摘要
目的研究白藜芦醇(RSV)防护糖尿病早期视损伤的分子机制。方法通过建立视网膜muller细胞(RMCs)体外高糖模型,采用免疫荧光双标、qRT-PCR、western blot、Annexin V/PI流式细胞技术检测高糖和RSV对微小RNA-29b(miR-29b)、特异蛋白1(SP1)表达及RMCs凋亡的影响。结果高糖培养后,miR-29b和SP1表达明显改变,RMCs凋亡率明显增加,RSV干预可明显抑制高糖诱导的miR-29b和SP1异常表达和RMCs凋亡;通过预先在RMCs中转染miR-29b类似物和miR-29b抑制剂研究发现,RSV抑制凋亡作用受明显影响(P<0.05)。结论 RSV通过调节miR-29b表达发挥抗糖尿病早期视损伤作用。
Objective To investigate the molecular mechanisms of protecting early diabetic retinopathy by resvertrol( RSV).Methods The effects of RSV on expressions of miRNA-29b( miR-29b) and specificity protein 1( SP1) as well as apoptosis of retinal muller cells( RMCs) were investigated through development of rat RMCs hyperglucose model in vito,and mothods of immunofluorescence,qRT-PCR,western blot and Annexin V/PI flow-cytometry.Results High glucose induced down-regulation of miR-29 b and upregulation of SP1 and increased the apoptosis of RMCs.The effects could be reversed by RSV in vitro.Moreover,the anti-apoptosis effect of RSV could be reversed by transfection of miR-29 b analogues to RMCs and miR-29 b inhibitor( P < 0. 05).Conclusion Resveratrol can inhibit diabetic retinopathy in early stage by regulation of miR-29 b expression.
引文
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