隔药饼灸对动脉粥样硬化兔血管内皮修复与基质细胞衍生因子1的影响
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摘要
目的:观察隔药饼灸对动脉粥样硬化兔受损血管内皮结构的修复以及基质细胞衍生因子1(stromal cells derived factor 1,SDF-1)含量的影响。方法:将75只兔随机分为正常组、模型组、直接灸组、阿托伐他汀钙组与隔药饼灸组,每组15只,正常组采用普通饲料喂养,其余各组采用高胆固醇饲养法喂养12周制备动脉粥样硬化兔模型。隔药饼灸组与直接灸组选取两组穴位:"巨阙""天枢""丰隆"穴;"心俞""肝俞""脾俞"穴。每组穴隔日交替施灸,每穴灸30 min,每日1次,连续4周。阿托伐他汀钙组每天将阿托伐他汀钙片(1.96 mg·kg-1·d-1)碾成粉末后拌入第1餐饲料中喂食,造模后正常组、模型组不予上述干预处理,此3组同灸法时固定。以酶法测定总胆固醇(TC)、三酰甘油(TG)的含量;比色法测定低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)的含量;光学显微镜下观察兔主动脉血管壁形态结构;酶联免疫吸附法测定血清SDF-1的含量。结果:干预后,与正常组比较,模型组TC、TG、LDL-C明显升高(均P<0.01),HDL-C明显降低(P<0.01);与模型组比较,直接灸组、阿托伐他汀钙组、隔药饼灸组TC、TG、LDL-C均明显降低(均P<0.01),HDL-C升高(P<0.01,P<0.05)。与正常组相比,模型组主动脉壁结构受损明显;与模型组相比,阿托伐他汀钙组、隔药饼灸组内皮结构有显著好转,直接灸组的主动脉内皮病理变化也有减轻。干预后,与模型组相比,直接灸组、阿托伐他汀钙组、隔药饼灸组血清中SDF-1水平有不同程度的升高(P<0.05,P<0.01),隔药饼灸组血清中SDF-1水平高于直接灸组(P<0.05)。结论:隔药饼灸能促进血清SDF-1的表达,修复受损血管内皮结构。
Objective To observe the effects of herbal-cake-separated moxibustion on the repair of damaged vascular endothelium structure and the content of stromal cells derived factor 1(SDF-1) in rabbits with atherosclerosis.Methods A total of 75 rabbits were randomly divided into a normal group, a model group, a direct moxibustion group,an atorvastatin calcium group and a herbal-cake-separated moxibustion group, 15 rabbits in each one. The rabbits in the normal group were fed with normal diet, and the remaining rabbits were fed with high-cholesterol diet for 12 weeks to prepare atherosclerotic model. Two groups of acupoints, one was "Juque"(CV 14), "Tianshu"(ST 25) and "Fenglong"(ST 40), the other one was "Xinshu"(BL 15), "Ganshu"(BL 18) and "Pishu"(BL 20), were applied in the direct moxibustion group and herbal-cake-separated moxibustion group; the two groups of acupoints were selected alternatively every other day. The moxibustion was given for 30 min per treatment, once a day for 4 weeks. The rabbits in the atorvastatin calcium group were treated with atorvastatin calcium tablets(1.96 mg·kg-1·d-1) which were crushed into powder and mixed into breakfast. After modeling, the rabbits in the normal group and model group received no treatment,and immobilized at the time when moxibustion was applied in other three groups. The levels of total cholesterol(TC)and triglyceride(TG) were measured by enzymic method; the low-density lipoprotein cholesterol(LDL-C) and high-density lipoprotein cholesterol(HDL-C) were measured by colorimetric method; the morphological structure of aortic wall was observed under optical microscope; the serum level of SDF-1 was determined by enzyme-linked immunosorbent assay(ELISA). Results After treatment, compared with the normal group, the levels of TC, TG and LDL-C were significantly increased in the model group(all P<0.01), and the level of HDL-C was decreased(P<0.01). Compared with the model group, the levels of TC, TG and LDL-C were significantly decreased(all P<0.01), and the level of HDL-C was significantly increased in the direct moxibustion group, atorvastatin calcium group and herbal-cake-separated moxibustion group(P<0.01, P<0.05). Compared with the normal group, the morphological structure of aortic wall was significantly damaged in the model group. Compared with the model group, the vascular endothelial structure was improved in the atorvastatin calcium group and herbal-cake-separated moxibustion group, and the pathological change of aorta endothelial in the direct moxibustion group was relieved. After treatment, compared with the model group, the level of SDF-1 was increased in the direct moxibustion group, atorvastatin calcium group and herbal-cake-separated moxibustion group(P<0.05, P<0.01); the level of SDF-1 in the herbal-cakeseparated moxibustion group was higher than that in the direct moxibustion group(P<0.05). Conclusion The herbalcake-separated moxibustion can promote the expression of SDF-1 in serum and repair the damaged aortic endothelial structure.
Objective To observe the effects of herbal-cake-separated moxibustion on the repair of damaged vascular endothelium structure and the content of stromal cells derived factor 1(SDF-1) in rabbits with atherosclerosis.Methods A total of 75 rabbits were randomly divided into a normal group, a model group, a direct moxibustion group,an atorvastatin calcium group and a herbal-cake-separated moxibustion group, 15 rabbits in each one. The rabbits in the normal group were fed with normal diet, and the remaining rabbits were fed with high-cholesterol diet for 12 weeks to prepare atherosclerotic model. Two groups of acupoints, one was "Juque"(CV 14), "Tianshu"(ST 25) and "Fenglong"(ST 40), the other one was "Xinshu"(BL 15), "Ganshu"(BL 18) and "Pishu"(BL 20), were applied in the direct moxibustion group and herbal-cake-separated moxibustion group; the two groups of acupoints were selected alternatively every other day. The moxibustion was given for 30 min per treatment, once a day for 4 weeks. The rabbits in the atorvastatin calcium group were treated with atorvastatin calcium tablets(1.96 mg·kg-1·d-1) which were crushed into powder and mixed into breakfast. After modeling, the rabbits in the normal group and model group received no treatment,and immobilized at the time when moxibustion was applied in other three groups. The levels of total cholesterol(TC)and triglyceride(TG) were measured by enzymic method; the low-density lipoprotein cholesterol(LDL-C) and high-density lipoprotein cholesterol(HDL-C) were measured by colorimetric method; the morphological structure of aortic wall was observed under optical microscope; the serum level of SDF-1 was determined by enzyme-linked immunosorbent assay(ELISA). Results After treatment, compared with the normal group, the levels of TC, TG and LDL-C were significantly increased in the model group(all P<0.01), and the level of HDL-C was decreased(P<0.01). Compared with the model group, the levels of TC, TG and LDL-C were significantly decreased(all P<0.01), and the level of HDL-C was significantly increased in the direct moxibustion group, atorvastatin calcium group and herbal-cake-separated moxibustion group(P<0.01, P<0.05). Compared with the normal group, the morphological structure of aortic wall was significantly damaged in the model group. Compared with the model group, the vascular endothelial structure was improved in the atorvastatin calcium group and herbal-cake-separated moxibustion group, and the pathological change of aorta endothelial in the direct moxibustion group was relieved. After treatment, compared with the model group, the level of SDF-1 was increased in the direct moxibustion group, atorvastatin calcium group and herbal-cake-separated moxibustion group(P<0.05, P<0.01); the level of SDF-1 in the herbal-cakeseparated moxibustion group was higher than that in the direct moxibustion group(P<0.05). Conclusion The herbalcake-separated moxibustion can promote the expression of SDF-1 in serum and repair the damaged aortic endothelial structure.
引文
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[6]Yu P,Zhang Z,Li S,et al.Progesterone modulates endothelial progenitor cell(EPC)viability through the CXCL12/CXCR4/PI3K/Akt signalling pathway[J].Cell Proliferat,2016,49(1):48-57.
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[9]卢享君.隔药饼灸对动脉粥样硬化兔胆固醇逆转运ABCA1的调节作用[D].长沙:湖南中医药大学,2016.
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[11]刘耀萌,崔莹雪,哈略,等.艾灸及艾烟对动脉粥样硬化模型小鼠血清TNF-α、hs-CRP及vWF的影响[J].中华中医药杂志,2016,31(4):1377-1379.
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[14]常小荣,严洁,易受乡,等.隔药饼灸治疗血脂异常的临床研究[J].中华中医药学刊,2010,28(1):8-10.
[15]黄洁,曾文洁,常小荣.常小荣运用灸法治疗高脂血症经验[J].湖南中医杂志,2015,31(3):21-22.
[16]刘未艾,常小荣,刘密,等.不同灸量隔药饼灸对高脂血症患者血脂及血液流变学的影响[J].辽宁中医杂志,2013,40(9):1787-1790.
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[18]李忠仁.实验针灸学[M].北京:中国中医药出版社,2003:314-319.
[19]岳增辉,严洁,常小荣,等.隔药饼灸对高脂血症兔主动脉内皮细胞超微结构的影响[J].中国针灸,2005,25(1):64-67.
[20]岳增辉,常小荣,严洁,等.隔药饼灸对兔高脂血症合并动脉粥样硬化主动脉血管细胞粘附分子-1mRNA表达的影响[J].针刺研究,2006,31(3):145-148.
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