间歇性低压低氧预处理对SD大鼠放射性肝损伤的保护作用
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摘要
目的探讨间歇性低压低氧预处理对SD大鼠放射性肝损伤的保护作用。方法将24只雄性SD大鼠随机分为对照组(C组)、4 000m低压低氧预处理组(IHHP组)、单纯照射组(R组)和低压低氧预处理联合照射组(IHHP+R组),每组6只,对照组大鼠在常规环境下饲养,IHHP组大鼠通过低压低氧舱模拟海拔高度4 000m的高原环境,6h/次/d,5次/w,照射组大鼠照射总剂量DT=60Gy,2Gy/次/d,5次/w,照射完成后6h采下腔静脉血检测谷丙转氨酶(ALT)、谷草转氨酶(AST),肉眼观察大鼠肝脏的变化,HE染色显微镜下观察肝组织的结构变化。结果 IHHP+R组与R组比较,2组间ALT、AST值降低,差异有统计学意义(P<0.05)。肉眼可见IHHP+R组大鼠肝脏轻度充血,HE染色IHHP+R组肝脏中央静脉、肝窦扩张充血,肝细胞空泡样变性数量及程度较R组明显减轻。结论间歇性低压低氧预处理后对放射造成的SD大鼠的肝损伤有保护作用。
Objective To investigate the protective effect of intermittent hypobaric hypoxia preconditioning on radiation induced liver injury in SD rats.Methods 24 male SD rats were randomly divided into control group(C),hypobaric hypoxia preconditioning group(IHHP),irradiation group(R)and hypobaric hypoxia preconditioning combined with irradiation group(IHHP+R),and 6 rats in each group.The rats in C group were housed under normal conditions.The rats in IHHP group were challenged by low pressure hypoxia chamber simulated altitude of 4 000 maltitude,6 hours/time/day,5 times/week.The irradiation group rats irradiated with a total dose of DT=60 Gy,2 Gy/f/d,5 f/w.Six hours after the irradiation,venous blood was collected for detection of alanine aminotransferase(ALT)and aspartate aminotransferase(AST),and the changes of the liver of the rats were observed.The changes of liver tissues were observed by HE staining.Results IHHP+R group compared with the R group,the ALT and AST values of the two groups decreased,and the difference was statistically significant(P <0.05).In the IHHP+R group,the liver was slightly congested,and HE staining showed that the number and degree of hepatocyte vacuolar degeneration in group IHHP+R were significantly lower than those in R group.Conclusion Intermittent hypobaric hypoxia preconditioning has protective effects on radiation induced liver injury in SD rats.
Objective To investigate the protective effect of intermittent hypobaric hypoxia preconditioning on radiation induced liver injury in SD rats.Methods 24 male SD rats were randomly divided into control group(C),hypobaric hypoxia preconditioning group(IHHP),irradiation group(R)and hypobaric hypoxia preconditioning combined with irradiation group(IHHP+R),and 6 rats in each group.The rats in C group were housed under normal conditions.The rats in IHHP group were challenged by low pressure hypoxia chamber simulated altitude of 4 000 maltitude,6 hours/time/day,5 times/week.The irradiation group rats irradiated with a total dose of DT=60 Gy,2 Gy/f/d,5 f/w.Six hours after the irradiation,venous blood was collected for detection of alanine aminotransferase(ALT)and aspartate aminotransferase(AST),and the changes of the liver of the rats were observed.The changes of liver tissues were observed by HE staining.Results IHHP+R group compared with the R group,the ALT and AST values of the two groups decreased,and the difference was statistically significant(P <0.05).In the IHHP+R group,the liver was slightly congested,and HE staining showed that the number and degree of hepatocyte vacuolar degeneration in group IHHP+R were significantly lower than those in R group.Conclusion Intermittent hypobaric hypoxia preconditioning has protective effects on radiation induced liver injury in SD rats.
引文
[1]董天明,安宁豫.放射性肝损伤影像学研究现状[J].中国医学科学院学报,2013,35(6):694-697.
[2]赵增虎,刘俊堂,范青建.放射性肝损伤研究及防治进展[J].现代肿瘤医学,2011,19(10):2110-2113.
[3]KIM J,WANG S,HYUN J,et al.Hedgehog signaling is associated with liver response to fractionated irradiation in Mice[J].Cell Physiol Biochem,2016,40(1/2):263-276.
[4]DOI H,SHIOMI H,MASAI H,et al.Threshould doses and prediction of visually apparent liver dysfunction after stereotactic body radiation therapy in cirrhotic and normal livers using magnetic resonance imaging[J].J Radiat Res,2016,57(3):294-300.
[5]ZHANG Y,ZHOU Z N.Beneficial effects of intermittent hypobaric hypoxia on the body[J].Chin J Appl Physiol,2012,28(6):504-509.
[6]CHEN W,ZHENG R,BAADE P D,et al.Cancer statistics in China,2015[J].CA Cancer J Clin,2016,66(2):115-132.
[7]江林官,孟鸿宇,张火俊.放射性肝损伤的研究进展[J].世界华人消化杂志,2017,25(20):1811-1818.
[8]CHENG W,XIAO L,AINIWAER M,et al.Molecular responses of radiation-induced liver damage in rats[J].Mol Med Rep,2015,11(4):2592-2600.
[9]吴戈,肖蕾,张瑞丽,等.急性放射性肝损伤大鼠模型的建立与评价[J].中国实验动物学报,2015,23(2):178-181.
[10]BERTHRONG M,FAJARDO L F.Radiation injury in surgical pathology.part II.alimentary tract.[J].Am J Surg Pathol,1981,5(2):153-78.
[11]YUAN F,TENG X,GUO Z,et al.Chronic intermittent hypobaric hypoxia ameliorates endoplasmic reticulum stress mediated liver damage induced by fructose in rats[J].Life Sci,2015(121):40-45.
[12]WANG J,WU Y,YUAN F,et al.Chronic intermittent hypobaric hypoxia attenuates radiation induced heart damage in rats[J].Life Sci,2016(160):57-63.
[13]BRENNAN S,JACKON R,PATEL M,et al.Early opening of sarcolemmal ATP-sensitive potassium channels is not a key step in PKC-mediated[J].J Mol Cell Cardiol,2015(79):42-53.
[14]ZHU X H,YAN H C,ZHANG J,et al.Interrmittent hypoxia promotes hippocompal neurogenesis and produces antidepressant-like effects in adult rats[J].J Neurosci,2010,30(38):12653-12663.
[15]WANG C T,LIN H J,CHENG B C,et al.Attenuating systemic inflammatory markers in simulated high-altitude exposure by heat shock protein 70-mediated hypobaric hypoxia preconditioning in rats[J].J Formos Med Assoc,2015,114(4):328-338.
[16]YAN N,FENG Z G,YAN G T,et al.Effect of repeated hypoxia preconditioning on renal ischemia-reperfusioninduced hepatic dysfunction in rats[J].J South Med Univ,2015,35(1):149-153.
[17]MA H J,LI Q,MA H J,et al.Chronic intermittent hypobaric hypoxia ameliorates ischemia/reperfusion-induced calcium overload in heart via Na/Ca2+exchanger in developing rats[J].Cell Physiol Biochem,2014,34(2):313-324.
[18]KANEKAR S,BODGANOVA O V,OLSON P R,et al.Hypobaric hypoxia induces depression-like behavior in female sprague-dawley rats,but not in males[J].High Alt Med Biol,2015,16(1):52-60.
[19]KUSHWAH N,JAIN V,DEEP S,et al.Neuroprotective role of intermittent hypobaric hypoxia in unpredictable chronic mild stress induced derression in rats[J].PLoS One,2016,11(2):e0149309.
[20]XU K,LAMANNA J C.Short-term hypoxic preconditioning improved survival following cardiac arrest and resuscitation in rats[J].Adv Exp Med Biol,2014(812):309-315.
[21]PRPROKOV V V,GOLDOBENKO G V,NIKOLAEV A V,et al.Long-term results of surgery and preoperative hypoxyradiotherapy for colorectal cancer[J].Vopr Onkol,2011(57):99-101.
[22]孙衍伟,安永恒.低氧放疗对中晚期胰腺癌的放射防护作用[J].中华胰腺病杂志,2013,13(1):40-41.
[2]赵增虎,刘俊堂,范青建.放射性肝损伤研究及防治进展[J].现代肿瘤医学,2011,19(10):2110-2113.
[3]KIM J,WANG S,HYUN J,et al.Hedgehog signaling is associated with liver response to fractionated irradiation in Mice[J].Cell Physiol Biochem,2016,40(1/2):263-276.
[4]DOI H,SHIOMI H,MASAI H,et al.Threshould doses and prediction of visually apparent liver dysfunction after stereotactic body radiation therapy in cirrhotic and normal livers using magnetic resonance imaging[J].J Radiat Res,2016,57(3):294-300.
[5]ZHANG Y,ZHOU Z N.Beneficial effects of intermittent hypobaric hypoxia on the body[J].Chin J Appl Physiol,2012,28(6):504-509.
[6]CHEN W,ZHENG R,BAADE P D,et al.Cancer statistics in China,2015[J].CA Cancer J Clin,2016,66(2):115-132.
[7]江林官,孟鸿宇,张火俊.放射性肝损伤的研究进展[J].世界华人消化杂志,2017,25(20):1811-1818.
[8]CHENG W,XIAO L,AINIWAER M,et al.Molecular responses of radiation-induced liver damage in rats[J].Mol Med Rep,2015,11(4):2592-2600.
[9]吴戈,肖蕾,张瑞丽,等.急性放射性肝损伤大鼠模型的建立与评价[J].中国实验动物学报,2015,23(2):178-181.
[10]BERTHRONG M,FAJARDO L F.Radiation injury in surgical pathology.part II.alimentary tract.[J].Am J Surg Pathol,1981,5(2):153-78.
[11]YUAN F,TENG X,GUO Z,et al.Chronic intermittent hypobaric hypoxia ameliorates endoplasmic reticulum stress mediated liver damage induced by fructose in rats[J].Life Sci,2015(121):40-45.
[12]WANG J,WU Y,YUAN F,et al.Chronic intermittent hypobaric hypoxia attenuates radiation induced heart damage in rats[J].Life Sci,2016(160):57-63.
[13]BRENNAN S,JACKON R,PATEL M,et al.Early opening of sarcolemmal ATP-sensitive potassium channels is not a key step in PKC-mediated[J].J Mol Cell Cardiol,2015(79):42-53.
[14]ZHU X H,YAN H C,ZHANG J,et al.Interrmittent hypoxia promotes hippocompal neurogenesis and produces antidepressant-like effects in adult rats[J].J Neurosci,2010,30(38):12653-12663.
[15]WANG C T,LIN H J,CHENG B C,et al.Attenuating systemic inflammatory markers in simulated high-altitude exposure by heat shock protein 70-mediated hypobaric hypoxia preconditioning in rats[J].J Formos Med Assoc,2015,114(4):328-338.
[16]YAN N,FENG Z G,YAN G T,et al.Effect of repeated hypoxia preconditioning on renal ischemia-reperfusioninduced hepatic dysfunction in rats[J].J South Med Univ,2015,35(1):149-153.
[17]MA H J,LI Q,MA H J,et al.Chronic intermittent hypobaric hypoxia ameliorates ischemia/reperfusion-induced calcium overload in heart via Na/Ca2+exchanger in developing rats[J].Cell Physiol Biochem,2014,34(2):313-324.
[18]KANEKAR S,BODGANOVA O V,OLSON P R,et al.Hypobaric hypoxia induces depression-like behavior in female sprague-dawley rats,but not in males[J].High Alt Med Biol,2015,16(1):52-60.
[19]KUSHWAH N,JAIN V,DEEP S,et al.Neuroprotective role of intermittent hypobaric hypoxia in unpredictable chronic mild stress induced derression in rats[J].PLoS One,2016,11(2):e0149309.
[20]XU K,LAMANNA J C.Short-term hypoxic preconditioning improved survival following cardiac arrest and resuscitation in rats[J].Adv Exp Med Biol,2014(812):309-315.
[21]PRPROKOV V V,GOLDOBENKO G V,NIKOLAEV A V,et al.Long-term results of surgery and preoperative hypoxyradiotherapy for colorectal cancer[J].Vopr Onkol,2011(57):99-101.
[22]孙衍伟,安永恒.低氧放疗对中晚期胰腺癌的放射防护作用[J].中华胰腺病杂志,2013,13(1):40-41.