吩嗪在铜绿假单胞菌去除六价铬中的作用
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摘要
研究了铜绿假单胞菌的吩嗪类分泌物:绿脓菌素(pyocyanine,PYO)和1-羟基吩嗪(1-hydroxyphenazine,POH),在铜绿假单胞菌去除Cr(Ⅵ)中的作用。采用分光光度法和原子吸收光谱法测定样品中Cr(Ⅵ)和总铬在不同时间点的浓度,结果表明,铜绿假单胞菌可吸附并还原Cr(Ⅵ),并且PYO和POH均可加速菌体对Cr(Ⅵ)的还原,随着吩嗪浓度增大,还原速率加快,当PYO或POH的浓度为50μmol/L时,12 h内的Cr(Ⅵ)还原量分别为12 mg/L和16 mg/L。
Two kinds of phenazine,i. e. pyocyanin( PYO) and 1-hydroxyphenazine( POH),were used to study the role of Pseudomonas aeruginosa in Cr( Ⅵ) removal. The concentrations of Cr( Ⅵ) and total Cr were determined by spectrophotometry and atomic absorption spectrometry. The results showed that Pseudomonas aeruginosa reduced Cr( Ⅵ) to Cr( Ⅲ); PYO and POH accelerated Cr( Ⅵ) reduction rate,which was enhanced with an increase in concentration of phenazine. The amounts of reduced Cr( Ⅵ) were 12 and 16 mg / L,respectively within 12 h at 50 μmol / L PYO or POH.
Two kinds of phenazine,i. e. pyocyanin( PYO) and 1-hydroxyphenazine( POH),were used to study the role of Pseudomonas aeruginosa in Cr( Ⅵ) removal. The concentrations of Cr( Ⅵ) and total Cr were determined by spectrophotometry and atomic absorption spectrometry. The results showed that Pseudomonas aeruginosa reduced Cr( Ⅵ) to Cr( Ⅲ); PYO and POH accelerated Cr( Ⅵ) reduction rate,which was enhanced with an increase in concentration of phenazine. The amounts of reduced Cr( Ⅵ) were 12 and 16 mg / L,respectively within 12 h at 50 μmol / L PYO or POH.
引文
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[16]Hernandez M.E.,Kappler A.,Newman D.K.Phenazines and other redox-active antibiotics promote microbial mineral reduction.Applied and Environmental Microbiology,2004,70(2):921-928
[2]Tahri Joutey N.,Bahafid W.,Sayel H.,et al.Hexavalent chromium removal by a novel Serratia proteamaculans isolated from the bank of Sebou River(Morocco).Environmental Science and Pollution Research,2014,21(4):3060-3072
[3]Belchik S.M.,Kennedy D.W.,Dohnalkova A.C.,et al.Extracellular reduction of hexavalent chromium by cytochromes Mtr C and Omc A of Shewanella oneidensis MR-1.Applied and Environmental Microbiology,2011,77(12):4035-4041
[4]Cheung K.H.,Gu J.D.Mechanism of hexavalent chromium detoxification by microorganisms and bioremediation application potential:A review.International Biodeterioration&Biodegradation,2007,59(1):8-15
[5]Salamanca D.,Strunk N.,Engesser K.H.Chromate reduction in anaerobic systems by bacterial strain Pseudomonas aeruginosa CRM100.Chemie Ingenieur Technik,2013,85(10):1575-1580
[6]Xafenias N.,Zhang Yue,Banks C.J.Enhanced performance of hexavalent chromium reducing cathodes in the presence of Shewanella oneidensis MR-1 and lactate.Environmental Science&Technology,2013,47(9):4512-4520
[7]Guo Jianbo,Lian Jing,Xu Zhifang,et al.Reduction of Cr(VI)by Escherichia coli BL21 in the presence of redox mediators.Bioresource Technology,2012,123:713-716
[8]Hong Yiguo,Wu Peng,Li Wenru,et al.Humic analog AQDS and AQS as an electron mediator can enhance chromate reduction by Bacillus sp.strain 3C3.Applied Microbiology and Biotechnology,2012,93(6):2661-2668
[9]Chen Hong,Li Xiaojuan,Xu Zhiwei.Cr(VI)remediation by enriched sediment with anthraquinone-2,6-disulfonate as electron shuttles.Physics and Chemistry of the Earth,Parts A/B/C,2011,36(9-11):451-454
[10]Brose D.A.,James B.R.Oxidation-reduction transformations of chromium in aerobic soils and the role of electron-shuttling quinones.Environmental Science&Technology,2010,44(24):9438-9444
[11]Wang Yun,Kern S.E.,Newman D.K.Endogenous phenazine antibiotics promote anaerobic survival of Pseudomonas aeruginosa via extracellular electron transfer.Journal of Bacteriology,2010,192(1):365-369
[12]Hernandez M.E.,Newman D.K.Extracellular electron transfer.Cellular and Molecular Life Sciences,2001,58(11):1562-1571
[13]Essar D.W.,Eberly L.,Hadero A.,et al.Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa:Interchangeability of the two anthranilate synthases and evolutionary implications.Journal of Bacteriology,1990,172(2):884-900
[14]Ganguli A.,Tripathi A.Bioremediation of toxic chromium from electroplating effluent by chromate-reducing Pseudomonas aeruginosa A2Chr in two bioreactors.Applied Microbiology and Biotechnology,2002,58(3):416-420
[15]Wang Yun,Newman D.K.Redox reactions of phenazine antibiotics with ferric(hydr)oxides and molecular oxygen.Environmental Science&Technology,2008,42(7):2380-2386
[16]Hernandez M.E.,Kappler A.,Newman D.K.Phenazines and other redox-active antibiotics promote microbial mineral reduction.Applied and Environmental Microbiology,2004,70(2):921-928