水芹总酚酸对Fr.MuLV感染小鼠的抗病毒作用
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摘要
目的观察水芹总酚酸对Friend鼠白血病病毒(Fr. MuLV)感染小鼠模型的作用。方法 Fr. MuLV病毒感染BALB/c小鼠造模,水芹总酚酸125、250、500mg·kg-1灌胃给药,每天1次,连续28d。末次给药后眼球取血,取脏器。计算小鼠脾指数和胸腺指数,ELISA试剂盒检测外周血CD_4~+、CD_8~+分子含量,RT-PCR方法检测脾脏病毒载量。结果水芹总酚酸250、500mg·kg-1组CD_4~+分子含量及CD_4~+/CD_8~+值明显提高,小鼠脾内Fr. MuLV降低,抑制率分别为46.95%和71.80%(P <0.05)。结论水芹总酚酸可改善小鼠的免疫抑制,降低脾内病毒载量。
Objective To evaluate the effects of total phenolics acid extracted from Oenanthe Javanica(OJTPA)on Friend murine leukemia virus(Fr. MuLV)infected BALB/c mice model.Methods The model was established by infecting BALB/c mice with Fr. MuLV.OJTPA 125,250 and 500 mg·kg-1,respectively,were administered intragastriccally for 28 days.The spleen index and thymus index of these mice were calculated.The contents of CD_4~+and CD_8~+ molecules in peripheral blood were determined by ELISA method.RT-PCR method was applied to determine the viral load in the spleen.Results High and middle doses of OJTPA(500 and 250 mg·kg-1)increased significantly CD_4~+content and CD_4~+/CD_8~+ ratio,but decreased the Fr. MuLV load in the spleen.The virus inhibition rates of middle and high dosages of OJTPA reached 46.95%and 71.80%(P <0.05),respectively.Conclusion OJTPA can improve immune suppression in mice and decrease the load of Fr. MuLV in the spleen.
Objective To evaluate the effects of total phenolics acid extracted from Oenanthe Javanica(OJTPA)on Friend murine leukemia virus(Fr. MuLV)infected BALB/c mice model.Methods The model was established by infecting BALB/c mice with Fr. MuLV.OJTPA 125,250 and 500 mg·kg-1,respectively,were administered intragastriccally for 28 days.The spleen index and thymus index of these mice were calculated.The contents of CD_4~+and CD_8~+ molecules in peripheral blood were determined by ELISA method.RT-PCR method was applied to determine the viral load in the spleen.Results High and middle doses of OJTPA(500 and 250 mg·kg-1)increased significantly CD_4~+content and CD_4~+/CD_8~+ ratio,but decreased the Fr. MuLV load in the spleen.The virus inhibition rates of middle and high dosages of OJTPA reached 46.95%and 71.80%(P <0.05),respectively.Conclusion OJTPA can improve immune suppression in mice and decrease the load of Fr. MuLV in the spleen.
引文
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[3]王选举,黄正明,杨新波,等.水芹总酚酸对2.2.15细胞分泌HBsAg与HBeAg的抑制作用[J].解放军药学学报,2009,25(2):148-151
[4]王选举,黄正明,杨新波,等.水芹总酚酸对鸭乙肝病毒DNA的抑制作用[J].解放军药学学报,2009,25(6):501-505
[5]王晨吟,刘青川,黄正明.水芹总酚酸对人类免疫缺陷病毒逆转录酶的抑制作用[J].中国医药导报,2013,10(12):4-8
[6]姜晶,崔晓兰,时宇静,等.Fr.MuLV小鼠红白血病模型的建立及叠氮胸苷抗病毒作用的研究[J].中国药理学通报,2008,24(11):1489-1491
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[8]曾春娇.鼠艾滋病模型血浆病毒载量检测方法的建立及用于中药效果评价[D].广州:广州中医药大学,2008
[9]张伟,黄正明,陈晓农.水芹总酚酸对正常小鼠免疫功能的影响[J].解放军药学学报,2013,29(1):17-19