临床药理学与治疗学,2017,22(4):406-411.
[6]潘红英,徐晓阳,孙涛.游泳运动对动脉粥样硬化小鼠心肌脂联素,PPARα和AMPK表达的影响[J].沈阳体育学院学报,2015,34(2):77-81.
[7]王军伟,杨光,解翠,等.炎琥宁注射液联合抗病毒治疗邪毒侵心型急性病毒性心肌炎的疗效影响[J].世界中医药,2017,12(12):2988-2991.
[8]渠翔,胡德荣,赵雪,等.替米沙坦对脂多糖诱导的抑郁模型大鼠行为,脑内炎症反应及BDNF信号通路的影响[J].中南药学,2017,15(2):162-165.
[9]JENKE A,STEHR J,WILK S,et al.Abstract 14285:Adiponectin promotes development of coxsackievirus B3 myocarditis by suppressing the acute antiviral immune response[J].Archiv Für Kreislaufforschung,2014,109(3):1-16.
[2]杨蓉,何翠竹,王亚玲,等.替米沙坦对病毒性心肌炎小鼠的心肌保护作用研究[J].中华医院感染学杂志,2016,26(6):1319-1321.
[3]娄序笙,胡京红,葛东宇,等.金银花对病毒性心肌炎小鼠的保护作用及其机制[J].中医药学报,2017,45(1):37-41.
[4]运锋,田源,陈会君.苏木乙酸乙酯提取物对急性病毒性心肌炎小鼠模型心肌组织IFN-γ的影响[J].中医药学报,2016,44(6):27-30.
[5]张新民,陈鹏,叶盛,等.蛋白酶体抑制剂减少病毒性心肌炎小鼠炎症因子IL-6和TNF-α的表达[J].中国临床药理学与治疗学,2017,22(4):406-411.
[6]潘红英,徐晓阳,孙涛.游泳运动对动脉粥样硬化小鼠心肌脂联素,PPARα和AMPK表达的影响[J].沈阳体育学院学报,2015,34(2):77-81.
[7]王军伟,杨光,解翠,等.炎琥宁注射液联合抗病毒治疗邪毒侵心型急性病毒性心肌炎的疗效影响[J].世界中医药,2017,12(12):2988-2991.
[8]渠翔,胡德荣,赵雪,等.替米沙坦对脂多糖诱导的抑郁模型大鼠行为,脑内炎症反应及BDNF信号通路的影响[J].中南药学,2017,15(2):162-165.
[9]JENKE A,STEHR J,WILK S,et al.Abstract 14285:Adiponectin promotes development of coxsackievirus B3 myocarditis by suppressing the acute antiviral immune response[J].Archiv Für Kreislaufforschung,2014,109(3):1-16.