摘要
目的:观察贝母提取物(FE)对异丙肾上腺素(Iso)致小鼠心肌纤维化的作用。方法:昆明种小鼠40只,雌雄兼用,随机分为4组(n=10):对照组、模型组、FE 900 mg/kg、300 mg/kg剂量组,每只i.h Iso1.5 mg/kg(0.3 ml/d)造模,持续14 d;高、低给药组造模同时分别0.9 ml/d,0.4 ml/d灌胃FE,对照组s.c.等量生理盐水,连续30 d。实验结束,测定小鼠心重指数,血清中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量,HE和Masson染色分析心肌组织病理改变,免疫组化法检测心肌转化生长因子β1(TGF-β1)表达。结果:与对照组相比,模型组SOD活性显著性下降,MDA含量显著升高(P<0.05);显微镜下,心肌组织间隙变大,炎性细胞浸润,出血坏死细胞增加,TGF-β1表达明显增加(P<0.05);与模型组比较,FE组小鼠心重指数明显下降,SOD活性显著提高,MDA含量明显下降(P<0.05),心肌组织病理学改变缓解,TGF-β1表达明显下调(P<0.05),且呈一定剂量依赖性。结论:FE治疗对抗Iso诱导心肌纤维化,其机制可能与清除氧自由基,减轻脂质过氧化损伤及抑制TGF-β1表达有关。
引文
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