疏肝化瘀方对冠心病心肌缺血大鼠HGF蛋白表达及心功能的影响
|
摘要
目的:探讨疏肝化瘀方对冠心病心肌缺血大鼠HGF蛋白表达及心功能的影响。方法:选取健康雄性SD大鼠72只,采用随机数字表分为假手术组、模型组、阳性对照组、实验组各18只,假手术组和模型组仅给予等量生理盐水处理、阳性对照组给予麝香保心丸治疗、实验组给予疏肝化瘀方治疗;采用免疫组化技术检测各组大鼠干预14 d后的心肌组织中HGF蛋白表达,测定各组SD大鼠心脏功能参数及血清乳酸脱氢酶(LDH)、肌酸激酶(CK)、超氧化物歧化酶(SOD)、一氧化氮(NO)。结果:模型组大鼠心肌组织的HGF蛋白光密度值显著的高于假手术组(P<0.05);阳性对照组和实验组大鼠心肌组织的HGF蛋白光密度值显著的高于模型组(P<0.05);模型组大鼠LVEDP显著的高于假手术组(P<0.05),LVSP、+dp/dtmax、-dp/dtmax绝对值显著的低于假手术组(P<0.05);阳性对照组和实验组大鼠LVEDP显著的低于模型组(P<0.05),LVSP、+dp/dtmax、-dp/dtmax绝对值显著的高于模型组(P<0.05);模型组大鼠血清LDH、CK水平显著的高于假手术组(P<0.05),SOD、NO水平显著的低于假手术组(P<0.05);阳性对照组和实验组大鼠血清LDH、CK水平显著的低于模型组(P<0.05),SOD、NO水平显著的高于模型组(P<0.05)。结论:疏肝化瘀方治疗冠心病心肌缺血大鼠能显著改善其心功能、对心肌具有一定的保护作用,可提高心肌组织HGF蛋白表达水平。
Objective:To explore the effect of Shugan Huayu Recipe the treatment of myocardial ischemia on the expression of hepatocyte growth factor(HGF) protein and cardiac function from the treatment of myocardial ischemia.Methods:Selected 72 healthy male SD rats, were randomly divided into sham operation group, model group, positive control group, 18 rats in each experimental group, sham operation group and model group were given normal saline, the positive control group was given Shexiangbaoxin pill treatment, experimental group received Shugan Huayu Recipe treatment; the expression of HGF protein were detected by immunohistochemical methods in rats after 14 days of intervention in the myocardial tissue, determination of cardiac function parameters and serum LDH group of SD rats(LDH), creatine kinase(CK), superoxide dismutase(SOD), nitric oxide(NO).Results:The optical density of HGF protein in myocardium of model group was significantly higher than that of sham operation group(P<0.05). The optical density of HGF protein in myocardial tissue of model group was significantly higher than that of model group(P<0.05). LVEDP in model group were significantly higher than those in sham-operated group(P<0.05), LVSP and the absolute volue of + dp/dtmax,-dp/dtmax were significantly lower than those in sham-operated group. The LVEDP in the experimental group were significantly lower than those in the untreated group(P<0.05), but LVSP and + dp/dtmax, the absolute value of-dp/dtmax were significantly higher than those in the untreated group(P<0.05), and the levels of SOD and NO were significantly lower than those in the sham operation group(P<0.05). The levels of serum LDH and CK in the positive control group and the experimental group were significantly lower than those in the model group(P<0.05). The levels of SOD and NO were significantly higher than those in the model group(P<0.05).Conclusion:Shugan Huayu recipe can significantly improve its cardiac function, have some protective effect on the myocardium and increase the expression of HGF protein in myocardial tissue.
Objective:To explore the effect of Shugan Huayu Recipe the treatment of myocardial ischemia on the expression of hepatocyte growth factor(HGF) protein and cardiac function from the treatment of myocardial ischemia.Methods:Selected 72 healthy male SD rats, were randomly divided into sham operation group, model group, positive control group, 18 rats in each experimental group, sham operation group and model group were given normal saline, the positive control group was given Shexiangbaoxin pill treatment, experimental group received Shugan Huayu Recipe treatment; the expression of HGF protein were detected by immunohistochemical methods in rats after 14 days of intervention in the myocardial tissue, determination of cardiac function parameters and serum LDH group of SD rats(LDH), creatine kinase(CK), superoxide dismutase(SOD), nitric oxide(NO).Results:The optical density of HGF protein in myocardium of model group was significantly higher than that of sham operation group(P<0.05). The optical density of HGF protein in myocardial tissue of model group was significantly higher than that of model group(P<0.05). LVEDP in model group were significantly higher than those in sham-operated group(P<0.05), LVSP and the absolute volue of + dp/dtmax,-dp/dtmax were significantly lower than those in sham-operated group. The LVEDP in the experimental group were significantly lower than those in the untreated group(P<0.05), but LVSP and + dp/dtmax, the absolute value of-dp/dtmax were significantly higher than those in the untreated group(P<0.05), and the levels of SOD and NO were significantly lower than those in the sham operation group(P<0.05). The levels of serum LDH and CK in the positive control group and the experimental group were significantly lower than those in the model group(P<0.05). The levels of SOD and NO were significantly higher than those in the model group(P<0.05).Conclusion:Shugan Huayu recipe can significantly improve its cardiac function, have some protective effect on the myocardium and increase the expression of HGF protein in myocardial tissue.
引文
[1] 刘征, 冯其茂, 董耀荣,等. 通心方对慢性心肌缺血小猪冠脉侧支循环的研究[J]. 中华中医药学刊, 2017,35(9):2356-2361.
[2] 刘兆玉, 张迪泽, 林江,等. 纤维介素2对糖尿病大鼠心功能及肝细胞生长因子/c-Met信号通路影响的观察[J]. 中国糖尿病杂志, 2017, 25(8):737-742.
[3] 于俊民, 张小波, 李大鹤,等. 厄贝沙坦对大鼠梗死心肌组织中HGFmRNA和蛋白水平的影响[J]. 中国病理生理杂志, 2017, 33(6):1134-1137.
[4] 王芊芊, 刘梦琳, 彭靖,等. 从肝治心组方对大鼠缺血心肌血管新生及HGF蛋白表达的影响[J]. 中医药导报, 2016,18(8):16-19.
[5] 魏晓露, 李春雨, 苏玮莲,等. 扶肾降浊方对肾间质纤维化大鼠的治疗作用及TGF-β_1,HGF,ColⅠ蛋白表达的影响[J]. 中国实验方剂学杂志, 2016,28(6):114-118.
[6] 马海浓,方来福,刘敏杰. P27和Stathmin在宫颈癌组织中的表达及其相互关系[J].中国现代医生,2016 ,54(29):4-8.
[7] 王雪臣, 刘震宇, 范立华,等. 李庆海从肝论治难治性高血压经验[J]. 中医学报, 2017, 32(6):956-959.
[8] 耿学斌, 李莉, 王宏岭,等. 肝细胞生长因子治疗大鼠冠状动脉粥样硬化的作用及机制[J]. 中华实验外科杂志, 2015, 32(7):1613-1615.
[9] Henry TD, Hirsch AT, Goldman J, et al. Safety of a non-viral plasmid-encoding dual isoforms of hepatocyte growth factor in critical limb ischemia patients[J]. Gene Therapy, 2015, 18(8):788-794.
[10] Zhao L, Liu X, Zhang Y, et al. Enhanced cell survival and paracrine effects of mesenchymal stem cells overexpressing hepatocyte growth factor promote cardioprotection in myocardial infarction[J]. Experimental Cell Research, 2016, 344(1):30.
[11] Pérez-Calvo JI, Morales-Rull JL, Gimeno-Orna JA, et al. Usefulness of the Hepatocyte Growth Factor as a Predictor of Mortality in Patients Hospitalized With Acute Heart Failure Regardless of Ejection Fraction[J]. American Journal of Cardiology, 2016, 118(4):543-549.
[12] 高丹丹, 付佳, 秦波,等. HIL-6和HGF双基因重组腺病毒增强对慢加急性肝衰竭大鼠的治疗效果[J]. 中国病理生理杂志, 2016, 32(4):707-712.
[13] Lee SC, Jeong HJ, Choi BJ, et al. Role of the spleen in liver regeneration in relation to transforming growth factor and hepatocyte growth factor[J]. Journal of Surgical Research, 2015, 196(2):270-277.
[14] 王金, 赵一洁, 崔广宾,等. HGF蛋白与C-Met蛋白在胆管癌中的表达及临床意义[J]. 重庆医学, 2016, 45(17):2362-2364.
[15] 杨璋斌, 李安, 杨璇,等. 芎芪颗粒对肾间质纤维化大鼠肾组织HGF、MMP-3、TIMP-1的影响[J]. 中华中医药杂志, 2016,29(6):2142-2145.
[16] 于向荣. 复心汤对大鼠急性心肌缺血及左室舒张功能的保护作用[J]. 宜春学院学报,2007,29(4):118-119.
[17] 王俊东,崔勇,王建国,等. 人参皂苷Rh3预处理对大鼠心肌缺血再灌注损伤的保护作用研究[J]. 中华中医药学刊,2017,35(11):2783-2786.
[18] 徐萍,石月萍. 加减枳实薤白桂枝汤对大鼠心肌缺血再灌注过氧化损伤的影响[J]. 中药药理与临床,2017,33(3):14-18.
[2] 刘兆玉, 张迪泽, 林江,等. 纤维介素2对糖尿病大鼠心功能及肝细胞生长因子/c-Met信号通路影响的观察[J]. 中国糖尿病杂志, 2017, 25(8):737-742.
[3] 于俊民, 张小波, 李大鹤,等. 厄贝沙坦对大鼠梗死心肌组织中HGFmRNA和蛋白水平的影响[J]. 中国病理生理杂志, 2017, 33(6):1134-1137.
[4] 王芊芊, 刘梦琳, 彭靖,等. 从肝治心组方对大鼠缺血心肌血管新生及HGF蛋白表达的影响[J]. 中医药导报, 2016,18(8):16-19.
[5] 魏晓露, 李春雨, 苏玮莲,等. 扶肾降浊方对肾间质纤维化大鼠的治疗作用及TGF-β_1,HGF,ColⅠ蛋白表达的影响[J]. 中国实验方剂学杂志, 2016,28(6):114-118.
[6] 马海浓,方来福,刘敏杰. P27和Stathmin在宫颈癌组织中的表达及其相互关系[J].中国现代医生,2016 ,54(29):4-8.
[7] 王雪臣, 刘震宇, 范立华,等. 李庆海从肝论治难治性高血压经验[J]. 中医学报, 2017, 32(6):956-959.
[8] 耿学斌, 李莉, 王宏岭,等. 肝细胞生长因子治疗大鼠冠状动脉粥样硬化的作用及机制[J]. 中华实验外科杂志, 2015, 32(7):1613-1615.
[9] Henry TD, Hirsch AT, Goldman J, et al. Safety of a non-viral plasmid-encoding dual isoforms of hepatocyte growth factor in critical limb ischemia patients[J]. Gene Therapy, 2015, 18(8):788-794.
[10] Zhao L, Liu X, Zhang Y, et al. Enhanced cell survival and paracrine effects of mesenchymal stem cells overexpressing hepatocyte growth factor promote cardioprotection in myocardial infarction[J]. Experimental Cell Research, 2016, 344(1):30.
[11] Pérez-Calvo JI, Morales-Rull JL, Gimeno-Orna JA, et al. Usefulness of the Hepatocyte Growth Factor as a Predictor of Mortality in Patients Hospitalized With Acute Heart Failure Regardless of Ejection Fraction[J]. American Journal of Cardiology, 2016, 118(4):543-549.
[12] 高丹丹, 付佳, 秦波,等. HIL-6和HGF双基因重组腺病毒增强对慢加急性肝衰竭大鼠的治疗效果[J]. 中国病理生理杂志, 2016, 32(4):707-712.
[13] Lee SC, Jeong HJ, Choi BJ, et al. Role of the spleen in liver regeneration in relation to transforming growth factor and hepatocyte growth factor[J]. Journal of Surgical Research, 2015, 196(2):270-277.
[14] 王金, 赵一洁, 崔广宾,等. HGF蛋白与C-Met蛋白在胆管癌中的表达及临床意义[J]. 重庆医学, 2016, 45(17):2362-2364.
[15] 杨璋斌, 李安, 杨璇,等. 芎芪颗粒对肾间质纤维化大鼠肾组织HGF、MMP-3、TIMP-1的影响[J]. 中华中医药杂志, 2016,29(6):2142-2145.
[16] 于向荣. 复心汤对大鼠急性心肌缺血及左室舒张功能的保护作用[J]. 宜春学院学报,2007,29(4):118-119.
[17] 王俊东,崔勇,王建国,等. 人参皂苷Rh3预处理对大鼠心肌缺血再灌注损伤的保护作用研究[J]. 中华中医药学刊,2017,35(11):2783-2786.
[18] 徐萍,石月萍. 加减枳实薤白桂枝汤对大鼠心肌缺血再灌注过氧化损伤的影响[J]. 中药药理与临床,2017,33(3):14-18.