寄主水果11种挥发性成分对斑翅果蝇的引诱效果
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摘要
测试了斑翅果蝇寄主水果11种挥发性成分(己醛、2-庚酮、反式-2-己烯醛、芳樟醇、乙酸乙酯、水杨酸甲酯、己酸、α-紫罗兰酮、己醇、反式-2-己烯-1-醇和乙酸异戊酯)分别与一定比例的乙醇和乙酸混合对斑翅果蝇成虫的引诱效果,以筛选出对乙醇和乙酸混合液具有增效作用的挥发物,并对具有增效作用的挥发物进行正交试验,以找出具有最佳引诱效果的配方.结果表明:将一定浓度的己醛、2-庚酮、反式-2-己烯醛和芳樟醇添加到乙醇和乙酸中对斑翅果蝇雌雄成虫的引诱具有显著的增效作用;正交试验结果表明,以各种挥发物作为溶质,乙醇和乙酸混合液作为溶剂,将体积分数为1/5 000的己醛、1/500的2-庚酮和1/5 000的芳樟醇混合到乙醇和乙酸中对斑翅果蝇的引诱效果最好,引诱率达到86.4%.
To find out compounds with synergism effect for ethanol and acetic acid mixtures for controlling Drosophila suzukii, the attractiveness of ethanol and acetic acid mixtures combined with 11 volatile compounds(hexanal, 2-heptanone, trans-2-hexenal, linalool, ethyl acetate, methyl salicylate, hexanoic acid, α-ionone, hexanol, trans-2-hexen-1-ol and isoamyl acetate) which were detected from host fruits to D.suzukii adults were determined, respectively. Moreover, the candidate compounds were evaluated by orthogonal experiment to develop an optimal prescription with best attractive efficacy. The results showed that ethanol and acetic acid mixtures combined with a certain concentration of hexanal, 2-heptanone, trans-2-hexenal and linalool had significant synergism effects on attracting D.suzukii adults. The orthogonal experiment, with the volatiles being solute and ethanol and acetic acid mixtures being solvent showed that mixture of 1/5 000 hexanal, 1/500 2-heptanone, and 1/5 000 linalool with ethanol and acetic acid showed the best attractiveness to D.suzukii adults with a capture rate of 86.4%.
To find out compounds with synergism effect for ethanol and acetic acid mixtures for controlling Drosophila suzukii, the attractiveness of ethanol and acetic acid mixtures combined with 11 volatile compounds(hexanal, 2-heptanone, trans-2-hexenal, linalool, ethyl acetate, methyl salicylate, hexanoic acid, α-ionone, hexanol, trans-2-hexen-1-ol and isoamyl acetate) which were detected from host fruits to D.suzukii adults were determined, respectively. Moreover, the candidate compounds were evaluated by orthogonal experiment to develop an optimal prescription with best attractive efficacy. The results showed that ethanol and acetic acid mixtures combined with a certain concentration of hexanal, 2-heptanone, trans-2-hexenal and linalool had significant synergism effects on attracting D.suzukii adults. The orthogonal experiment, with the volatiles being solute and ethanol and acetic acid mixtures being solvent showed that mixture of 1/5 000 hexanal, 1/500 2-heptanone, and 1/5 000 linalool with ethanol and acetic acid showed the best attractiveness to D.suzukii adults with a capture rate of 86.4%.
引文
[1] 张开春,闫国华,郭晓军,等.斑翅果蝇(Drosophila suzukii)研究现状[J].果树学报,2014,31(4):717-721.
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[6] 薛万琦,赵建铭.中国蝇类[M].沈阳:辽宁科学技术出版社,1996.
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[10] 李洋洋,王珏,原国辉,等.两种关键花香挥发物对棉铃虫的信息多义性[J].生态学杂志,2014,33(2):340-345.
[11] CHA D H,ADAMS T,ROGG H,et al.Identification and field evaluation of fermentation volatiles from wine and vinegar that mediate attraction of spotted wing drosophila,Drosophila suzukii[J].Journal of Chemical Ecology,2012,38(11):1 419-1 431.
[12] 伍苏然,太红坤,李正跃,等.樱桃果蝇田间诱捕方法比较[J].云南农业大学学报,2007,22(5):776-778.
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[14] HAMBY K A,BECHER P G.Current knowledge of interactions between Drosophila suzukii and microbes,and their potential utility for pest management[J].Journal of Pest Science,2016,89(3):621-630.
[15] 刘燕,谢冬生,胡纯华,等.β-石竹烯对斑翅果蝇雌成虫行为的影响[J].环境昆虫学报,2018,40(3):684-689.
[16] 刘燕,谢冬生,胡纯华,等.杨梅果实成熟度及挥发物对斑翅果蝇定向行为的影响[J].应用昆虫学报,2017,54(5):716-723.
[17] ABRAHAM J,ZHANG A,ANGELI S,et al.Behavioral and antennal responses of Drosophila suzukii (Diptera:Drosophilidae) to volatiles from fruit extracts[J].Environmental Entomology,2015,44(2):356-367.
[18] MAISTRI S.Drosophila suzukii (Matsumura) (Diptera:Drosophilidae):indagini biomolecolari e sensibilità agli attacchi di diverse cultivar di mirtillo (Vaccinium spp.)[D].Veneto,Italy:Università degli Studi di Padova,2012.
[19] HUANG J,GUT L,GRIESHOP M.Evaluation of food-based attractants for Drosophila suzukii (Diptera:Drosophilidae)[J].Environmental Entomology,2017,46(4):878-884.
[20] CAI P,YI C,ZHANG Q,et al.Evaluation of protein bait manufactured from brewery yeast waste for controlling Drosophila suzukii (Diptera:Drosophilidae)[J].Journal of Economic Entomology,2018.doi:10.1093/jee/toy304.
[21] GU X,CAI P,YANG Y,et al.The response of four braconid parasitoid species to methyl eugenol:optimization of a biocontrol tactic to suppress Bactrocera dorsalis[J].Biological Control,2018,122:101-108.
[22] ZAR J H.Biostatistical analysis[J].Prentice Hall,1999,18(4):797-799.
[23] 杜家纬.植物—昆虫间的化学通讯及其行为控制[J].植物生理与分子生物学学报,2001,27(3):193-200.
[24] REVADI S,VITAGLIANO S,ROSSI STACCONI M V,et al.Olfactory responses of Drosophila suzukii females to host plant volatiles[J].Physiological Entomology,2015,40(1):54-64.
[25] SCHEIDLER N H,LIU C,HAMBY K A,et al.Volatile codes:correlation of olfactory signals and reception in Drosophila-yeast chemical communication[J].Scientific Reports,2015,5:14 059.
[26] 李为争,胡晶晶,陈汉杰,等.挥发性植物源害虫引诱剂筛选与混配方法的新视角[J].应用昆虫学报,2015,52(5):1 094-1 106.
[27] GREGG P C,DEL SOCORRO A P,HENDERSON G S.Development of a synthetic plant volatile-based attracticide for female noctuid moths.Ⅱ.Bioassays of synthetic plant volatiles as attraction for adults of cotton bollworm,Helicoverpa armigera[J].Australian Journal of Entomology,2010,49(1):21-30.
[28] 林翔云.调香术[M].2版.北京:化学工业出版社,2008.
[29] DEL SOCORRO A P,GREGG P C,ALTER D,et al.Development of a synthetic plant volatile-based attracticide for female noctuid moths.Ⅰ.Potential sources of volatiles attractive to Helicoverpa armigera (Hübner) (Lepidoptera:Noctuidae)[J].Australian Journal of Entomology,2010,49(1):10-20.
[2] 蔡普默,李萍,谢冬生,等.斑翅果蝇Drosophila suzukii Matsumura生物防治研究进展[J].应用昆虫学报,2017,54(5):705-715.
[3] LEE J C,DREVES A J,CAVE A M,et al.Infestation of wild and ornamental noncrop fruits by Drosophila suzukii (Diptera:Drosophilidae)[J].Annals of the Entomological Society of America,2015,108(2):117-129.
[4] ASPLEN M K,ANFORA G,BIONDI A,et al.Invasion biology of spotted wing Drosophila (Drosophila suzukii):a global perspective and future priorities[J].Journal of Pest Science,2015,88(3):469-494.
[5] LIN F J,TSENG H C,LEE W Y.A catalogue of the family Drosophilidae in Taiwan (Diptera)[J].Quarterly Journal of Taiwan Museum,1977,30(3/4):345-372.
[6] 薛万琦,赵建铭.中国蝇类[M].沈阳:辽宁科学技术出版社,1996.
[7] 蔡普默,向候君,仪传冬,等.斑翅果蝇危害健康水果机理研究进展[J].江西农业大学学报,2017,39(2):295-301.
[8] 向候君,蔡普默,张贺贺,等.斑翅果蝇Orco基因的生物信息学分析[J].福建农林大学学报(自然科学版),2018,47(4):391-397.
[9] 杨友莹,池必文,季清娥,等.斑翅果蝇对不同颜色的趋性反应以及田间诱捕器的优化[J].武夷科学,2017,33:35-41.
[10] 李洋洋,王珏,原国辉,等.两种关键花香挥发物对棉铃虫的信息多义性[J].生态学杂志,2014,33(2):340-345.
[11] CHA D H,ADAMS T,ROGG H,et al.Identification and field evaluation of fermentation volatiles from wine and vinegar that mediate attraction of spotted wing drosophila,Drosophila suzukii[J].Journal of Chemical Ecology,2012,38(11):1 419-1 431.
[12] 伍苏然,太红坤,李正跃,等.樱桃果蝇田间诱捕方法比较[J].云南农业大学学报,2007,22(5):776-778.
[13] IGLESIAS L E,NYOIKE T W,LIBURD O E.Effect of trap design,bait type,and age on captures of Drosophila suzukii (Diptera:Drosophilidae) in berry crops[J].Journal of Economic Entomology,2014,107(4):1 508-1 518.
[14] HAMBY K A,BECHER P G.Current knowledge of interactions between Drosophila suzukii and microbes,and their potential utility for pest management[J].Journal of Pest Science,2016,89(3):621-630.
[15] 刘燕,谢冬生,胡纯华,等.β-石竹烯对斑翅果蝇雌成虫行为的影响[J].环境昆虫学报,2018,40(3):684-689.
[16] 刘燕,谢冬生,胡纯华,等.杨梅果实成熟度及挥发物对斑翅果蝇定向行为的影响[J].应用昆虫学报,2017,54(5):716-723.
[17] ABRAHAM J,ZHANG A,ANGELI S,et al.Behavioral and antennal responses of Drosophila suzukii (Diptera:Drosophilidae) to volatiles from fruit extracts[J].Environmental Entomology,2015,44(2):356-367.
[18] MAISTRI S.Drosophila suzukii (Matsumura) (Diptera:Drosophilidae):indagini biomolecolari e sensibilità agli attacchi di diverse cultivar di mirtillo (Vaccinium spp.)[D].Veneto,Italy:Università degli Studi di Padova,2012.
[19] HUANG J,GUT L,GRIESHOP M.Evaluation of food-based attractants for Drosophila suzukii (Diptera:Drosophilidae)[J].Environmental Entomology,2017,46(4):878-884.
[20] CAI P,YI C,ZHANG Q,et al.Evaluation of protein bait manufactured from brewery yeast waste for controlling Drosophila suzukii (Diptera:Drosophilidae)[J].Journal of Economic Entomology,2018.doi:10.1093/jee/toy304.
[21] GU X,CAI P,YANG Y,et al.The response of four braconid parasitoid species to methyl eugenol:optimization of a biocontrol tactic to suppress Bactrocera dorsalis[J].Biological Control,2018,122:101-108.
[22] ZAR J H.Biostatistical analysis[J].Prentice Hall,1999,18(4):797-799.
[23] 杜家纬.植物—昆虫间的化学通讯及其行为控制[J].植物生理与分子生物学学报,2001,27(3):193-200.
[24] REVADI S,VITAGLIANO S,ROSSI STACCONI M V,et al.Olfactory responses of Drosophila suzukii females to host plant volatiles[J].Physiological Entomology,2015,40(1):54-64.
[25] SCHEIDLER N H,LIU C,HAMBY K A,et al.Volatile codes:correlation of olfactory signals and reception in Drosophila-yeast chemical communication[J].Scientific Reports,2015,5:14 059.
[26] 李为争,胡晶晶,陈汉杰,等.挥发性植物源害虫引诱剂筛选与混配方法的新视角[J].应用昆虫学报,2015,52(5):1 094-1 106.
[27] GREGG P C,DEL SOCORRO A P,HENDERSON G S.Development of a synthetic plant volatile-based attracticide for female noctuid moths.Ⅱ.Bioassays of synthetic plant volatiles as attraction for adults of cotton bollworm,Helicoverpa armigera[J].Australian Journal of Entomology,2010,49(1):21-30.
[28] 林翔云.调香术[M].2版.北京:化学工业出版社,2008.
[29] DEL SOCORRO A P,GREGG P C,ALTER D,et al.Development of a synthetic plant volatile-based attracticide for female noctuid moths.Ⅰ.Potential sources of volatiles attractive to Helicoverpa armigera (Hübner) (Lepidoptera:Noctuidae)[J].Australian Journal of Entomology,2010,49(1):10-20.