规模化猪场猪源沙门氏菌的耐药性及相关耐药基因检测
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摘要
为了解贵州省某规模化猪场猪源沙门氏菌分离株的药物敏感性和耐药基因的存在情况,也为进一步研究细菌耐药的分子机制和临床用药提供资料,利用K-B法检测了6株猪源沙门氏菌对15种药物的敏感性,采用PCR方法检测了9种常见耐药基因在分离株中的分布情况。结果显示,所有菌株对青霉素、氨苄西林和吡哌酸的耐药率最高均为100%,对头孢克洛、链霉素和庆大霉素的耐药率最低。从9种常见耐药基因中扩增到3种β-内酰胺类耐药、3种氨基糖苷类、2种氟喹诺酮耐药基因。研究表明,沙门氏菌极易产生耐药性,耐药基因广泛存在于耐药菌株中,但耐药表型与耐药基因之间无绝对相关性。
In order to understand the drug susceptibility and drug resistance genes of Salmonella isolates from a large-scale pig farm in Guizhou Province, to provide information on the molecular mechanism and clinical use of bacterial resistance, six pigs were detected by KB method. The sensitivity of Salmonella to 15 drugs was tested by PCR to detect the distribution of 9 common drug resistance genes in isolates. The results showed that all strains had the highest resistance rate to penicillin, ampicillin and pidamic acid, and the lowest resistance rate to cefaclor, streptomycin and gentamicin. Three kinds of β-lactam resistance, three aminoglycosides and two fluoroquinolone resistance genes were amplified from nine common drug resistance genes. Studies have shown that Salmonella is highly susceptible to drug resistance, and drug resistance genes are widely present in drug-resistant strains, but there is no absolute correlation between drug-resistant phenotypes and drug-resistant genes.
In order to understand the drug susceptibility and drug resistance genes of Salmonella isolates from a large-scale pig farm in Guizhou Province, to provide information on the molecular mechanism and clinical use of bacterial resistance, six pigs were detected by KB method. The sensitivity of Salmonella to 15 drugs was tested by PCR to detect the distribution of 9 common drug resistance genes in isolates. The results showed that all strains had the highest resistance rate to penicillin, ampicillin and pidamic acid, and the lowest resistance rate to cefaclor, streptomycin and gentamicin. Three kinds of β-lactam resistance, three aminoglycosides and two fluoroquinolone resistance genes were amplified from nine common drug resistance genes. Studies have shown that Salmonella is highly susceptible to drug resistance, and drug resistance genes are widely present in drug-resistant strains, but there is no absolute correlation between drug-resistant phenotypes and drug-resistant genes.
引文
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[2]Guerra B,Soto S M,Argüelles J M,et al.Multidrug Resistance Is Mediated by Large Plasmids Carrying a Class 1 Integron in the Emergent Salmonella enterica Serotype[4,5,12:i:-][J].Antimicrobial Agents&Chemotherapy,2001,45(4):1305-1308.
[3]江萍,关茹飞,夏利宁,等.新疆猪源沙门氏菌耐药性及耐药基因检测[J].中国畜牧兽医,2017,44(3):896-903.
[4]潘志明,焦新安,刘学贤,等.鸡白痢沙门氏菌耐药性的变化趋势[J].中国预防兽医学报,1999,21(4):305-307.
[5]Thong K L,Modarressi S.Antimicrobial resistant genes associated with Salmonella from retail meats and street foods[J].Food Research International,2011,44(9):2641-2646.
[6]曹正花,谭艾娟,吕世明,等.贵州省猪源沙门氏菌对β-内酰胺类药耐药性及耐药基因分析[J].中国畜牧兽医,2016,43(7):1737-1742.
[7]刘艳红.沙门氏菌和大肠杆菌耐药性及耐药基因的研究[D].沈阳:沈阳农业大学,2016.