河北省长针线虫新纪录种松长针线虫Longidorus pinus Xu,Ye,Wang & Zhao的形态与分子鉴定
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摘要
2018年,在河北省保定市园林植物紫荆(Cercis chinensis)根围分离到一种长针线虫。经形态学观察和28S rDNA D2D3区序列分析,将其鉴定为松长针线虫(Longidorus pinus Xu, Ye, Wang&Zhao)。其雌虫主要形态特征为:雌虫体长3 048～3 464μm,唇区明显缢缩,宽9.5～10.5μm,侧器囊袋状,齿尖针长66.0～69.5μm,导环距体前端27.5～30.0μm,尾长31～33μm,短圆锥形,尾长与肛门处体宽比值=1.5～1.6。松长针线虫河北种群28S rDNA D2D3区与GenBank数据库的山西种群进行序列比对,相似性为99.2%～99.9%。松长针线虫是河北省长针线虫新纪录种。紫荆是松长针线虫的新寄主。
A Longidorus species was isolated from Cercis chinensis in Baoding city, Hebei province in 2018.The nematode was identified as Longidorus pinus by using morphological observation and 28 S rDNA D2 D3 sequence analysis. It was characterized by females 3 048-3 464 μm long, lip region distinctly offset from the body contour(9.5-10.5 μm wide), amphidial fovea pocket-shaped, odontostyle 66.0-69.5 μm long, guide ring located 27.5-30.0 μm from anterior end, tail short and conoid(31-33 μm long, and c' = 1.5-1.6. 28 S rDNA D2 D3 sequence of L. pinus in Hebei showed high similarities from 99.2% to 99.9% with those sequences in Shanxi. L. pinus is a new record species of needle nematode in Hebei province. And C. chinensis is a new record host of L. pinus.
A Longidorus species was isolated from Cercis chinensis in Baoding city, Hebei province in 2018.The nematode was identified as Longidorus pinus by using morphological observation and 28 S rDNA D2 D3 sequence analysis. It was characterized by females 3 048-3 464 μm long, lip region distinctly offset from the body contour(9.5-10.5 μm wide), amphidial fovea pocket-shaped, odontostyle 66.0-69.5 μm long, guide ring located 27.5-30.0 μm from anterior end, tail short and conoid(31-33 μm long, and c' = 1.5-1.6. 28 S rDNA D2 D3 sequence of L. pinus in Hebei showed high similarities from 99.2% to 99.9% with those sequences in Shanxi. L. pinus is a new record species of needle nematode in Hebei province. And C. chinensis is a new record host of L. pinus.
引文
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[2]赵文霞,杨宝君.中国植物线虫名录[M].北京:中国林业出版社,2006.
[3]XU Y,YE W,WANG J,et al.Morphological and molecular characterisation of Longidorus pinus sp.n.(Nematoda:Longidoridae)from China and a key to known species of Longidorus in China[J].Nematology,2018,20(7):617-639.
[4]田忠玲,蔡瑞杭,李晓琳,等.中国长针线虫属(线虫门:矛线目)2新记录种记述(英文)[J].浙江大学学报(农业与生命科学版),2017,44(1):31-40.
[5]TAYLOR C E,BROWN D J F.Nematode vectors of plant viruses[M].London:CAB International,1997.
[6]陈其文,张卫东,廖力,等.GBT28086-2011长针线虫属(传毒种类)检疫鉴定方法[S].北京:中国标准出版社,2012.
[7]冯志新.植物线虫学[M].北京:中国农业出版社,2001.
[8]WANG H H,ZHUO K,YE W M,et al.Morphological and molecular charaterisation of Pratylenchus parazeae n.sp.(Nematoda:Pratylenchidae)parasitizing sugarcane in China[J].European Journal of Plant Pathology,2015,143(1):173-191.
[9]DE MAN J G.Die Eingheimischen,frei in der reinen erde und im süssen wasser lebends Nematoden[J].Tijdschr.Ned.Dierk.Ver.,1880,5:1-104.
[10]MUNDO-OCAMPO M,TROCCOLI A,SUBBOTINS A,et al.Synonymy of Afenestrata with Heterodera supported by phylogenetics with molecular and morphological characterisation of H.koreana comb.n.and H.orientalis comb.n.(Tylenchida:Heteroderidae)[J].Nematology,2008,10(5):611-632.
[11]DE LEY P,FELIX M A,FRISSE L M,et al.Molecular and morphological characterisation of two reproductively isolated species with mirror-image anatomy(Nematoda:Cephalobidae)[J].Nematology,1999,1(6):591-612.
[12]PALOMARES-RIUS J E,CANTALAPIEDRA-NAVAR-RETE C,GUTIéRREZ-GUTIéRREZ C,et al.Morphological and molecular characterisation of Paralongidorus plesioepimikis n.sp.(Nematoda:Longidoridae)from southern Spain[J].Nematology,2013,15(3):363-378.
[13]DI BELLO P L,LANEY A G,DRUCIAREK T,et al.Anovel emaravirus is associated with redbud yellow ringspot disease[J].Virus Research,2016,222:41-47.
[14]TAYLOR C E,BROWN D J F.Nematode vectors of plant viruses[M].Wallingford:CAB International,1997.
[15]CRAM M M,FRAEDRICH S W.Biology and management of Longidorus americanus in a southern USAnursery[C]//IUFRO Working Party.The Sixth Meeting of the IUFRO Working Party 7.03.04:Diseases and insects in forest nurseries.Jiloviste:Forestry and Game Management Research Institute Strnady,2007:46-52.
[16]G U T IéR R E Z-G U T IéR R E Z C,C A N TA L A P I E-DRA-NAVARRETE C,MONTES-BORREGO M,et al.Molecular phylogeny of the nematode genus Longidorus(Nematoda:Longidoridae)with description of three new species[J].Zoological Journal of the Linnean Society,2013,167(4):473-500.
[17]ARCHIDONA-YUSTE A,NAVAS-CORTéS J A,CAN-TALAPIEDRA-NAVARRETE C,et al.Unravelling the biodiversity and molecular phylogeny of needle nematodes of the genus Longidorus(Nematoda:Longidoridae)in olive and a description of six new species[J].PLoSOne,2016,11(1):e0147689.
[18]SUBBOTIN S A,STANLEY J D,PLOEG A T,et al.Characterisation of populations of Longidorus orientalis Loof,1982(Nematoda:Dorylaimida)from date palm(Phoenix dactylifera L.)in the USA and other countries and incongruence of phylogenies inferred from ITS1 rRNA and coxIgenes[J].Nematology,2015,17(4):459-477.