电聚合构建HRP传感器检测有机过氧化物
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摘要
利用电化学还原氧化石墨烯(GO)的方法将还原石墨烯(erGO)沉积在电极表面上,然后电沉积氧化锌纳米棒(ZnO)构成erGO/ZnO复合纳米材料修饰玻碳电极(GCE),最后通过电聚合中性红(NR)电子介体包埋法将辣根过氧化物酶(HRP)固定在GCE/erGO/ZnO表面制得GCE/erGO/ZnO/HRP-PNR(聚中性红)。用SEM和能谱对复合纳米材料进行了表征,通过电化学阻抗法和循环伏安法对修饰电极进行了探究,通过循环伏安法和计时电流法研究了GCE/erGO/ZnO/HRP-PNR对有机过氧化物的催化性能,结果该修饰电极对过氧化氢叔丁基(BHP)和过氧化氢异丙苯(CHP)都具有良好的检测性能。
Electrochemical reduced graphenes(erGO) were electrodeposited onto the electrode surface through the electrochemical reduction of graphite oxides(GO).Then,by the deposition of zinc oxide nanorods(ZnO),erGO/ZnO nanocomposites modified glassy carbon electrode was fabricated.Finally,horseradish peroxidases(HRP) were immobilized on the GCE/erGO/ZnO surface by the method of electropolymerization of neutral red electron mediator to obtain the GCE/erGO/ZnO/HRPPNR(PNR:poly neutral red).SEM and energy spectrum were used to characterize nanocomposties.The modified electrode was studied by the electrochemical impedance spectroscopy and cyclic voltammetry.The catalytic properties of GCE/erGO/ZnO/HRP-PNR towards organic peroxides were studied by electrochemical voltammetry and the chronoamperometry.The modified electrode has good performances for the detection of tert-butyl hydroperoxide(BHP) and cumene hydroperoxide(CHP).
Electrochemical reduced graphenes(erGO) were electrodeposited onto the electrode surface through the electrochemical reduction of graphite oxides(GO).Then,by the deposition of zinc oxide nanorods(ZnO),erGO/ZnO nanocomposites modified glassy carbon electrode was fabricated.Finally,horseradish peroxidases(HRP) were immobilized on the GCE/erGO/ZnO surface by the method of electropolymerization of neutral red electron mediator to obtain the GCE/erGO/ZnO/HRPPNR(PNR:poly neutral red).SEM and energy spectrum were used to characterize nanocomposties.The modified electrode was studied by the electrochemical impedance spectroscopy and cyclic voltammetry.The catalytic properties of GCE/erGO/ZnO/HRP-PNR towards organic peroxides were studied by electrochemical voltammetry and the chronoamperometry.The modified electrode has good performances for the detection of tert-butyl hydroperoxide(BHP) and cumene hydroperoxide(CHP).
引文
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