两色金鸡菊总黄酮部位多指标成分含量测定
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摘要
目的:采用超高效液相色谱-二极管阵列检测器(UPLC-DAD)建立两色金鸡菊Coreopsis tinctoria Nutt.中R/S-异奥卡宁-7-O-β-D-葡萄糖苷、对羟基桂皮酸甲酯-4-O-β-D-吡喃葡萄糖苷、槲皮万寿菊素-7-O-β-D-葡萄糖苷、异奥卡宁、槲皮素-7-O-葡萄糖苷、马里苷、金鸡菊苷、奥卡宁、圣草酚、紫铆花素10种成分含量测定方法,为两色金鸡菊药理研究及药效物质基础研究提供参考。方法:采用Waters ACQUITY UPLC HSS T3色谱柱(100 mm×2.1 mm,1.8μm);流动相为0.1%甲酸水和乙腈;流速为0.3 mL·min~(-1);柱温为30℃;紫外检测波长为285 nm与378 nm;进样量为3μL。结果:建立两色金鸡菊总黄酮部位超高效液相方法,方法学考察结果符合《中华人民共和国药典》相关要求,测定了10种成分含量。结论:建立的两色金鸡菊总黄酮部位10种化学成分含量测定方法稳定、重复性好、精密度高,可为两色金鸡菊总黄酮部位的质量控制及药理研究提供参考。
Objective:To establish a UPLC-DAD method for content determination of(R/S)-flavanomarein,linocinnamarin,quercetagetin-7-O-β-D-glucoside,isookanin,quercetin-7-O-glucoside,marein,butein-4-O-β-D-glucopyranoside,okanin,eriodictyol and butein from Coreopsis tinctoria flavonoids fraction and provide a reference for the pharmacological research and of C.tinctoria.Method:Waters ACQUITY UPLC HSS T3 column(100 mm×2.1 mm,1.8 μm)was applied with mobile phase of 0.1% formic acid and acetonitrile.The flow rate was 0.3 mL·min~(-1) and column temperature was 30 ℃,and UV detection wavelength was 285 nm and 378 nm.Results:The UPLC method for the flavonoids fraction of C.tinctoria was established.The methodological study conformed to the requirements of Pharmacopoeia of People′s Republic of China.Conclusion:The method for content determination of 10 chemical constituents in the flavonoids fractions of C.tinctoria was verified to be stable,reproducible and high-precision,which can provide a basis for quality control and pharmacological research of the flavonoids fractions of C.tinctoria.
Objective:To establish a UPLC-DAD method for content determination of(R/S)-flavanomarein,linocinnamarin,quercetagetin-7-O-β-D-glucoside,isookanin,quercetin-7-O-glucoside,marein,butein-4-O-β-D-glucopyranoside,okanin,eriodictyol and butein from Coreopsis tinctoria flavonoids fraction and provide a reference for the pharmacological research and of C.tinctoria.Method:Waters ACQUITY UPLC HSS T3 column(100 mm×2.1 mm,1.8 μm)was applied with mobile phase of 0.1% formic acid and acetonitrile.The flow rate was 0.3 mL·min~(-1) and column temperature was 30 ℃,and UV detection wavelength was 285 nm and 378 nm.Results:The UPLC method for the flavonoids fraction of C.tinctoria was established.The methodological study conformed to the requirements of Pharmacopoeia of People′s Republic of China.Conclusion:The method for content determination of 10 chemical constituents in the flavonoids fractions of C.tinctoria was verified to be stable,reproducible and high-precision,which can provide a basis for quality control and pharmacological research of the flavonoids fractions of C.tinctoria.
引文
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[11] 刘谢英,张玉珊,姚新成.两色金鸡菊HPLC特征图谱的建立及多指标成分含量测定[J].中药材,2018,41(5):1129-1133.
[12] JIANG B,XIAO P G,LE L.Transcriptome analysis reveals the mechanism of the effect of flower tea Coreopsis tinctoria on hepatic insulin resistance[J].Food Funct,2018,9(11):5607-5620
[13] 张雨洁,兰怡,李卉,等.两色金鸡菊乙酸乙酯提取物对STZ诱导的糖尿病SD大鼠的影响[J].中国药理学通报,2015,31(10):1439-1443.
[14] 姚蓝,蒋洁,王靖宣,等.两色金鸡菊对糖尿病大鼠肾脏纤维化RhoA/ROCK/Nox4信号通路的影响[J].中国中医药信息杂志,2019,26(1):57-62.
[15] 张瑞,李新霞,毛新民,等.HPLC法测定两色金鸡菊提取物中Flavanomarein和 Marein的含量[J].安徽农业科学,2015,43(6):73-74.
[16] JIANG B,LE L,ZHAI W,et al.Protective effects of marein on high glucose-induced glucose metabolic disorder in HepG2 cells[J].Phytomedicine,2016,23(9):891-900.
[17] JIANG B,LE L,LIU H,et al.Marein protects against methylglyoxal-induced apoptosis by activating the AMPK pathway in PC12 cells[J].Free Radic Res,2016,50(11):1173-1187.
[18] HOU Y,LI G,WANG J,et al.Okanin,effective constituent of the flower tea Coreopsis tinctoria,attenuates LPS-induced microglial activation through inhibition of the TLR4/NF-κB signaling pathways[J].Sci Rep,2017,7:1-13.
[19] PITSCHMANN A,ZEHL M,HEISS E,et al.Quantitation of phenylpropanoids and iridoids in insulin-sensitising extracts of Leonurus sibiricus L.(Lamiaceae):PCA[J].Phytochem Anal,2016,27(1):23-31.
[2] GUO L,ZHANG W,LI S,et al.Chemical and nutraceutical properties of Coreopsis tinctoria[J].J Funct Foods,2015,13:11-20.
[3] 陈安新.中国雪菊[M].乌鲁木齐:新疆美术摄影出版社,2013,12-15.
[4] Christina Zlaru,Claudia C Cri?an,Ioan Clinescu,et al.Polyphenols in Coreopsis tinctoria Nutt.fruits and the plant extracts antioxidant capacity evaluation[J].Cent Eur J Chem,2014,12(8):858-867.
[5] 古扎力努尔·艾尔肯,李新霞,毛新民,等.新疆两色金鸡菊中绿原酸与总黄酮含量测定及指纹图谱的建立[J].西北药学杂志,2013,28(3):248-251.
[6] DIAS T,LIU B,JONES P,et al.Cytoprotective effect of Coreopsis tinctoria extracts and flavonoids on tBHP and cytokine-induced cell injury in pancreatic MIN6 cells[J].J Ethnopharmacol,2012,139(2):485-492.
[7] LI Y,CHEN X,XUE J,et al.Flavonoids furom Coreopsis tinctoria adjust lipid metabolism in hyperlipidemia animals by down-regulating adipose differentiation-related protein[J].Lipids Heal Th Dis,2014,13(1):193-200.
[8] Dias T,Maria Rosário Bronze,Houghton P J,et al.The flavonoid-rich fraction of Coreopsis tinctoria promotes glucose tolerance regain through pancreatic function recovery in streptozotocin-induced glucose-intolerant rats[J].J Ethnopharmacol,2010,132(2):483-490.
[9] DENG Y,LAM S C,ZHAO J,et al.Quantitative analysis of flavonoids and phenolic acid in Coreopsis tinctoria Nutt.by capillary zone electrophoresis[J].Electrophoresis,2017,38(20):2654-2661.
[10] YANG Y J,SUN X G,LIU J J,et al.Quantitative and Qualitative Analysis of Flavonoids and Phenolic Acids in Snow Chrysanthemum(Coreopsis tinctoria Nutt.)by HPLC-DAD and UPLC-ESI-QTOF-MS[J].Molecules,2016,21(10):1307-1325.
[11] 刘谢英,张玉珊,姚新成.两色金鸡菊HPLC特征图谱的建立及多指标成分含量测定[J].中药材,2018,41(5):1129-1133.
[12] JIANG B,XIAO P G,LE L.Transcriptome analysis reveals the mechanism of the effect of flower tea Coreopsis tinctoria on hepatic insulin resistance[J].Food Funct,2018,9(11):5607-5620
[13] 张雨洁,兰怡,李卉,等.两色金鸡菊乙酸乙酯提取物对STZ诱导的糖尿病SD大鼠的影响[J].中国药理学通报,2015,31(10):1439-1443.
[14] 姚蓝,蒋洁,王靖宣,等.两色金鸡菊对糖尿病大鼠肾脏纤维化RhoA/ROCK/Nox4信号通路的影响[J].中国中医药信息杂志,2019,26(1):57-62.
[15] 张瑞,李新霞,毛新民,等.HPLC法测定两色金鸡菊提取物中Flavanomarein和 Marein的含量[J].安徽农业科学,2015,43(6):73-74.
[16] JIANG B,LE L,ZHAI W,et al.Protective effects of marein on high glucose-induced glucose metabolic disorder in HepG2 cells[J].Phytomedicine,2016,23(9):891-900.
[17] JIANG B,LE L,LIU H,et al.Marein protects against methylglyoxal-induced apoptosis by activating the AMPK pathway in PC12 cells[J].Free Radic Res,2016,50(11):1173-1187.
[18] HOU Y,LI G,WANG J,et al.Okanin,effective constituent of the flower tea Coreopsis tinctoria,attenuates LPS-induced microglial activation through inhibition of the TLR4/NF-κB signaling pathways[J].Sci Rep,2017,7:1-13.
[19] PITSCHMANN A,ZEHL M,HEISS E,et al.Quantitation of phenylpropanoids and iridoids in insulin-sensitising extracts of Leonurus sibiricus L.(Lamiaceae):PCA[J].Phytochem Anal,2016,27(1):23-31.