抗菌治疗对兔颊VX-2鳞癌感染性微环境中树突状细胞的影响
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摘要
目的:探索抗菌治疗对兔颊VX-2鳞癌感染性微环境中树突状细胞(dendritic cells,DCs)成熟及功能的影响。方法:瘤粒植入形成兔颊VX-2鳞癌后,附加机械创伤及高糖饮食获得该鳞癌的炎症模型,再分为3组(n=6),A组:颊癌炎症模型使用抗生素治疗3 d;B组:颊癌炎症模型以生理盐水代替抗生素对照;C组:单纯颊癌不做处理。3 d后收集各组肿瘤标本,制成匀浆,取上清液与正常兔外周血单核细胞共培养,流式细胞仪检测DCs表面分子HLA-DR、CD83、CD86的表达,混合淋巴细胞反应检测DCs刺激T细胞增殖的能力。结果:HLA-DR、CD83、CD86阳性率及刺激指数(stimulate index,SI)由高到低均分别为:C组>A组>B组(P<0.05)。结论:抗菌治疗有助于感染兔颊VX-2鳞癌微环境中树突状细胞的成熟及功能恢复。
Objective: To study the effects of antimicrobial therapy on the maturation and function of dendritic cells( DCs) in the infected microenvironment of rabbit buccal VX-2 squamous cell carcinoma. Methods: The inflammatory models were obtained by mechanical trauma and high sugar diet on the basis of rabbit buccal VX-2 squamous cell carcinoma models which were established by particle implantation of the tumor tissue. The model rabbits were divided into 3 groups( n = 6). In group A the rabbits with buccal VX-2 squamous cell carcinoma and local inflammation were given antibiotics by gavage and intramuscular injection for 3 consecutive days; the rabbits in group B with buccal VX-2 squamous cell carcinoma and local inflammation were given normal saline by gavage and intramuscular injection; the rabbits in group C with tumor and without inflammation were given normal saline by gavage and intramuscular injection. The tumor specimens were collected 3 days after treatment,and made into tissue homogenate,supernatant was collected after centrifugation. Normal rabbit peripheral blood mononuclear cells were separated and co-cultured with the supernatant obtained from the 3 groups respectively. Expressions of DCs surface markers HLA-DR,CD83 and CD86 were detected by flow cytometry. the function of DCs was tested by mixed lymphocyte reaction. Results: The positive rate of HLA-DR,CD83,CD86 and stimulate index were group C > group A > group B( P < 0. 05). Conclusion: Antimicrobial therapy can promote the maturation and function of DCs in the infected microenvironment of rabbit buccal VX-2 squamous cell carcinoma.
Objective: To study the effects of antimicrobial therapy on the maturation and function of dendritic cells( DCs) in the infected microenvironment of rabbit buccal VX-2 squamous cell carcinoma. Methods: The inflammatory models were obtained by mechanical trauma and high sugar diet on the basis of rabbit buccal VX-2 squamous cell carcinoma models which were established by particle implantation of the tumor tissue. The model rabbits were divided into 3 groups( n = 6). In group A the rabbits with buccal VX-2 squamous cell carcinoma and local inflammation were given antibiotics by gavage and intramuscular injection for 3 consecutive days; the rabbits in group B with buccal VX-2 squamous cell carcinoma and local inflammation were given normal saline by gavage and intramuscular injection; the rabbits in group C with tumor and without inflammation were given normal saline by gavage and intramuscular injection. The tumor specimens were collected 3 days after treatment,and made into tissue homogenate,supernatant was collected after centrifugation. Normal rabbit peripheral blood mononuclear cells were separated and co-cultured with the supernatant obtained from the 3 groups respectively. Expressions of DCs surface markers HLA-DR,CD83 and CD86 were detected by flow cytometry. the function of DCs was tested by mixed lymphocyte reaction. Results: The positive rate of HLA-DR,CD83,CD86 and stimulate index were group C > group A > group B( P < 0. 05). Conclusion: Antimicrobial therapy can promote the maturation and function of DCs in the infected microenvironment of rabbit buccal VX-2 squamous cell carcinoma.
引文
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[2]Hussain SP,Harris CC.Inflammation and cancer:An ancient link with novel potentials[J].Int J Cancer,2007,121(11):2373-2380.
[3]张林,张霓霓,黄桂林,等.抗感染对伴炎症兔颊VX2鳞状细胞癌组织中树突状细胞表面分子HLA-DR和CD86表达的影响[J].华西口腔医学杂志,2015,33(2):141-144.
[4]沈政洁,程海波,沈卫星,等.肿瘤炎性微环境与“癌毒”病机相关性探讨[J].北京中医药大学学报,2015,38(1):14-17.
[5]罗颖蓝,鲍依稀.树突状细胞疫苗在肝癌免疫治疗中的研究进展[J].国际检验医学杂志,2010,31(9):971-973.
[6]Ostrand-Rosenberg S,Sinha P.Myeloid-derived suppressor cells:Linking inflammation and cancer[J].J Immunol,2009,182(8):4499-4506.
[7]Palucka K,Ueno H,Roberts L,et al.Dendritic cell subsets as vectors and targets for improved cancer therapy[J].Curr Top Microbiol Immunol,2011,344:173-192.
[8]Ma Y,Shurin GV,Peiyuan Z,et al.Dendritic cells in the cancer microenvironment[J].J Cancer,2013,4(1):36-44.
[9]Lin A,Schildknecht A,Nguyen LT,et al.Dendritic cells integrate signals from the tumor microenvironment to modulate immunity and tumor growth[J].Immunol Lett,2010,127(2):77-84.
[10]孙桂芝,周同,张玉梅,等.白细胞介素-10对人树突状细胞表型及致炎细胞因子分泌的影响[J].上海第二医科大学学报,2004,24(7):510-513.
[11]何浩,宋文刚.调节性DC与调节性T细胞的相互作用[J].免疫学杂志,2010,26(6):551-554.
[12]Gottfried E,Kreutz M,Mackensen A.Tumor-induced modulation of dendritic cell function[J].Cytokine Growth Factor Rev,2008,19(1):65-77.