地衣芽孢杆菌B-1降解西维因的环境条件
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摘要
以地衣芽孢杆菌B-1(Bacillus licheniformis B-1)为材料,采用HPLC测定不同微生物培养体系中西维因(Carbaryl)残留量,并考察菌株B-1降解谱,探究其对西维因的降解特性。结果表明,菌株B-1对西维因的降解率与其生长呈正相关,在LB培养基中培养72 h对100 mg/L西维因的降解率为91.21%。较高的温度和碱性环境有利于西维因的降解,而较高底物质量浓度和盐质量浓度均对菌株B-1的生长及西维因的降解有负面影响;Ca~(2+)、Fe~(2+)、Mn~(2+)、Mg~(2+)对其降解西维因有不同程度的刺激作用,Cu~(2+)则有抑制作用。此外,菌株B-1对20 mg/L的丁硫克百威、叶蝉散和毒死蜱均有不同程度的降解作用。HPLC分析结果表明,初步确定1-萘酚是菌株B-1降解西维因的中间产物,且该菌株在LB培养基中培养72 h对20 mg/L 1-萘酚的降解率为48.70%。可见,菌株B-1能高效降解西维因和其他多类杀虫剂,具有一定的广谱性,可应用于环境修复。
Degradation characteristics of carbaryl by strain Bacillus licheniformis B-1 were studied. Concentrations of carbaryl residue in different microbial culture systems were determined through HPLC,and degradation spectrum of strain B-1 was explored. The results showed that degradation of carbaryl had a positive correlation with the growth of strain B-1. Degradation rate reached up to 91. 21% when the strain was cultured in LB medium with 100 mg/L carbaryl for 72 h. Higher temperatures and alkaline circumstance were advantageous for degradation of carbaryl. Simultaneously,growth of strain B-1 and carbaryl degradation were inhibited with increasing substrate concentrations or salinity. Carbaryl degradation were stimulated when treating with metal ions such as Ca~(2+),Fe~(2+),Mn~(2+),Mg~(2+),whereas inhibited by Cu~(2+). In addition,this strain had degradation effect on 20 mg/L carbosulfan,isoprocarb and chlorpyrifos with different rate. HPLC results showed that 1-naphthol was an intermediate product of carbaryl,and strain B-1 could degrade 48. 70% of 1-naphthol( 20 mg/L) in LB medium after72 h incubation. In conclusion,strain B-1 can efficiently degrade carbaryl and other kinds of insecticides,which shows that strain B-1 possesses a wide-spectrum degrading activity and potential for environmental remediation.
Degradation characteristics of carbaryl by strain Bacillus licheniformis B-1 were studied. Concentrations of carbaryl residue in different microbial culture systems were determined through HPLC,and degradation spectrum of strain B-1 was explored. The results showed that degradation of carbaryl had a positive correlation with the growth of strain B-1. Degradation rate reached up to 91. 21% when the strain was cultured in LB medium with 100 mg/L carbaryl for 72 h. Higher temperatures and alkaline circumstance were advantageous for degradation of carbaryl. Simultaneously,growth of strain B-1 and carbaryl degradation were inhibited with increasing substrate concentrations or salinity. Carbaryl degradation were stimulated when treating with metal ions such as Ca~(2+),Fe~(2+),Mn~(2+),Mg~(2+),whereas inhibited by Cu~(2+). In addition,this strain had degradation effect on 20 mg/L carbosulfan,isoprocarb and chlorpyrifos with different rate. HPLC results showed that 1-naphthol was an intermediate product of carbaryl,and strain B-1 could degrade 48. 70% of 1-naphthol( 20 mg/L) in LB medium after72 h incubation. In conclusion,strain B-1 can efficiently degrade carbaryl and other kinds of insecticides,which shows that strain B-1 possesses a wide-spectrum degrading activity and potential for environmental remediation.
引文
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[24]LIMA M P R,CARDOSO D N,SOARES A M V M,et al.Carbaryl toxicity prediction to soil organisms under high and low temperature regimes[J].Ecotoxicology and Environmental Safety,2015,114:263-272.
[25]董滨,王凤花,林爱军,等.乙草胺降解菌A-3的筛选及其降解特性[J].环境科学,2011,32(2):542-547.
[26]SILVER S,PHUNG L T.A bacterial view of the periodic table:genes and proteins for toxic inorganic ions[J].Journal of Industrial Microbiology and Biotechnology,2005,32(11/12):587-605.
[27]邓维琴,刘书亮,姚开,等.3-苯氧基苯甲酸降解菌Sphingomonas sp.SC-1降解苯酚环境条件及其降解中间产物的研究[J].微生物学通报,2015,42(3):497-503.
[28]UYANIK A,ZDEMIR M.Effect of the environmental temperature on the degradation period of carbaryl[J].Turkish Journal of Agriculture and Forestry,1999,23(6):579-584.
[29]JIRIES A G,AL NASIR F M,BEESE F.Pesticide and heavy metals residue in wastewater,soil and plants in wastewater disposal site near Al-Lajoun Valley,Karak/Jordan[J].Water,Air,&Soil Pollution,2002,133(1):97-107.
[30]TALLUR P N,MEGADI V B,NINNEKAR H Z.Biodegradation of cypermethrin by Micrococcus sp.strain CPN 1[J].Biodegradation,2008,19(1):77-82.
[31]CHEN S H,CHANG C Q,DENG Y Y,et al.Fenpropathrin biodegradation pathway in Bacillus sp.DG-02 and its potential for bioremediation of pyrethroid-contaminated soils[J].Journal of Agricultural and Food Chemistry,2014,62(10):2147-2157.
[32]SINGH B K,WALKER A.Microbial degradation of organophosphorus compounds[J].FEMS Microbiology Reviews,2006,30(3):428-471.
[33]SOGORB M A,VILANOVA E.Enzymes involved in the detoxification of organophosphorus,carbamate and pyrethroid insecticides through hydrolysis[J].Toxicology Letters,2002,128(1):215-228.
[34]ROSS M K,STREIT T M,HERRING K L,et al.Carboxylesterases:dual roles in lipid and pesticide metabolism[J].Journal of Pesticide Science,2010,35(3):257-264.
[2]GUNASEKARA A S,RUBIN A L,GOH K S,et al.Environmental fate and toxicology of carbaryl[M]//WHITACRE D M.Reviews of environmental contamination and toxicology.New York:Springer,2008:95-121.
[3]TOUMI H,BURGA-PEREZ K F,FERARD J F.Acute and chronic ecotoxicity of carbaryl with a battery of aquatic bioassays[J].Journal of Environmental Science and Health,Part B,2016,51(1):57-62.
[4]HAWKER D.Kinetics of carbaryl hydrolysis:An undergraduate environmental chemistry laboratory[J].Journal of Chemical Education,2015,92(9):1531-1535.
[5]MOTTES C,LESUEUR-JANNOYER M,LE BAIL M,et al.Pesticide transfer models in crop and watershed systems:a review[J].Agronomy for Sustainable Development,2014,34(1):229-250.
[6]KATAGI T.Bioconcentration,bioaccumulation,and metabolism of pesticides in aquatic organisms[M]//WHITACRE D M.Reviews of environmental contamination and toxicology.New York:Springer,2010:1-132.
[7]GILLIOM R J,HAMILTON P A.Pesticides in the nation's streams and ground water,1992-2001-a summary[R].Sacramento:U.S.Geological Survey,2006.
[8]SHAMSIPUR M,YAZDANFAR N,GHAMBARIAN M.Combination of solid-phase extraction with dispersive liquid-liquid microextraction followed by GC-MS for determination of pesticide residues from water,milk,honey and fruit juice[J].Food Chemistry,2016,204:289-297.
[9]KHOOBDEL M,SHAYEGHI M,GOLSORKHI S,et al.Effectiveness of ultrasound and ultraviolet irradiation on degradation of carbaryl from aqueous solutions[J].Iranian Journal of ArthropodBorne Diseases,2010,4(1):47.
[10]KONG L J,LEMLEY A T.Effect of nonionic surfactants on the oxidation of carbaryl by anodic Fenton treatment[J].Water Research,2007,41(12):2794-2802.
[11]SWETHA V P,PHALE P S.Metabolism of carbaryl via 1,2-dihydroxynaphthalene by soil isolates Pseudomonas sp.strains C4,C5,and C6[J].Applied and Environmental Microbiology,2005,71(10):5951-5956.
[12]HAYATSU M,HIRANO M,NAGATA T.Involvement of two plasmids in the degradation of carbaryl by Arthrobacter sp.strain RC100[J].Applied and Environmental Microbiology,1999,65(3):1015-1019.
[13]YAN Q X,WANG Y X,LI S P,et al.Sphingobium qiguonii sp.nov.,a carbaryl-degrading bacterium isolated from a wastewater treatment system[J].International Journal of Systematic and Evolutionary Microbiology,2010,60(12):2724-2728.
[14]DODDAMANI H P,NINNEKAR H Z.Biodegradation of carbaryl by a Micrococcus species[J].Current Microbiology,2001,43(1):69-73.
[15]HASHIMOTO M,FUKUI M,HAYANO K,et al.Nucleotide sequence and genetic structure of a novel carbaryl hydrolase gene(ceh A)from Rhizobium sp.strain AC100[J].Applied and Environmental Microbiology,2002,68(3):1220-1227.
[16]ZHANG Q,LIU Y,LIU Y H.Purification and characterization of a novel carbaryl hydrolase from Aspergillus niger PY168[J].FEMS Microbiology Letters,2003,228(1):39-44.
[17]YANG L,CHEN S H,HU M Y,et al.Biodegradation of carbofuran by Pichia anomala strain HQ-C-01 and its application for bioremediation of contaminated soils[J].Biology and Fertility of Soils,2011,47(8):917-923.
[18]MEN J,CHENG F.Biodegradation and growth characteristics of a toluene-degrading strain[J].African Journal of Biotechnology,2011,10(61):13299-13306.
[19]FENNER K,CANONICA S,WACKETT LP,et al.Evaluating pesticide degradation in the environment:blind spots and emerging opportunities[J].Science,2013,341(6147):752-758.
[20]赖文,刘书亮,赵楠,等.氯氰菊酯高效降解菌的筛选鉴定及其降解特性[J].食品科学,2012,33(21):157-163.
[21]LIU K H,KIM J H.In vitro dermal penetration study of carbofuran,carbosulfan,and furathiocarb[J].Archives of Toxicology,2003,77(5):255-260.
[22]HASSANZADEH N,BAHRAMIFAR N,ESMAILI-SARI A.Residue content of carbaryl applied on greenhouse cucumbers and its reduction by duration of a pre-harvest interval and post-harvest household processing[J].Journal of the Science of Food and Agriculture,2010,90(13):2249-2253.
[23]DELORENZO M E,SCOTT G I,ROSS P E.Toxicity of pesticides to aquatic microorganisms:a review[J].Environmental Toxicology and Chemistry,2001,20(1):84-98.
[24]LIMA M P R,CARDOSO D N,SOARES A M V M,et al.Carbaryl toxicity prediction to soil organisms under high and low temperature regimes[J].Ecotoxicology and Environmental Safety,2015,114:263-272.
[25]董滨,王凤花,林爱军,等.乙草胺降解菌A-3的筛选及其降解特性[J].环境科学,2011,32(2):542-547.
[26]SILVER S,PHUNG L T.A bacterial view of the periodic table:genes and proteins for toxic inorganic ions[J].Journal of Industrial Microbiology and Biotechnology,2005,32(11/12):587-605.
[27]邓维琴,刘书亮,姚开,等.3-苯氧基苯甲酸降解菌Sphingomonas sp.SC-1降解苯酚环境条件及其降解中间产物的研究[J].微生物学通报,2015,42(3):497-503.
[28]UYANIK A,ZDEMIR M.Effect of the environmental temperature on the degradation period of carbaryl[J].Turkish Journal of Agriculture and Forestry,1999,23(6):579-584.
[29]JIRIES A G,AL NASIR F M,BEESE F.Pesticide and heavy metals residue in wastewater,soil and plants in wastewater disposal site near Al-Lajoun Valley,Karak/Jordan[J].Water,Air,&Soil Pollution,2002,133(1):97-107.
[30]TALLUR P N,MEGADI V B,NINNEKAR H Z.Biodegradation of cypermethrin by Micrococcus sp.strain CPN 1[J].Biodegradation,2008,19(1):77-82.
[31]CHEN S H,CHANG C Q,DENG Y Y,et al.Fenpropathrin biodegradation pathway in Bacillus sp.DG-02 and its potential for bioremediation of pyrethroid-contaminated soils[J].Journal of Agricultural and Food Chemistry,2014,62(10):2147-2157.
[32]SINGH B K,WALKER A.Microbial degradation of organophosphorus compounds[J].FEMS Microbiology Reviews,2006,30(3):428-471.
[33]SOGORB M A,VILANOVA E.Enzymes involved in the detoxification of organophosphorus,carbamate and pyrethroid insecticides through hydrolysis[J].Toxicology Letters,2002,128(1):215-228.
[34]ROSS M K,STREIT T M,HERRING K L,et al.Carboxylesterases:dual roles in lipid and pesticide metabolism[J].Journal of Pesticide Science,2010,35(3):257-264.