基于超滤-络合萃取技术的甘草酸制备工艺研究
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摘要
目的建立一种采用超滤-络合萃取技术制备甘草酸的工艺。方法考察pH值(p H 4～8)对超滤液中甘草酸萃取率的影响;采用正交试验优选络合萃取甘草酸的工艺条件;通过考察反萃取剂种类及浓度,确定甘草酸的反萃取工艺条件。结果所得甘草酸最佳络合萃取工艺条件为超滤液pH值应调至2、萃取剂为三烷基氧化膦(TRPO)-磺化煤油(5∶95)、有机相与水相体积比为1∶1,甘草酸平均萃取率达到99.2%。甘草酸最佳反萃取工艺条件为22.5 mmol/L NaOH水溶液为反萃取剂、有机相与反萃取剂体积比1∶1,甘草酸单次反萃取率达到98.8%,甘草酸总转移率为98.1%。结论超滤-络合萃取技术可作为甘草酸制备的一种新工艺。
Objective To establish a technique for preparing glycyrrhizin acid(GA) by ultrafiltration-complexation extraction.Methods The effect of pH on the extraction rate of GA from ultrafiltrate was investigated in the range of 4—8; The orthogonal test was used to optimize the technological conditions for complexation extraction of GA; The extraction conditions of GA were determined by investigating the type and concentration of the extractant. Results The optimum extraction conditions for GA were as follows: pH value of Glycyrrhiza uralensis ultrafiltrate was adjusted to 2, TRPO-sulfonated kerosene(5∶95, volume percent), volume ratio of organic phase to aqueous phase was 1∶1, and average extraction rate of GA reached 99.2%. The best back extraction conditions for GA were as follows: 22.5 mmol/L NaOH aqueous solution was used as the stripping agent, the volume ratio of organic phase to stripping agent was 1∶1, the single back extraction rate of GA reached 98.8%, and the total transfer rate of GA was 98.1%.Conclusion Ultrafiltration-complexation extraction technology can be used as a new process for the preparation of GA.
Objective To establish a technique for preparing glycyrrhizin acid(GA) by ultrafiltration-complexation extraction.Methods The effect of pH on the extraction rate of GA from ultrafiltrate was investigated in the range of 4—8; The orthogonal test was used to optimize the technological conditions for complexation extraction of GA; The extraction conditions of GA were determined by investigating the type and concentration of the extractant. Results The optimum extraction conditions for GA were as follows: pH value of Glycyrrhiza uralensis ultrafiltrate was adjusted to 2, TRPO-sulfonated kerosene(5∶95, volume percent), volume ratio of organic phase to aqueous phase was 1∶1, and average extraction rate of GA reached 99.2%. The best back extraction conditions for GA were as follows: 22.5 mmol/L NaOH aqueous solution was used as the stripping agent, the volume ratio of organic phase to stripping agent was 1∶1, the single back extraction rate of GA reached 98.8%, and the total transfer rate of GA was 98.1%.Conclusion Ultrafiltration-complexation extraction technology can be used as a new process for the preparation of GA.
引文
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[3]田庆来,官月平,张波,等.甘草有效成分的药理作用研究进展[J].天然产物研究与开发,2006,18(2):343-347.
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[5]孙波,李小芹,周方.甘草酸对幼鼠实验性结肠炎的治疗作用及其机制研究[J].现代药物与临床,2018,33(10):2471-2476.
[6]黄群荣,马哲.甘草酸的药理作用研究进展[J].药物评价研究,2011,34(5):384-387.
[7]韩瑶聃,王彬,王政雨,等.甘草酸药理作用的研究进展[J].中国新药杂志,2012,27(21):81-82.
[8]王肖娜,金玉姬,刘洋,等.甘草甜素的应用及研究现状[J].吉林医药学院学报,2014,35(2):144-147.
[9]李玉山.甘草酸提取纯化工艺的研究进展[J].化学试剂,2016,38(5):428-432.
[10]冯月,吴文夫,魏建华,等.甘草酸及甘草苷的提取纯化方法和药理作用研究进展[J].人参研究,2012,24(3):46-50.
[11]赵祎镭,师清芝,唐星.甘草中甘草酸和甘草苷的提取纯化工艺研究[J].中国药房,2009,20(6):426-429.
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[15]戴猷元,秦炜,张瑾.有机物络合萃取化学[M].北京:化学工业出版社,2015.
[16]韩海滨,段泽敏,王贤萍,等.络合萃取技术及其在生物碱分离中的应用[J].中国农学通报,2009,25(2):39-42.
[17]田庆来,谢渝春,张波,等.溶剂萃取法分离水溶性甘草黄酮[J].过程工程学报,2007,7(3):496-500.