摘要
目的观察水芹总酚酸对Friend鼠白血病病毒(Fr. MuLV)感染小鼠模型的作用。方法 Fr. MuLV病毒感染BALB/c小鼠造模,水芹总酚酸125、250、500mg·kg-1灌胃给药,每天1次,连续28d。末次给药后眼球取血,取脏器。计算小鼠脾指数和胸腺指数,ELISA试剂盒检测外周血CD_4~+、CD_8~+分子含量,RT-PCR方法检测脾脏病毒载量。结果水芹总酚酸250、500mg·kg-1组CD_4~+分子含量及CD_4~+/CD_8~+值明显提高,小鼠脾内Fr. MuLV降低,抑制率分别为46.95%和71.80%(P <0.05)。结论水芹总酚酸可改善小鼠的免疫抑制,降低脾内病毒载量。
Objective To evaluate the effects of total phenolics acid extracted from Oenanthe Javanica(OJTPA)on Friend murine leukemia virus(Fr. MuLV)infected BALB/c mice model.Methods The model was established by infecting BALB/c mice with Fr. MuLV.OJTPA 125,250 and 500 mg·kg-1,respectively,were administered intragastriccally for 28 days.The spleen index and thymus index of these mice were calculated.The contents of CD_4~+and CD_8~+ molecules in peripheral blood were determined by ELISA method.RT-PCR method was applied to determine the viral load in the spleen.Results High and middle doses of OJTPA(500 and 250 mg·kg-1)increased significantly CD_4~+content and CD_4~+/CD_8~+ ratio,but decreased the Fr. MuLV load in the spleen.The virus inhibition rates of middle and high dosages of OJTPA reached 46.95%and 71.80%(P <0.05),respectively.Conclusion OJTPA can improve immune suppression in mice and decrease the load of Fr. MuLV in the spleen.
引文
[1]黄正明,杨新波,曹文斌.水芹的本草考证[J].中草药,2001,32(1):59-62
[2]王选举,黄正明,杨新波,等.水芹总酚酸对2.2.15细胞HBVDNA、cccDNA的抑制作用[J].中国药理学通报,2009,25(8):1099-1102
[3]王选举,黄正明,杨新波,等.水芹总酚酸对2.2.15细胞分泌HBsAg与HBeAg的抑制作用[J].解放军药学学报,2009,25(2):148-151
[4]王选举,黄正明,杨新波,等.水芹总酚酸对鸭乙肝病毒DNA的抑制作用[J].解放军药学学报,2009,25(6):501-505
[5]王晨吟,刘青川,黄正明.水芹总酚酸对人类免疫缺陷病毒逆转录酶的抑制作用[J].中国医药导报,2013,10(12):4-8
[6]姜晶,崔晓兰,时宇静,等.Fr.MuLV小鼠红白血病模型的建立及叠氮胸苷抗病毒作用的研究[J].中国药理学通报,2008,24(11):1489-1491
[7] Joh AK,Ruth MR.Animal model for anti-AIDS therapy[J].AntivirRes,1992,19(2):81-109
[8]曾春娇.鼠艾滋病模型血浆病毒载量检测方法的建立及用于中药效果评价[D].广州:广州中医药大学,2008
[9]张伟,黄正明,陈晓农.水芹总酚酸对正常小鼠免疫功能的影响[J].解放军药学学报,2013,29(1):17-19