火棘多糖铁(Ⅲ)复合物中铁含量检测方法的建立
|
摘要
为了优化建立火棘多糖铁(Ⅲ)复合物(PPC)中铁含量检测的方法。考察了邻菲啰啉溶液浓度(w/w,%)及用量(mL)、抗坏血酸溶液浓度(w/w,%)及用量(mL)、反应时间(h)及温度(℃)等因素对PPC中铁含量测定的影响。在单因素实验的基础上,运用响应面软件进一步优化PPC的检测条件。结果表明,PPC中铁含量的最佳检测条件为:向1.0 mL适宜浓度的PPC溶液中,依次加入1.5 mL 10%的抗坏血酸溶液及3.0 mL 0.1%的邻菲啰啉溶液,于45℃水浴2.0 h后,冰水快速冷却,在室温下于510 nm波长处测其吸光度。该方法具有良好的重复性及重现性,回收率达99.93%。此方法也适合推广于其他植物多糖铁复合物中铁含量的测定。
In order to get the optimum conditions for determination of iron in Pyracantha polysaccharide-Fe(III) complex(PPC).The effects of O-phenanthroline concentration(w/w,%) and its amount(mL),ascorbic acid concentration(w/w,%) and its amount(mL),reaction time(h) as well as its temperature(℃) on the determination of iron in PPC were investigated.On the basis of single factor experiment,response surface software was used to further optimize the detection conditions of PPC.The results showed that the optimum conditions for the determination of iron content in PPC were as follows:1.5 mL 10% ascorbic acid and 3.0 mL 0.1% O-phenanthroline solution were added successively to the PPC solution of 1.0 mL of suitable concentration,and the absorbance was measured at 510 nm at room temperature after a water bath at 45 ℃ for 2.0 h.The developed method had good reproducibility and reproducibility with a recovery rate of 99.93%,which can be applied to detect iron content in other plant polysaccharide-iron complexes.
In order to get the optimum conditions for determination of iron in Pyracantha polysaccharide-Fe(III) complex(PPC).The effects of O-phenanthroline concentration(w/w,%) and its amount(mL),ascorbic acid concentration(w/w,%) and its amount(mL),reaction time(h) as well as its temperature(℃) on the determination of iron in PPC were investigated.On the basis of single factor experiment,response surface software was used to further optimize the detection conditions of PPC.The results showed that the optimum conditions for the determination of iron content in PPC were as follows:1.5 mL 10% ascorbic acid and 3.0 mL 0.1% O-phenanthroline solution were added successively to the PPC solution of 1.0 mL of suitable concentration,and the absorbance was measured at 510 nm at room temperature after a water bath at 45 ℃ for 2.0 h.The developed method had good reproducibility and reproducibility with a recovery rate of 99.93%,which can be applied to detect iron content in other plant polysaccharide-iron complexes.
引文
1 Li SJ.Pyracantha fortuneana resources chemistry and its antioxidant effect[D].Wuhan:Huazhong University of Science and Technology(华中科技大学),2011.
2 Jiang LH,Xiong YF,Li X,et al.Advances on research of effective constituents of wild Pyracantha fortuneana[J].Chin WiId Plant Res(中国野生植物资源),2007,26(2):8-10.
3 Huang R,Fu XH.Advances on research of functional composition of Pyracantha fortuneana[J].Chin WiId Plant Res(中国野生植物资源),2014,33(5):37-41.
4 Wang XH,Bai HQ,Wulangerile.Progress in the studies of polysaccharide metal complex[J].J Inner Mongolia Univ Nation(内蒙古民族大学学报),2014,29:516-519.
5 Goodnough LT,Nemeth E,Ganz T.Detection,evaluation,and management of iron-restricted erythropoiesis[J].Blood,2010,116:4754-4761.
6 Powers JM,Daniel CL,McCavit TL,et al.Deficiencies in the management of iron deficiency anemia during childhood[J].Pediatric Blood & Cancer,2016,63:743-745.
7 Wang FH,Zhao W,Chen JF,et al.Research progress in iron supplement[J].Prog in Pharm Sci(药学进展),2016,9:680-688.
8 Macdougall IC.Evolution of IV iron compounds over the last century[J].J Ren Care,2009,35(S2):8-13.
9 Mao K,Ma YX,Pan CH,et al.Research progress in new types of intravenous iron[J].Chin J New Drug(中国新药杂志),2015,6:659-663.
10 Wang BQ,Xu ZP,Lu XH.Research progress of polysaccharide-iron complex[J].J Anhui Agric Sci(安徽农业科学),2007,23:44-46.
11 Dong YR,Cheng GT.Research progress of polysaccharide-iron compound[J].J Food Safe Qua(食品安全质量检测学报),2015,6:2890-2895.
12 Chen GX,Xian H.Research progress of plant polysaccharide iron complex[J].Chongqing J Res Chin Drugs Herbs(重庆中草药研究),2009,2:35-42.
13 Zhao P,Zhang TT,Zhang LH,et al.The response surface method optimization pour egg leaves five plus polysaccharide(Ⅲ) synthetic technology conditions of iron[J].Chin Tradit Pat Med(中成药),2014,11:2418-2420.
14 Wang F,Ma WL,Hu ZX,et al.Optimization of Gynostemma polysaccharide-Fe complex of preparation with response surface methodology[J].Food Ind(食品工业),2014,35(5):81-86.
15 Ren GM,Guo X,Cheng ZX.Optimization of synthesis of lentinan-iron(Ⅲ)complex by response surface methodology[J] Sci Technol Food Ind(食品工业科技),2014,4:251-255.
16 Zhao P,Li WH,Li L,et al.Synthesis and adsorption kinetics of Limonium bicolor polysaccharide-iron(Ⅲ) complex[J].Chem Eng(化学工程),2014,11:6-9.
17 Dong YR,Wang Y,Zhao LY,et al.Separation,purification of polysaccharides and preparation of polysaccharide-iron complex from Flammulina velutipes[J].Food Sci Technol(食品科技),2017,1:208-213.
18 Zhang XL,Tian HW,Wang J.Determination of iron content in iron polysaccharide complex by UV-VIS[J].J Pharm Res(药学研究),2015,11:645-647.
19 Li YX,You ZH,Zhu YX.Synthesis of lily polysaccharide-Fe(Ⅲ) complex and determination of Fe(Ⅲ)[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2010,7:47-49.
20 Wulangerile,Li YT,Bai HQ,et al.Purified preparation of Polygonatum odoratum(Mill.) Druce polysaccharide-Fe complex and assay of Fe[J].J Pharm Anal(药物分析杂志),2012,4:571-574.
2 Jiang LH,Xiong YF,Li X,et al.Advances on research of effective constituents of wild Pyracantha fortuneana[J].Chin WiId Plant Res(中国野生植物资源),2007,26(2):8-10.
3 Huang R,Fu XH.Advances on research of functional composition of Pyracantha fortuneana[J].Chin WiId Plant Res(中国野生植物资源),2014,33(5):37-41.
4 Wang XH,Bai HQ,Wulangerile.Progress in the studies of polysaccharide metal complex[J].J Inner Mongolia Univ Nation(内蒙古民族大学学报),2014,29:516-519.
5 Goodnough LT,Nemeth E,Ganz T.Detection,evaluation,and management of iron-restricted erythropoiesis[J].Blood,2010,116:4754-4761.
6 Powers JM,Daniel CL,McCavit TL,et al.Deficiencies in the management of iron deficiency anemia during childhood[J].Pediatric Blood & Cancer,2016,63:743-745.
7 Wang FH,Zhao W,Chen JF,et al.Research progress in iron supplement[J].Prog in Pharm Sci(药学进展),2016,9:680-688.
8 Macdougall IC.Evolution of IV iron compounds over the last century[J].J Ren Care,2009,35(S2):8-13.
9 Mao K,Ma YX,Pan CH,et al.Research progress in new types of intravenous iron[J].Chin J New Drug(中国新药杂志),2015,6:659-663.
10 Wang BQ,Xu ZP,Lu XH.Research progress of polysaccharide-iron complex[J].J Anhui Agric Sci(安徽农业科学),2007,23:44-46.
11 Dong YR,Cheng GT.Research progress of polysaccharide-iron compound[J].J Food Safe Qua(食品安全质量检测学报),2015,6:2890-2895.
12 Chen GX,Xian H.Research progress of plant polysaccharide iron complex[J].Chongqing J Res Chin Drugs Herbs(重庆中草药研究),2009,2:35-42.
13 Zhao P,Zhang TT,Zhang LH,et al.The response surface method optimization pour egg leaves five plus polysaccharide(Ⅲ) synthetic technology conditions of iron[J].Chin Tradit Pat Med(中成药),2014,11:2418-2420.
14 Wang F,Ma WL,Hu ZX,et al.Optimization of Gynostemma polysaccharide-Fe complex of preparation with response surface methodology[J].Food Ind(食品工业),2014,35(5):81-86.
15 Ren GM,Guo X,Cheng ZX.Optimization of synthesis of lentinan-iron(Ⅲ)complex by response surface methodology[J] Sci Technol Food Ind(食品工业科技),2014,4:251-255.
16 Zhao P,Li WH,Li L,et al.Synthesis and adsorption kinetics of Limonium bicolor polysaccharide-iron(Ⅲ) complex[J].Chem Eng(化学工程),2014,11:6-9.
17 Dong YR,Wang Y,Zhao LY,et al.Separation,purification of polysaccharides and preparation of polysaccharide-iron complex from Flammulina velutipes[J].Food Sci Technol(食品科技),2017,1:208-213.
18 Zhang XL,Tian HW,Wang J.Determination of iron content in iron polysaccharide complex by UV-VIS[J].J Pharm Res(药学研究),2015,11:645-647.
19 Li YX,You ZH,Zhu YX.Synthesis of lily polysaccharide-Fe(Ⅲ) complex and determination of Fe(Ⅲ)[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2010,7:47-49.
20 Wulangerile,Li YT,Bai HQ,et al.Purified preparation of Polygonatum odoratum(Mill.) Druce polysaccharide-Fe complex and assay of Fe[J].J Pharm Anal(药物分析杂志),2012,4:571-574.