紫杉醇脂质体与紫杉醇不同途径灌注治疗兔舌癌的疗效研究
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摘要
目的:研究经兔舌动脉、耳缘静脉分别灌注紫杉醇脂质体或紫杉醇治疗兔VX-2舌癌的疗效。方法:建立兔VX-2舌癌模型,随机分为6组(n=4)。其中,舌动脉灌注3组,分别灌注紫杉醇脂质体,紫杉醇,5%葡萄糖溶液;耳缘静脉灌注3组,用药同上。治疗后7 d,采用体积测量、流式细胞术、免疫组织化学对舌癌病灶的治疗反应、肿瘤细胞凋亡、P53蛋白表达进行检测。数据经SPSS 20.0统计软件分析。结果:舌动脉灌注紫杉醇脂质体组有效率明显优于其他各组(P<0.05),细胞凋亡率明显高于其它各组(P<0.05),P53蛋白表达明显低于其它各组(P<0.05)。结论:舌动脉灌注紫杉醇脂质体进行兔VX-2舌癌治疗效果最佳。
Objective: To study the effects of the treatment for rabbit VX-2 tongue cancer with paclitaxel or paclitaxel liposome infusion through rabbit lingual artery or ear vein infusion. Methods: 24 rabbits with VX-2 tongue cancer were divided into 6 groups( n =40) randomly. The rabits in 3 groups were respectively treated by intra-arterial infusion with paclitaxel,paclitaxel liposome and 5% glucose,and those in other 3 groups by ear vein infusion with the same materials. After 7 days of treatment,the lesion volumes were measured for response evaluation,cells apotosis in the cancer tissure was detected by flowcytometry,P53 protein expression was examined by immunohistochemical staining,respectively. The data was analyzed by SPSS 20. 0 software package. Results: The response rate and apotosis of the tumor cells in intra-arterial infusion with paclitaxel liposome group were higher than those in the other groups( P< 0. 05),P53 protein expression was lower than that of the other groups( P < 0. 05). Conclusion: Intra-arterial infusion through tongue artery with paclitaxel liposome is more effective than the other methods in the treatment of rabbit VX-2 tongue cancer.
Objective: To study the effects of the treatment for rabbit VX-2 tongue cancer with paclitaxel or paclitaxel liposome infusion through rabbit lingual artery or ear vein infusion. Methods: 24 rabbits with VX-2 tongue cancer were divided into 6 groups( n =40) randomly. The rabits in 3 groups were respectively treated by intra-arterial infusion with paclitaxel,paclitaxel liposome and 5% glucose,and those in other 3 groups by ear vein infusion with the same materials. After 7 days of treatment,the lesion volumes were measured for response evaluation,cells apotosis in the cancer tissure was detected by flowcytometry,P53 protein expression was examined by immunohistochemical staining,respectively. The data was analyzed by SPSS 20. 0 software package. Results: The response rate and apotosis of the tumor cells in intra-arterial infusion with paclitaxel liposome group were higher than those in the other groups( P< 0. 05),P53 protein expression was lower than that of the other groups( P < 0. 05). Conclusion: Intra-arterial infusion through tongue artery with paclitaxel liposome is more effective than the other methods in the treatment of rabbit VX-2 tongue cancer.
引文
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[2]胡小华,黄桂林,张霓霓,等.口腔癌持续性动脉灌注新辅助化疗的疗效观察[J].中国肿瘤临床,2012,39(18):1382-1385.
[3]易杰,黄桂林,乔薪瑾,等.5-Fu经不同给药途径持续灌注后舌及下颌下淋巴结内浓度比较[J].实用口腔医学杂志,2014,30(1):34-37.
[4]苏薇,张晟,李春艳,等.紫杉醇脂质体和多西紫杉醇在乳腺癌新辅助化疗中的应用效果及安全性[J].中华肿瘤杂志,2015,37(5):379-382.
[5]童树友,张娟,朱坤鹍,等.兔口腔Vx-2肿瘤模型的建立[J].安徽医科大学学报,2008,43(2):151-153.
[6]Yokoyama J,Ito S,Ohba S,et al.A novel approach to translymphatic chemotherapy targeting sentinel lymph nodes of patients with oral cancer using intraarterial chemotherapypreliminary study[J].Head Neck Oncol,2011,3:42.
[7]Lima-Tenório MK,Gómez Pineda EA,Ahmad NM,et al.Magnetic nanoparticles:In vivo cancer diagnosis and therapy[J].Int J Pharm,2015,493(1-2):313-327.
[8]Baek S,Singh RK,Khanal D,et al.Smart multifunctional drug delivery towards anticancer therapy harmonized in mesoporous nanoparticles[J].Nanoscale,2015,7(34):14191-14216.
[9]Sharpless NE,De Pinho RA.p53:good cop/bad cop[J].Cell,2002,110(1):9-12.
[10]Vikhanskaya F,Lee MK,Mazzoletti M,et al.Cancer derived p53 mutants suppress p53-target gene expression-potential mechanism for gain of function of mutant p53[J].Nucleic Acids Res,2007,35(6):2093-2104.
[11]Sano D,Xie TX,Ow TJ,et al.Disruptive TP53 mutation is associated with aggressive disease characteristics in an orthotopic murine model of oral tongue cancer[J].Clin Cancer Res,2011,17(21):6658-6670.