竹节参HPLC指纹图谱的建立及7种成分测定
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摘要
目的建立竹节参Panax japonicus C.A.Mey HPLC指纹图谱,并测定7种核苷类成分的含有量。方法竹节参0.1%氨水提取液的分析采用Venusil MP C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.01%甲酸水,梯度洗脱;体积流量0.8 mL/min;柱温30℃;检测波长254 nm。对结果进行聚类分析和主成分分析。结果 10批样品指纹图谱中有16个共有峰,除S2外相似度均在0.90以上。7种成分在各自范围内线性关系良好(R~2≥0.999 6),平均加样回收率92.74%～98.66%,RSD 2.32%～2.97%。10批样品分为3类。结论该方法简便可靠,重复性好,可用于竹节参药材的质量控制。
AIM To establish the HPLC fingerprints of Panax japonicus C.A.Mey and to determine the contents of seven nucleosides constituents.METHODS The analysis of 0.1% ammonia aqueous extract of P. japonicus was performed on a 30 ℃ thermostastic Venusil MP C_(18) column(250 mm×4.6 mm, 5 μm),with the mobile phase comprising of acetonitrile-0.01% formic acid flowing at 0.8 mL/min in a gradient elution manner, and the detection wavelength was set at 254 nm.The results were analyzed by cluster analysis and principal component analysis.RESULTS There were sixteen common peaks in the fingerprints of ten batches of samples, with the similarities of more than 0.90 except S2.Seven constituents showed good linear relationships within their own ranges(R~2≥0.999 6),the average recoveries were 92.74%～98.66% with the RSDs of 2.32%-2.97%.Ten batches of samples were divided into three types.CONCLUSION This simple, reliable and reproducible method can be used for the quality control of P. japonicus.
AIM To establish the HPLC fingerprints of Panax japonicus C.A.Mey and to determine the contents of seven nucleosides constituents.METHODS The analysis of 0.1% ammonia aqueous extract of P. japonicus was performed on a 30 ℃ thermostastic Venusil MP C_(18) column(250 mm×4.6 mm, 5 μm),with the mobile phase comprising of acetonitrile-0.01% formic acid flowing at 0.8 mL/min in a gradient elution manner, and the detection wavelength was set at 254 nm.The results were analyzed by cluster analysis and principal component analysis.RESULTS There were sixteen common peaks in the fingerprints of ten batches of samples, with the similarities of more than 0.90 except S2.Seven constituents showed good linear relationships within their own ranges(R~2≥0.999 6),the average recoveries were 92.74%～98.66% with the RSDs of 2.32%-2.97%.Ten batches of samples were divided into three types.CONCLUSION This simple, reliable and reproducible method can be used for the quality control of P. japonicus.
引文
[1] 国家药典委员会.中华人民共和国药典:2015年版一部[S].北京:中国医药科技出版社,2015:138-139.
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[10] 吴琳琳,姚文丽,罗奕,等.10个采收期青钱柳HPLC指纹图谱建立及4种成分测定[J].中成药,2017,39(2):347-352.
[11] 吕娜,王钧篪,陈丽华,等.康复新液HPLC指纹图谱建立及6种成分测定[J].中成药,2018,40(3):613-617.
[12] 曾慧婷,沙秀秀,宿树兰,等.不同产地丹参茎叶UPLC指纹图谱与化学模式识别研究[J].中草药,2017,48(4):767-772.
[13] Gao F,Xu Z,Wang W,et al.A comprehensive strategy using chromatographic profiles combined with chemometric methods:Application to quality control of Polygonum cuspidatum Sieb.et Zucc[J].J Chromatogr A,2016,1466:67-75.
[14] 周恒,曹依敏,苗水,等.HPLC法测定沪地龙中7个核苷类成分的含量[J].药物分析杂志,2018,38(1):97-103.
[15] 毛坤军,李富强,白钢钢,等.HPLC同时测定虎力散片剂中10个核苷和碱基类成分含量[J].药物分析杂志,2014,34(11):1959-1963.
[2] 武秋爽,陈平,张庆文.竹节参化学成分、药理活性及分析方法研究进展[J].亚太传统医药,2016,12(6):46-54.
[3] 吴兵,陈新,张长春,等.竹节参化学成分研究[J].天然产物研究与开发,2012,24(8):1051-1054,1093.
[4] 贾放,陈平,王如锋,等.竹节参药材不同部位皂苷成分的分析研究[J].时珍国医国药,2012,23(7):1682-1684.
[5] 张杰,李春艳,李劲平,等.蒽酮硫酸法与苯酚硫酸法测定竹节参多糖含量的比较研究[J].中南药学,2012,10(6):421-424.
[6] 林先容,马生芹,章倩倩,等.竹节参中挥发性成分分析[J].中国保健营养,2017,27(13):14,16.
[7] 丁兴杰,熊亮,周勤梅,等.天然核苷类成分的化学结构和药理活性研究进展[J].成都中医药大学学报,2018,41(2):102-108.
[8] 张元杰,钱正明,陈肖家,等.HPLC法同时测定补益中药中尿苷、腺嘌呤、鸟苷和腺苷的含量[J].药物分析杂志,2010,30(1):33-36.
[9] 刘玉峰,杨秀伟.款冬花药材的HPLC化学成分指纹图谱研究[J].药学学报,2009,44(5):510-514.
[10] 吴琳琳,姚文丽,罗奕,等.10个采收期青钱柳HPLC指纹图谱建立及4种成分测定[J].中成药,2017,39(2):347-352.
[11] 吕娜,王钧篪,陈丽华,等.康复新液HPLC指纹图谱建立及6种成分测定[J].中成药,2018,40(3):613-617.
[12] 曾慧婷,沙秀秀,宿树兰,等.不同产地丹参茎叶UPLC指纹图谱与化学模式识别研究[J].中草药,2017,48(4):767-772.
[13] Gao F,Xu Z,Wang W,et al.A comprehensive strategy using chromatographic profiles combined with chemometric methods:Application to quality control of Polygonum cuspidatum Sieb.et Zucc[J].J Chromatogr A,2016,1466:67-75.
[14] 周恒,曹依敏,苗水,等.HPLC法测定沪地龙中7个核苷类成分的含量[J].药物分析杂志,2018,38(1):97-103.
[15] 毛坤军,李富强,白钢钢,等.HPLC同时测定虎力散片剂中10个核苷和碱基类成分含量[J].药物分析杂志,2014,34(11):1959-1963.