反相高效液相色谱法测定治糜康栓中苦参碱和氧化苦参碱的含量
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摘要
目的建立治糜康栓中苦参碱和氧化苦参碱的含量测定方法,以期更全面地控制治糜康栓的质量。方法采用(RP-HPLC法)以十八烷基硅烷键合硅胶(C18)色谱柱(4.6 mm×250 mm,5μm),以乙腈-磷酸盐缓冲液(7:93,磷酸二氢钾1.7 g加500 mL纯水溶解,用磷酸调节pH=3)为流动相,检测波长220 nm,流速1 mL/min,分别对市售治糜康栓与实验室自制治糜康栓中苦参碱及氧化苦参碱的总含量进行测定。结果市售治糜康栓中苦参碱及氧化苦参碱的总含量约为1.52 mg/粒,实验室自制治糜康栓中苦参碱及氧化苦参碱的总含量约为2.53 mg/粒。结论建立的反相高效液相色谱方法适用于治糜康栓中苦参碱及氧化苦参碱的含量测定。
Objective To establish a method for content determination of matrine and oxymatrine in Zhimi Kangshuan,in order to more comprehensive quality control of the Zhimi Kangshuan.Methods Octadecylsilane chemically bonded silica(C18) column(4.6×250 mm,5 μm) was added to the acetonitrile-phosphate buffer as mobile phase(7:93,1.7 g and 500 mL potassium dihydrogen phosphate water solution,adjusted with phosphoric acid pH=3),the detection wavelength was 220 nm,the flow rate was 1 mL/min,and the total content of matrine and oxymatrine was prepared by the preparation of the Chinese Pharmacopoeia and the self-made.Results The total content of matrine and oxymatrine in the market drugs is about 1.52 mg/li.The total content of matrine and oxymatrine in the lab made products is about 2.53 mg/li.Conclusion This paper establishes to be applicable to the determination of matrine and oxymatrine content in Zhimi Kangshuan by reversed phase high performance liquid chromatography.
Objective To establish a method for content determination of matrine and oxymatrine in Zhimi Kangshuan,in order to more comprehensive quality control of the Zhimi Kangshuan.Methods Octadecylsilane chemically bonded silica(C18) column(4.6×250 mm,5 μm) was added to the acetonitrile-phosphate buffer as mobile phase(7:93,1.7 g and 500 mL potassium dihydrogen phosphate water solution,adjusted with phosphoric acid pH=3),the detection wavelength was 220 nm,the flow rate was 1 mL/min,and the total content of matrine and oxymatrine was prepared by the preparation of the Chinese Pharmacopoeia and the self-made.Results The total content of matrine and oxymatrine in the market drugs is about 1.52 mg/li.The total content of matrine and oxymatrine in the lab made products is about 2.53 mg/li.Conclusion This paper establishes to be applicable to the determination of matrine and oxymatrine content in Zhimi Kangshuan by reversed phase high performance liquid chromatography.
引文
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[17]杨志欣,王海威,张文君,等.苦参总黄酮含量测定方法的优化[J].中成药,2017,39(5):946-951.
[18]计曼艳,曾琨,王华.HPLC-DAD法同时测定中药苦参中七种生物碱成分的含量[J].中国药师,2017,20(1):60-63.
[19]付起凤,曹琦,吕邵娃,等.正交法优化苦参中苦参生物碱的超声提取工艺[J].中医药信息,2015,32(1):11-13.
[20]王秀兰,洪宗超,吴松涛,等.高效液相色谱法测定复方苦参汤中苦参碱、氧化苦参碱的含量[J].药物分析与检验,2017,15(10):1426-1429.
[2]李晓燕,赵韶华,等.苦参碱含量测定中色谱条件的改进[J].中成药,2004,26(2):172-173.
[3]罗明,贺平,吴孟超,等.高效毛细管电泳法测定苦参碱和氧化苦参碱[J].中草药,1999,30:(4):26.
[4]刘莹,孟庆妍,翟宏宇.HPLC法同时测定复方苦参肠炎康片中苦参碱和氧化苦参碱的含量[J].长春中医药大学学报,2011,27(5):842-843.
[5]唐坤,李标,夏晨燕,等.反相高效液相色谱测定清益康乳膏中苦参碱的含量[J].时珍国医国药,2007,18(8):1829-1830.
[6]毛丹,陈钶.HPLC法测定苦参软膏中苦参碱、槐定碱和氧化苦参碱[J].中成药,2011,33(9):1531-1534.
[7]张蕾,陈晓辉.RP-HPLC法测定苦参中苦参碱的含量[J].沈阳药科大学学报,2005,22(1):33-35.
[8]吴春红,唐卫文.HPLC法测定苦参药材中苦参碱的含量[J].药物分析杂志,2008,28(7):1147-1149.
[9]时晓亚,方宝霞.高效液相色谱法测定复方苦参子洗液中苦参碱与氧化苦参碱的含量[J].新乡医学院学报,2011,3(28):288-290.
[10]杨文远,杨宁莲.HPLC法同时测定苦豆子中苦参碱与氧化苦参碱[J].宁夏大学学报,1996,17(4):13.
[11]吴迪,梁健.RP-HPLC法同时测定复方苦参注射液中氧化槐果碱、氧化苦参碱和苦参碱的含量[J].沈阳药科大学学报,2006,23(4):220-223.
[12]石猛,莫尚志.醇沉法存在的问题及解决办法[J].中药材,1999,22(6):313-314.
[13]陈宏降,李祥,陈建伟,等.中药三白草地上部位的化学成分研究(Ⅰ)[J].南京中医药大学学报,2009,25(4):286.
[14]邓惠文,欧子强,李元文,等.中药蛇床子中黄酮类成分的研究[J].广州化工,2009,37(6):105-106,114.
[15]孙文基,贾敏鸽.苦参及其复方中苦参碱与氧化苦参碱的转化研究[J].药物分析杂志,2003,23(2):90-93.
[16]江维克,王丽.HPLC法同时测定复方石韦片和苦参药材中苦参碱和氧化苦参碱的含量[J].中成药,2005,27(1):45-48.
[17]杨志欣,王海威,张文君,等.苦参总黄酮含量测定方法的优化[J].中成药,2017,39(5):946-951.
[18]计曼艳,曾琨,王华.HPLC-DAD法同时测定中药苦参中七种生物碱成分的含量[J].中国药师,2017,20(1):60-63.
[19]付起凤,曹琦,吕邵娃,等.正交法优化苦参中苦参生物碱的超声提取工艺[J].中医药信息,2015,32(1):11-13.
[20]王秀兰,洪宗超,吴松涛,等.高效液相色谱法测定复方苦参汤中苦参碱、氧化苦参碱的含量[J].药物分析与检验,2017,15(10):1426-1429.