西瓜枯萎病苗期抗性鉴定及抗源筛选
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摘要
为建立快速、有效的西瓜枯萎病苗期抗性鉴定技术体系,筛选抗枯萎病种质资源。对西瓜枯萎病苗期鉴定的接种方法、接种浓度、栽培土质、培养容器等进行系统筛选与比较,并应用筛选出的最佳技术对40份西瓜种质资源进行抗枯萎病鉴定与评价。结果表明,伤根蘸孢法可导致西瓜幼苗发病均匀、迅速,是较为理想的接种方法,最适分生孢子接种浓度为105个/m L,接种后用草炭土作为栽培土质,在培养瓶中保湿培养为最佳。40份西瓜种质材料鉴定结果显示,伤根蘸孢法是抗枯萎病快速鉴定的有效方法。从40份种质资源中筛选出2份对西瓜枯萎病有一定耐病性的抗性材料。
引文
[1]郑毕,毕扬,云晓敏,等.西瓜枯萎病的研究进展及其防治[J].中国植保导刊,2007,27(2):11-13.
[2]丁建成,张其安,方凌.西瓜新品种抗枯萎病鉴定[J].中国瓜菜,2005,18(6):27-29.
[3]Zhang Z G,Zhang J Y,Wang Y C,et al.Molecular detection of Fusarium oxysporum f.sp.niveum and Mycosphaerella melonis in infected plant tissues and soil[J].FEMS Microbiology Letters,2005,249(1):39-47.
[4]Shi J,Mueller W,Beckman C H.Ultrastructural responses of vessel contact cells in cotton plants resistant or susceptible to infection by Fusarium oxysporum f.sp.vasinfectum[J].Physiological and Molecular Plant Pathology,1991,38(3):211-222.
[5]Diener A C,Ausubel F M.Resistance to Fusarium oxysporum 1,a dominant Arabidopsis disease-resistance gene,is not race specific[J].Genetics,2005,171(1):305-321.
[6]耿丽华,郭绍贵,吕桂云,等.西瓜枯萎病菌生理小种鉴定技术体系的建立和验证[J].中国蔬菜,2010(20):52-56.
[7]Zhou X G,Everts K L,Bruton B D.Race 3,a new and highly virulent race of Fusarium oxysporum f.sp niveum causing Fusarium wilt in watermelon[J].Plant Disease,2010,94(1):92-98.
[8]段会军,张彩英,李喜焕,等.河北省西瓜枯萎病菌生理小种鉴定与AFLP分析[J].中国农业科学,2007,40(5):925-931.
[9]段会军,张彩英,郭小敏,等.西瓜品种对枯萎病抗性鉴定研究[J].园艺学报,2004,34(6):757.
[10]王喜庆,贾云鹤,尤海波.黑龙江省西瓜枯萎病菌生理小种鉴定及抗病鉴定方法筛选[J].北方园艺,2013(2):103-107.
[11]Zhang X W,Huang X S,Gu Q S.A preliminary report on screening the resistance of watermelon varieties to Fusarium wilt[J].Acta Hort,1995,18(402):45-47.
[12]杨宇红,吕红豪,杨翠荣,等.甘蓝枯萎病苗期抗性鉴定技术及抗源筛选[J].植物保护学报,2011,38(5):425-431.
[13]吴学宏,郝京京,王红梅,等.西瓜枯萎病菌几种接种方法比较试验[J].长江蔬菜,2003(3):38-39.
[14]Zhou X G,Everts K L.Races and inoculum density of Fusarium oxysporum f.sp niveum in commercial watermelon fields in Maryland and Delaware[J].Plant Disease,2003,87(6):692-698.
[15]Njoroge S M,Riley M B,Keinath A P.Effect of incorporation of Brassica spp.residues on population densities of soilborne microorganisms and on damping-off and Fusarium wilt of watermelon[J].Plant Disease,2008,92(2):287-294.
[16]刘朋义,别之龙,彭斌,等.甜瓜品种抗枯萎病的苗期鉴定[J].中国瓜菜,2011,24(2):11-13.
[17]Wheeler T A,Rowe R.Influence of soil characteristic and assay techniques on quantification of Verticillium dahliae in Ohio soils[J].Plant Disease,1995,79(1):29-34.
[18]李伦光.土壤因子对黄栌枯萎病发生的影响及病害防治的初步研究[D].北京:北京林业大学,2008.
[19]刘朋义.甜瓜枯萎病苗期接种鉴定体系建立及其应用[D].武汉:华中农业大学,2011.
[20]寇清荷,梁志怀,王志伟,等.西瓜枯萎病菌生理小种鉴定与抗病育种研究进展[J].中国蔬菜,2012(14):9-17.
[2]丁建成,张其安,方凌.西瓜新品种抗枯萎病鉴定[J].中国瓜菜,2005,18(6):27-29.
[3]Zhang Z G,Zhang J Y,Wang Y C,et al.Molecular detection of Fusarium oxysporum f.sp.niveum and Mycosphaerella melonis in infected plant tissues and soil[J].FEMS Microbiology Letters,2005,249(1):39-47.
[4]Shi J,Mueller W,Beckman C H.Ultrastructural responses of vessel contact cells in cotton plants resistant or susceptible to infection by Fusarium oxysporum f.sp.vasinfectum[J].Physiological and Molecular Plant Pathology,1991,38(3):211-222.
[5]Diener A C,Ausubel F M.Resistance to Fusarium oxysporum 1,a dominant Arabidopsis disease-resistance gene,is not race specific[J].Genetics,2005,171(1):305-321.
[6]耿丽华,郭绍贵,吕桂云,等.西瓜枯萎病菌生理小种鉴定技术体系的建立和验证[J].中国蔬菜,2010(20):52-56.
[7]Zhou X G,Everts K L,Bruton B D.Race 3,a new and highly virulent race of Fusarium oxysporum f.sp niveum causing Fusarium wilt in watermelon[J].Plant Disease,2010,94(1):92-98.
[8]段会军,张彩英,李喜焕,等.河北省西瓜枯萎病菌生理小种鉴定与AFLP分析[J].中国农业科学,2007,40(5):925-931.
[9]段会军,张彩英,郭小敏,等.西瓜品种对枯萎病抗性鉴定研究[J].园艺学报,2004,34(6):757.
[10]王喜庆,贾云鹤,尤海波.黑龙江省西瓜枯萎病菌生理小种鉴定及抗病鉴定方法筛选[J].北方园艺,2013(2):103-107.
[11]Zhang X W,Huang X S,Gu Q S.A preliminary report on screening the resistance of watermelon varieties to Fusarium wilt[J].Acta Hort,1995,18(402):45-47.
[12]杨宇红,吕红豪,杨翠荣,等.甘蓝枯萎病苗期抗性鉴定技术及抗源筛选[J].植物保护学报,2011,38(5):425-431.
[13]吴学宏,郝京京,王红梅,等.西瓜枯萎病菌几种接种方法比较试验[J].长江蔬菜,2003(3):38-39.
[14]Zhou X G,Everts K L.Races and inoculum density of Fusarium oxysporum f.sp niveum in commercial watermelon fields in Maryland and Delaware[J].Plant Disease,2003,87(6):692-698.
[15]Njoroge S M,Riley M B,Keinath A P.Effect of incorporation of Brassica spp.residues on population densities of soilborne microorganisms and on damping-off and Fusarium wilt of watermelon[J].Plant Disease,2008,92(2):287-294.
[16]刘朋义,别之龙,彭斌,等.甜瓜品种抗枯萎病的苗期鉴定[J].中国瓜菜,2011,24(2):11-13.
[17]Wheeler T A,Rowe R.Influence of soil characteristic and assay techniques on quantification of Verticillium dahliae in Ohio soils[J].Plant Disease,1995,79(1):29-34.
[18]李伦光.土壤因子对黄栌枯萎病发生的影响及病害防治的初步研究[D].北京:北京林业大学,2008.
[19]刘朋义.甜瓜枯萎病苗期接种鉴定体系建立及其应用[D].武汉:华中农业大学,2011.
[20]寇清荷,梁志怀,王志伟,等.西瓜枯萎病菌生理小种鉴定与抗病育种研究进展[J].中国蔬菜,2012(14):9-17.