响应面优化汽化过氧化氢杀灭PET瓶内表面枯草芽孢杆菌工艺
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摘要
研究汽化过氧化氢对聚对苯二甲酸乙二醇酯(Polyethylene Terephthalate,PET)瓶坯内表面上枯草芽孢杆菌的杀菌效果。采用单因素试验结合Box-Behnken中心试验设计原理,对过氧化氢溶液流量、灭菌时间和灭菌温度3种灭菌工艺条件进行了响应面优化分析。汽化过氧化氢杀灭PET瓶内表面枯草芽孢杆菌的最佳工艺条件为:过氧化氢溶液流量14 mL/min,灭菌时间9s,灭菌温度120℃,在此条件下汽化过氧化氢对枯草芽孢杆菌的杀菌对数值可达5.40 log。为汽化过氧化氢灭菌的工业化应用提供理论依据。
This study aims to inactivate Bacillus subtilis in Polyethylene Terephthalate(PET)preforms by hydrogen peroxide vapour. Based on single factor experiment combined with Box-Behnken central combination design,sterilization processes,such as H_2O_2 flowrate,treatment and temperature,are optimized by response surface methodology. The optimum process parameters are:H_2O_2 flowrate= 14 mL/min,treatment time= 9 s,and temperature= 120 ℃.Under the optimized conditions,the reduction of Bacillus subtilis could be up to 5.40 log. It provides theoretical basis for the industrial application of hydrogen peroxide vapour sterilization.
This study aims to inactivate Bacillus subtilis in Polyethylene Terephthalate(PET)preforms by hydrogen peroxide vapour. Based on single factor experiment combined with Box-Behnken central combination design,sterilization processes,such as H_2O_2 flowrate,treatment and temperature,are optimized by response surface methodology. The optimum process parameters are:H_2O_2 flowrate= 14 mL/min,treatment time= 9 s,and temperature= 120 ℃.Under the optimized conditions,the reduction of Bacillus subtilis could be up to 5.40 log. It provides theoretical basis for the industrial application of hydrogen peroxide vapour sterilization.
引文
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[19]ZHANG Xiayun,ZHU Wenqing. Observation on germ icidal efficacy of vapor phase hydrogen peroxide the purification space[J].Chinese Journal of Disinfection,2011,28(3):260-262.(in Chinese)
[20]克罗内斯股份公司.用于包装容器杀菌的方法和装置.中国,CN008029555[P]. 2002-02-20.
[21]BJERBORN T. Method of sterilizing a package material. US 6682696[P]. 2004-01-27.
[22]吴有炜.试验设计与数据处理[M].苏州:苏州大学出版社,2002,115-154.
[23]LU Chen,ZOU Yuhong,ZHANG Shikang,et al. Optimization of ultrasound-assisted extraction of protein from tea residue by response surface methodology[J]. Journal of Food Science and Biotechnology,2012,31(3):319-325.(in Chinese)
[2]CARDOSO C,FARIA F,MIRANDA W,et al. Modeling of sporicidal effect of hydrogen peroxide in the sterilization of low density polyethylene film inoculated with Bacillus subtilis spores[J]. Food Control,2011,22(10):1559-1564.
[3]MENG J,GONG Y,QIAN P,et al. Combined effects of ultra-high hydrostatic pressure and mild heat on the inactivation of Bacillus subtilis[J]. Lwt-Food Science and Technology,2016,68:59-66.
[4]SIEMER C,TOEPFL S,HEINZ V. Inactivation of Bacillus subtilis spores by pulsed electric fields(PEF)in combination with thermal energy-I. Influence of process-and product parameters[J]. Food Control,2014,39:163-171.
[5]ZHANG M,OH J,HUANG S,et al. Priming with nano-aerosolized water and sequential dip-washing with hydrogen peroxide:An efficient sanitization method to inactivate Salmonella typhimurium LT2 on spinach[J]. Journal of Food Engineering,2015,161:8-15.
[6]SOMAVAT R,MOHAMED H,SASTRY S. Inactivation kinetics of Bacillus coagulans spores under ohmic and conventional heating[J]. Lwt-Food Science and Technology,2013,54(1):194-198.
[7]ZHANG Pingjun. Disinfection characteristic of the food gradehydrogen peroxideand its application in the food industry[J]. Dairy Industry China,2005,33(7):47-50.(in Chinese)
[8]BLUESKI Yu. Vaporized hydrogen peroxide sterilization and applications in animal facilities[J]. Pharmaceutical&Engineering Design,2012,33(2):49-53.(in Chinese)
[9]YU Xiaofeng. Advance in sterilization mechanism of hydrogen peroxide[J]. Chinese Journal of Disinfection,1988,5(3):149-152.(in Chinese)
[10]MALIK D,SHAW C,RIELLY C,et al. The inactivation of Bacillus subtilis spores at low concentrations of hydrogen peroxide vapour[J]. Journal of Food Engineering,2013,114(3):391-396.
[11]KIRCHNER P,LI B,SPELTHAHN H,et al. Thin-film calorimetric H2O2gas sensor for the validation of germicidal effectivity in aseptic filling processes[J]. Sensors and Actuators B-Chemical,2011,154(2):257-263.
[12]PRUSS K,STIRTZEL S,KULOZIK U. Influence of the surface temperature of packaging specimens on the inactivation of Bacillus spores by means of gaseous H2O2[J]. J Appl Microbiol,2012,112(3):493-501.
[13]SHI Yun. Discussion of application of VHP room temperature sterilization system[J]. Pharmaceutical&Engineering Design,2011,32(3):12-14.(in Chinese)
[14]DING Xiaoyue,LIANG Yi. The observation and exploration of the latest foreign low-temperature vaporized hydrogen peroxide(VHP)sterilization system[J]. Mechanical and Electrical Information,2010,26:45-47.(in Chinese)
[15]唐丽杰.微生物学实验[M].哈尔滨:哈尔滨工业大学出版社,2005:57-71.
[16]周德庆,徐德强.微生物学实验教程[M].北京:高等教育出版社,2013:99-102.
[17]UNGER-BIMCZOK B,KOTTKE V,HERTEL C,et al. The influence of humidity,hydrogen peroxide concentration,and condensation on the inactivation of Geobacillus stearothermophilus spores with hydrogen peroxide vapor[J]. Journal of Pharmaceutical Innovation,2008,3(2):123.
[18]WATLING D,PARKS M. The relationship between saturated hydrogen peroxide,water vapour and temperature[J].Pharmaceutical Technology Europe,2004,16(3):50.
[19]ZHANG Xiayun,ZHU Wenqing. Observation on germ icidal efficacy of vapor phase hydrogen peroxide the purification space[J].Chinese Journal of Disinfection,2011,28(3):260-262.(in Chinese)
[20]克罗内斯股份公司.用于包装容器杀菌的方法和装置.中国,CN008029555[P]. 2002-02-20.
[21]BJERBORN T. Method of sterilizing a package material. US 6682696[P]. 2004-01-27.
[22]吴有炜.试验设计与数据处理[M].苏州:苏州大学出版社,2002,115-154.
[23]LU Chen,ZOU Yuhong,ZHANG Shikang,et al. Optimization of ultrasound-assisted extraction of protein from tea residue by response surface methodology[J]. Journal of Food Science and Biotechnology,2012,31(3):319-325.(in Chinese)