生物样品中硒的形态分析方法研究进展
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摘要
对近年来关于生物样品中微量元素硒的形态分析方法的研究成果进行了综述,总结了高硒生物样品(>10 mg Se/kg)和低硒生物样品(<1 mg Se/kg)的硒形态组分提取方法,以及利用液相色谱-原子荧光光谱(LC-AFS)、高效液相色谱与电感耦合等离子质谱(HPLC-ICP-MS)等仪器联用的方法分离、检测硒的形态组分。此外,对利用同步辐射X射线微区分析方法(STXM)原位微区分析微生物样品的硒形态及其分布进行了综述与展望。
Based on recent researches on selenium(Se) speciation analysis on biological samples,the modified extraction methods on Se speciation and composition from high Se contents samples(>10 mg Se/kg) and low Se contents samples(<1 mg Se/kg) were summarized,respectively. The liquid chromatography-atomic fluorescence spectrometry(LC-AFS) and high performance liquid chromatography-inductively coupled plasma-mass spectrometry(HPLC-ICP-MS) employed to separate and determine the Se speciation and composition were discussed. Furthermore,the synchrotron radiation scanning transmission X-ray microscopy(STXM) method was reviewed and prospected on total Se and Se speciation distributions in situ on bacteria samples.
Based on recent researches on selenium(Se) speciation analysis on biological samples,the modified extraction methods on Se speciation and composition from high Se contents samples(>10 mg Se/kg) and low Se contents samples(<1 mg Se/kg) were summarized,respectively. The liquid chromatography-atomic fluorescence spectrometry(LC-AFS) and high performance liquid chromatography-inductively coupled plasma-mass spectrometry(HPLC-ICP-MS) employed to separate and determine the Se speciation and composition were discussed. Furthermore,the synchrotron radiation scanning transmission X-ray microscopy(STXM) method was reviewed and prospected on total Se and Se speciation distributions in situ on bacteria samples.
引文
[1]袁丽君,袁林喜,尹雪斌,等.硒的生理功能、摄入现状与对策研究进展[J].生物技术进展,2016,6(6):396-405.
[2]吕清慧.食品中砷和硒形态的HPLC-ICP-MS分析方法研究[D].天津:天津科技大学,硕士学位论文,2014.
[3]铁梅,方禹之,孙铁彪,等.HPLC-ICP-MS联用技术在富硒金针菇硒的形态分析中的应用[J].高等学校化学学报,2007,28(4):635-639.
[4]Yuan L X,Zhu Y Y,Lin Z Q,et al..A novel selenocystineaccumulating plant in selenium-mine drainage area in Enshi,China[J].PLo S ONE,2013,8(6):e65615.
[5]Banuelos G S,Stushnoff C,Walse S S,et al..Biofortified,selenium enriched,fruit and cladode from three Opuntia cactus pear cultivars grown on agricultural drainage sediment for use in nutraceutical foods[J].Food Chem.,2012,135(1):9-16.
[6]Banuelos G S,Arroyo I,Pickering I J,et al..Selenium biofortification of broccoli and carrots grown in soil amended with Seenriched hyperaccumulator Stanleya pinnata[J].Food Chem.,2015,166:603-608.
[7]杨红霞.镉形态分析与微区分布的质谱联用技术方法研究及其在印度芥菜耐镉机制中的应用[D].北京:中国地质科学院,博士学位论文,2013.
[8]Kulp T R,Pratt L M.Speciation and weathering of selenium in Upper Cretaceous chalk and shale from South Dakota and Wyoming,USA[J].Geochim.Cosmochimi.Acta,2004,68(18):3687-3701.
[9]李璐,秦海波,苏惠,等.硒连续化学提取技术中的若干问题讨论[J].地球与环境,2009,37(4):458-463.
[10]左银虎.环境与植物中硒形态研究进展[J].植物学报,1999,16(4):378-380.
[11]程建中,杨萍,桂仁意.植物硒形态分析的研究综述[J].浙江农林大学学报,2012,29(2):288-295.
[12]Zhu Y Y,Yin X B.Determination of selenium speciation in eggs by LC-UV-AFS[A].In:Banuelos G,Lin Z Q,Yin X,et al..Selenium:Global perspectives of impacts on humans,animals and the environment[C].Beijing:Univeristy of Science and Technology of China Press,2011.
[13]侯遇珠.安徽石台县天然富硒区居民硒摄入量调查与富硒灵芝材料研究[D].合肥:中国科学技术大学,硕士学位论文,2016.
[14]胡海涛,袁林喜,尹雪斌,等.耐硒驯化灵芝菌种的液态发酵中硒的富集特征[J].食品与发酵工业,2012,38(8):7-11.
[15]胡海涛,袁林喜,郑璞,等.4种食用菌硒积累能力比较与硒形态研究[J].中国食用菌,2012,31(3):38-41.
[16]王复龙,彭增起,王蓉蓉,等.动物可食用组织中硒的形态研究进展[J].肉类研究,2011,25(4):57-60.
[17]孙梅,吴强华.农产品中硒形态分析的研究进展[J].理化检验(化学分册),2015,51(11):1621-1626.
[18]Kapolna E,Hillestrom P R,Laursen K H,et al..Effect of foliar application of selenium on its uptake and speciation in carrot[J].Food Chem.,2009,115(4):1357-1363.
[19]Kannamkumarath S S,Wrobel K,Wuilloud R G.Studying the distribution pattern of selenium in nut proteins with information obtained from SEC-UV-ICP-MS and CE-ICP-MS[J].Talanta,2005,66(1):153-159.
[20]富硒农产品GH/T 1135-2017[S].北京:中华全国供销合作社,2017.
[21]张如,樊霆,李淼,等.一株耐硒壶瓶碎米荠内生菌分离、鉴定及其体外硒代谢研究[J].微生物学通报,2017,doi:10.13344/j.microbial.china.170208.
[22]张涛,高愈希,李柏,等.高效液相色谱-等离子体质谱联用方法研究富硒大米中硒的形态[J].分析化学,2008,36(2):206-210.
[23]Gergely V,Kubachka K M,Mounicou S,et al..Selenium speciation in Agaricus bisporus and Lentinula edodes mushroom proteins using multi-dimensional chromatography coupled to inductively coupled plasma mass spectrometry[J].J.Chromatogr.A,2006,1101(1-2):94-102.
[24]李娜,王旭,孙芳芳,等.硒形态分析研究进展[J].湖北农业科学,2011,50(3):437-441.
[25]Hirschfeld T.The hyphenated methods[J].Anal.Chem.,1980,52(2):297-312.
[26]Seubert A.On-line coupling of ion chromatography with ICP-AES and ICP-MS[J].Trends Anal.Chem.,2001,20(6-7):274-287.
[27]尚德荣,秦德元,赵艳芳,等.高压液相色谱-氢化物发生原子荧光光谱(HPLC-HG-AFS)联用技术检测水产品中硒的赋存形态[J].食品安全质量检测学报,2013(6):1847-1852.
[28]肖志明,宋荣,贾铮,等.液相色谱-氢化物发生原子荧光光谱法测定富硒酵母中硒的形态[J].分析化学,2014(9):1314-1319.
[29]曹玉嫔,闫丽珍,黄红丽,等.超声辅助提取结合高效液相色谱-电感耦合等离子体质谱联用技术测定牛蒡和三七中硒形态[J].分析化学,2015(9):1329-1334.
[30]熊珺,覃毅磊,龚亮,等.HPLC-ICP-MS在线联用分析食品中无机硒和硒氨基酸的形态[J].食品工业科技,2017(4):67-72.
[31]张颖,杨清清,宋毅,等.高效液相色谱-电感耦合等离子体质谱联用技术测定富硒食品中无机硒和有机硒的含量[J].中国食品卫生杂志,2017(2):181-185.
[32]刘明钟.原子荧光光谱分析[M].北京:化学工业出版社,2008.
[33]汤杰夫.水稻生长过程中硒-汞互作:来自于土培与野外调查的研究[D].合肥:中国科学技术大学,硕士学位论文,2016.
[34]Szpunar J.Bio-inorganic speciation analysis by hyphenated techniques[J].Analyst,2000,125(5):963-988.
[35]Chan Q,Afton S E,Caruso J A.Selenium speciation profiles in selenite-enriched soybean(Glycine max)by HPLC-ICPMSand ESI-ITMS[J].Metallomics,2010,2(2):147-153.
[36]Floor G H,Igleslas M,Roman R G,et al..Selenium speciation in acidic environmental samples:Application to acid rain-soil interaction at Mount Etna volcano[J].Chemosphere,2011,84(11):1664-1670.
[37]Lenz M,Floor G H,Winkel L H E,et al..Online preconcentration-IC-ICP-MS for selenium quantification and speciation at Ultratraces[J].Environ.Sci.Technol.,2012,46(21):11988-11994.
[38]Sanchez R D,Mellano F,Morales E,et al..A simplified method for inorganic selenium and selenoaminoacids speciation based on HPLC-TR-HG-AFS[J].Talanta,2013,106:298-304.
[39]Chen Z,Akter K F,Rahman M M,et al..The separation of arsenic species in soils and plant tissues by anion-exchange chromatography with inductively coupled mass spectrometry using various mobile phases[J].Microchem.J.,2008,89(1):20-28.
[40]Dasilva E G,Vreola M L R,Zezzi A M A.Speciation analysis of selenium in plankton,Brazil nut and human urine samples by HPLC-ICP-MS[J].Talanta,2013,110:53-57.
[41]程建中.富硒雷竹笋和毛竹笋硒形态研究[D].杭州:浙江农林大学,硕士学位论文,2012.
[42]罗乐,降林华,段宁.HPLC-ICP-MS在工业废渣中硒形态分析中的应用[J].现代化工,2015(11):176-179.
[43]Templrton A,Knowles E.Microbial transformations of minerals and metals:Recent advances in geomicrobiology derived from synchrotron-based X-ray spectroscopy and X-Ray microscopy[J].Ann.Rev.Earth Plant Sci.,2009,37:367-391.
[44]Yoon T H,Johnson S B,Benzera K,et al..In situ characterization of aluminum-containing mineral-microorganism aqueous suspensions using scanning transmission X-ray microscopy[J].Langmuir,2004,20(24):10361-10366.
[45]Toner B,Fakra S,Villalobos M,et al..Spatially resolved characterization of biogenic manganese oxide production within a bacterial biofilm[J].Appl.Environ.Microb.,2005,71(3):1300-1310.
[46]Dynrs J J,Tyliszczak T,Araki T,et al..Speciation and quantitative mapping of metal species in microbial biofilms using scanning transmission X-ray microscopy[J].Environ.Sci.Technol.,2006,40(5):1556-1565.
[47]Ba1uelos G S,Fakra S C,Walse S S,et al..Selenium accumulation,distribution,and speciation in spineless prickly pear cactus:A drought-and salt-tolerant,selenium-enriched nutraceutical fruit crop for biofortified foods[J].Plant Physiol.,2011,155(1):315-327.
[48]Sun G X,Lu X,Williams P N,et al..Distribution and translocation of selenium from soil to grain and its speciation in paddy rice(Oryza sativa L.)[J].Environ.Sci.Technol.,2010,44(17):6706-6711.
[2]吕清慧.食品中砷和硒形态的HPLC-ICP-MS分析方法研究[D].天津:天津科技大学,硕士学位论文,2014.
[3]铁梅,方禹之,孙铁彪,等.HPLC-ICP-MS联用技术在富硒金针菇硒的形态分析中的应用[J].高等学校化学学报,2007,28(4):635-639.
[4]Yuan L X,Zhu Y Y,Lin Z Q,et al..A novel selenocystineaccumulating plant in selenium-mine drainage area in Enshi,China[J].PLo S ONE,2013,8(6):e65615.
[5]Banuelos G S,Stushnoff C,Walse S S,et al..Biofortified,selenium enriched,fruit and cladode from three Opuntia cactus pear cultivars grown on agricultural drainage sediment for use in nutraceutical foods[J].Food Chem.,2012,135(1):9-16.
[6]Banuelos G S,Arroyo I,Pickering I J,et al..Selenium biofortification of broccoli and carrots grown in soil amended with Seenriched hyperaccumulator Stanleya pinnata[J].Food Chem.,2015,166:603-608.
[7]杨红霞.镉形态分析与微区分布的质谱联用技术方法研究及其在印度芥菜耐镉机制中的应用[D].北京:中国地质科学院,博士学位论文,2013.
[8]Kulp T R,Pratt L M.Speciation and weathering of selenium in Upper Cretaceous chalk and shale from South Dakota and Wyoming,USA[J].Geochim.Cosmochimi.Acta,2004,68(18):3687-3701.
[9]李璐,秦海波,苏惠,等.硒连续化学提取技术中的若干问题讨论[J].地球与环境,2009,37(4):458-463.
[10]左银虎.环境与植物中硒形态研究进展[J].植物学报,1999,16(4):378-380.
[11]程建中,杨萍,桂仁意.植物硒形态分析的研究综述[J].浙江农林大学学报,2012,29(2):288-295.
[12]Zhu Y Y,Yin X B.Determination of selenium speciation in eggs by LC-UV-AFS[A].In:Banuelos G,Lin Z Q,Yin X,et al..Selenium:Global perspectives of impacts on humans,animals and the environment[C].Beijing:Univeristy of Science and Technology of China Press,2011.
[13]侯遇珠.安徽石台县天然富硒区居民硒摄入量调查与富硒灵芝材料研究[D].合肥:中国科学技术大学,硕士学位论文,2016.
[14]胡海涛,袁林喜,尹雪斌,等.耐硒驯化灵芝菌种的液态发酵中硒的富集特征[J].食品与发酵工业,2012,38(8):7-11.
[15]胡海涛,袁林喜,郑璞,等.4种食用菌硒积累能力比较与硒形态研究[J].中国食用菌,2012,31(3):38-41.
[16]王复龙,彭增起,王蓉蓉,等.动物可食用组织中硒的形态研究进展[J].肉类研究,2011,25(4):57-60.
[17]孙梅,吴强华.农产品中硒形态分析的研究进展[J].理化检验(化学分册),2015,51(11):1621-1626.
[18]Kapolna E,Hillestrom P R,Laursen K H,et al..Effect of foliar application of selenium on its uptake and speciation in carrot[J].Food Chem.,2009,115(4):1357-1363.
[19]Kannamkumarath S S,Wrobel K,Wuilloud R G.Studying the distribution pattern of selenium in nut proteins with information obtained from SEC-UV-ICP-MS and CE-ICP-MS[J].Talanta,2005,66(1):153-159.
[20]富硒农产品GH/T 1135-2017[S].北京:中华全国供销合作社,2017.
[21]张如,樊霆,李淼,等.一株耐硒壶瓶碎米荠内生菌分离、鉴定及其体外硒代谢研究[J].微生物学通报,2017,doi:10.13344/j.microbial.china.170208.
[22]张涛,高愈希,李柏,等.高效液相色谱-等离子体质谱联用方法研究富硒大米中硒的形态[J].分析化学,2008,36(2):206-210.
[23]Gergely V,Kubachka K M,Mounicou S,et al..Selenium speciation in Agaricus bisporus and Lentinula edodes mushroom proteins using multi-dimensional chromatography coupled to inductively coupled plasma mass spectrometry[J].J.Chromatogr.A,2006,1101(1-2):94-102.
[24]李娜,王旭,孙芳芳,等.硒形态分析研究进展[J].湖北农业科学,2011,50(3):437-441.
[25]Hirschfeld T.The hyphenated methods[J].Anal.Chem.,1980,52(2):297-312.
[26]Seubert A.On-line coupling of ion chromatography with ICP-AES and ICP-MS[J].Trends Anal.Chem.,2001,20(6-7):274-287.
[27]尚德荣,秦德元,赵艳芳,等.高压液相色谱-氢化物发生原子荧光光谱(HPLC-HG-AFS)联用技术检测水产品中硒的赋存形态[J].食品安全质量检测学报,2013(6):1847-1852.
[28]肖志明,宋荣,贾铮,等.液相色谱-氢化物发生原子荧光光谱法测定富硒酵母中硒的形态[J].分析化学,2014(9):1314-1319.
[29]曹玉嫔,闫丽珍,黄红丽,等.超声辅助提取结合高效液相色谱-电感耦合等离子体质谱联用技术测定牛蒡和三七中硒形态[J].分析化学,2015(9):1329-1334.
[30]熊珺,覃毅磊,龚亮,等.HPLC-ICP-MS在线联用分析食品中无机硒和硒氨基酸的形态[J].食品工业科技,2017(4):67-72.
[31]张颖,杨清清,宋毅,等.高效液相色谱-电感耦合等离子体质谱联用技术测定富硒食品中无机硒和有机硒的含量[J].中国食品卫生杂志,2017(2):181-185.
[32]刘明钟.原子荧光光谱分析[M].北京:化学工业出版社,2008.
[33]汤杰夫.水稻生长过程中硒-汞互作:来自于土培与野外调查的研究[D].合肥:中国科学技术大学,硕士学位论文,2016.
[34]Szpunar J.Bio-inorganic speciation analysis by hyphenated techniques[J].Analyst,2000,125(5):963-988.
[35]Chan Q,Afton S E,Caruso J A.Selenium speciation profiles in selenite-enriched soybean(Glycine max)by HPLC-ICPMSand ESI-ITMS[J].Metallomics,2010,2(2):147-153.
[36]Floor G H,Igleslas M,Roman R G,et al..Selenium speciation in acidic environmental samples:Application to acid rain-soil interaction at Mount Etna volcano[J].Chemosphere,2011,84(11):1664-1670.
[37]Lenz M,Floor G H,Winkel L H E,et al..Online preconcentration-IC-ICP-MS for selenium quantification and speciation at Ultratraces[J].Environ.Sci.Technol.,2012,46(21):11988-11994.
[38]Sanchez R D,Mellano F,Morales E,et al..A simplified method for inorganic selenium and selenoaminoacids speciation based on HPLC-TR-HG-AFS[J].Talanta,2013,106:298-304.
[39]Chen Z,Akter K F,Rahman M M,et al..The separation of arsenic species in soils and plant tissues by anion-exchange chromatography with inductively coupled mass spectrometry using various mobile phases[J].Microchem.J.,2008,89(1):20-28.
[40]Dasilva E G,Vreola M L R,Zezzi A M A.Speciation analysis of selenium in plankton,Brazil nut and human urine samples by HPLC-ICP-MS[J].Talanta,2013,110:53-57.
[41]程建中.富硒雷竹笋和毛竹笋硒形态研究[D].杭州:浙江农林大学,硕士学位论文,2012.
[42]罗乐,降林华,段宁.HPLC-ICP-MS在工业废渣中硒形态分析中的应用[J].现代化工,2015(11):176-179.
[43]Templrton A,Knowles E.Microbial transformations of minerals and metals:Recent advances in geomicrobiology derived from synchrotron-based X-ray spectroscopy and X-Ray microscopy[J].Ann.Rev.Earth Plant Sci.,2009,37:367-391.
[44]Yoon T H,Johnson S B,Benzera K,et al..In situ characterization of aluminum-containing mineral-microorganism aqueous suspensions using scanning transmission X-ray microscopy[J].Langmuir,2004,20(24):10361-10366.
[45]Toner B,Fakra S,Villalobos M,et al..Spatially resolved characterization of biogenic manganese oxide production within a bacterial biofilm[J].Appl.Environ.Microb.,2005,71(3):1300-1310.
[46]Dynrs J J,Tyliszczak T,Araki T,et al..Speciation and quantitative mapping of metal species in microbial biofilms using scanning transmission X-ray microscopy[J].Environ.Sci.Technol.,2006,40(5):1556-1565.
[47]Ba1uelos G S,Fakra S C,Walse S S,et al..Selenium accumulation,distribution,and speciation in spineless prickly pear cactus:A drought-and salt-tolerant,selenium-enriched nutraceutical fruit crop for biofortified foods[J].Plant Physiol.,2011,155(1):315-327.
[48]Sun G X,Lu X,Williams P N,et al..Distribution and translocation of selenium from soil to grain and its speciation in paddy rice(Oryza sativa L.)[J].Environ.Sci.Technol.,2010,44(17):6706-6711.