基于种苗表型初步构建中国樟树核心种质
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摘要
樟树是我国重要的经济、用材和绿化树种。构建中国樟树核心种质,不仅能准确快速挖掘优异基因源,而且能减少工作量、提高育种效率。以樟树种质资源库中的872份樟树种质资源为材料,根据种源分成45个组,基于种子大小、千粒质量、苗高和地径4个表型性状数据,构建了217份中国樟树核心种质。利用不同性状的多样性指数t检验、均值t检验、方差F检验、均值、极差、变异系数、表型方差、表型频率方差、表型保留比例、变异系数变化率、极差符合率等11个参数和主成分分析进行核心种质代表性检验和评价。结果表明,所构建的中国樟树核心种质能够较好的代表全部种质的遗传多样性。
Camphor tree(Cinnamomum camphora)is an important tree with economic,commercial and or?namental value. The construction of camphor tree core collection in China will not only excavate excellent gene resources accurately and rapidly,but also improve breeding efficiency. A total of 872 germ plasm collections of camphor were sampled and divided into 45 groups based on the provenance. The resultant core collection in?cluding 217 accessions was constructed with 4 traits including seed size and weight,seedling height and ground diameter. Furthermore the representativeness between total collections and core collection was accessed by the t test of diversity index,t test of average,F test of variance,average,range,coefficient of variation,phenotypic variance,phenotypic frequency variance,ratio of phenotype retained,changeable rate of coefficient of variation,coincidence rate of range and principal component analysis. The results showed that the selected core collection could represent the genetic diversity of total collections.
Camphor tree(Cinnamomum camphora)is an important tree with economic,commercial and or?namental value. The construction of camphor tree core collection in China will not only excavate excellent gene resources accurately and rapidly,but also improve breeding efficiency. A total of 872 germ plasm collections of camphor were sampled and divided into 45 groups based on the provenance. The resultant core collection in?cluding 217 accessions was constructed with 4 traits including seed size and weight,seedling height and ground diameter. Furthermore the representativeness between total collections and core collection was accessed by the t test of diversity index,t test of average,F test of variance,average,range,coefficient of variation,phenotypic variance,phenotypic frequency variance,ratio of phenotype retained,changeable rate of coefficient of variation,coincidence rate of range and principal component analysis. The results showed that the selected core collection could represent the genetic diversity of total collections.
引文
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[12]毛钧,刘新龙,苏火生,等.基于表型与分子数据的斑茅核心种质构建[J].植物遗传资源学报,2016,17(4):607-615.Mao J,Liu X L,Su H S,et al.Constructing core collection of Saccharum arundinaceum L.based on phenotype and molecularmarkers,Zhiwu Ziyuan Xuebao[J].Journal of Plant Genetic Resources,2016,17(4):607-615.
[13]徐宁,程须珍,王素华,等.以地理来源分组和利用表型数据构建中国小豆核心种质[J].作物学报,2008,34(8):1366-1373.Xu N,Chen X Z,Wang S H,et al.Establishment of an adzuki bean(Vigna angularis)core collection based on geographicaldistribution and phenotypic data in China[J].Acta Agronomica Sinica,2008,34(8):1366-1373.
[14]刘德浩,张方秋,张卫华.基于地理种源分组和表型数据构建尾叶桉核心种质[J].西南林业大学学报,2013,33(5):1-8.Liu D H,Zhang F Q,Zhang W H,et al.Establishment of eucalyptus urophylla core collection based on geographical distribu?tion and phenotypic data[J].Journal of Southwest Forestry University,2013,33(5):1-8.
[15]钟永达,李彦强,刘立盘,等.基于扫描仪和ImageJ软件的香樟种子大小的测量及其应用[J].西南林业大学学报,2017,37(6):48-53.Zhong Y D,Li Y Q,Liu L P,et al.Application of scanner and ImageJ analysis software in measuring seed size of Cinnamo?mum camphora[J].Journal of Southwest Forestry University,2017,37(6):48-53.
[16]钟永达,周燕玲,李彦强,等.基于ImageJ软件的香樟千粒重的测量及其应用[J].安徽农学通报,2018,24(10):82-85.Zhong Y D,Zhou Y L,Li Y Q,et al.Application of ImageJ analysis software in measuring 1000-seed weight of Cinnamomumcamphora[J].Anhui Agricultural Science Bulletin,2018,24(10):82-85.
[17]Hu J,Zhu J,Xu H M.Methods of constructing core collections by stepwise clustering with three sampling strategies based onthe genotypic values of crops[J].Theoretical and Applied Genetics,2000,101(1-2):264-268.
[18]顾万春.中国林木种质资源保存、研究与对策[C].全国生物多样性保护与持续利用研讨会,2002.Gu W C.The present situation and strategy of forest tree germplasm resources conservation and research in China[C].Na?tional Symposium on Biodiversity Conservation and Sustainable Use,2002.
[19]姚小华.樟树遗传变异与选择的研究[D].长沙:中南林学院,2002.Yao X H.Study on genetic variation and selection of Cinnamomum camphora[D].Changsha:Central-South Forestry Univer?sity,2002.
[20]蔡燕灵,曾令海,连辉明,等.樟树局部种源早期选择研究[J],广东林业科技,2013,29(3):7-12.Cai Y L,Zeng L H,Lian H M,et al.Early selection of local provenance of Cinnamomum camphora in Guangdong province[J].Guangdong Forestry Science and Technology,2013,29(3):7-12.
[21]邹智谨.香樟不同家系生长特性与初步选择的研究[D].福州:福建农林大学,2016.Zhou Z J.The research of camphor’s different family growth characterristics and seeds selection[D].Fuzhou:Fujian Agricul?ture and Forestry University,2016.
[22]许敏铭.香樟遗传变异与良种选择研究[D].福州:福建农林大学,2015.Xu M M.The research of camphor′s genetic variation and seeds selection[D].Fuzhou:Fujian Agriculture and Forestry Uni?versity,2015.
[23]王年金,张盛剿,钱小娟,等.樟树地理种源幼林期生长性状差异分析及优良种源初步选择[J].浙江林业科技,2009,29(4):69-72.Wang N J,Zhang S C,Qian X J,et al.Analysis on growth traits of young Cinnamomun camphora with different provenancesand optimal selection[J].Journal of Zhejiang Forestry Science and Technology,2009,29(4):69-72.
[24]李芳,金志农,黎祖尧,等.樟树种子性状及对生境因子的响应[J].江西农业大学学报,2017,39(1):92-100.Li F,Jin Z N,Li Z R,et al.Seeds properties of Cinnamomum camphora and their response towards environmental factors[J].Acta Agriculturae Universitatis Jiangxiensis,2017,39(1):92-100.
[25]赵冰,张启翔.中国蜡梅种质资源核心种质的初步构建[J].北京林业大学学报,2007,29(S1):16-21.Zhao B,Zhang Q Q.Preliminary construction of the core germplasm of Chimonanthus praecox in China[J].Journal of BeijingForestry University,2007,29(S1):16-21.
[26]徐海明,邱英雄,胡晋,等.不同遗传距离聚类和抽样方法构建作物核心种质的比较[J].作物学报,2004,30(9):932-936.Xu H M,Qiu Y X,Hu J,et al.Methods of constructing core collection of crop germplasm by comparing different genetic dis?tances,cluster methods and sampling strategies[J].Acta Agronomica Sinica,2004,30(9):932-936.
[27]张洪亮,李自超,曹永生,等.表型水平上检验水稻核心种质的参数比较[J].作物学报,2003,29(2):252-257.Zhang H L,Li Z C,Cao Y S,et al.Comparison of parameters for testing the rice core collection in phenotype[J].Acta Agro?nomica Sinica,2003,29(2):252-257.
[28]刘遵春,刘大亮,崔美,等.整合农艺性状和分子标记数据构建新疆野苹果核心种质[J].园艺学报,2012,39(6):1045-1054.Liu Z C,Liu D L,Cui M,et al.Combining agronomic traits and molecular marker data for constructing Malus sieversii corecollection[J].Acta Horticulturae Sinica,2012,39(6):1045-1054.
[29]李秀兰,贾继文,王军辉,等.灰楸形态多样性分析及核心种质初步构建[J].植物遗传资源学报,2013(2),14:243-248.Liu X L,Jia J W,Wang J H,et al.Morphological diversity analysis and preliminary construction of core collection of Catalpafargesii bureau[J].Journal of Plant Genetic Resources,2013,14(2):243-248.
[30]刘鑫铭,刘崇怀,樊秀彩,等.葡萄种质资源初级核心群的构建[J].植物遗传资源学报,2012,13(1):72-76.Liu X M,Liu C H,Fan X C,et al.Construction of primary core collection of grape genetic resources[J].Journal of Plant Ge?netic Resources,2012,13(1):72-76.
[2]黎祖尧,陈尚钘.江西樟树[M].南昌:江西科学技术出版社,2015.Li Z Y,Chen S X. Jiangxi camphor trees[M].Nanchang:Jiangxi Science and Technology Press,2015.
[3]钟永达,胡晓健,余发新.香樟的分子生物学研究进展[J].江西科学,2015,33(2):184-187.Zhong Y D,Hu X J,Yu F X.Molecular biology applied in Cinnamomum camphora[J].Jiangxi Science,2015,33(2):184-187.
[4]Zhou Y,Yan W.Conservation and applications of camphor tree(Cinnamomum camphora)in China:ethnobotany and genet?ic resources[J].Genetic Resources and Crop Evolution,2015,63(6):1049-1061.
[5]Frankle O H.Genetic perspectives of germplasm conseBrvation[M].Cambridge,UK:Cambridge University Press,1984:161-170.
[6]Brown A H D.Core collections:a practical approach to genetic resources management[J].Genome,1989,31(2):818-824.
[7]代攀虹,孙君灵,贾银华,等.利用表型数据构建陆地棉核心种质[J].植物遗传资源学报,2016,17(6):961-968.Dai P H,Sun J L,Jia Y H,et al.Construction of core collection of upland cotton based on phenotypic data[J].Journal ofPlant Genetic Resources,2016,17(6):961-968.
[8]刘娟,廖康,曹倩,等.利用表型性状构建新疆野杏种质资源核心种质[J].果树学报,2015,32(5):787-796.Liu J,Liao K,Cao Q,et al.Establishment of wild apricot core collection based on phenotypic characters[J].Journal of FruitScience,2015,32(5):787-796.
[9]杨汉波,张蕊,王帮顺,等.基于SSR标记的木荷核心种质构建[J].林业科学,2017,53(6):37-46.Yang H B,Zhang R,Wang B S,et al.Construction of core collection of schima superba based on ssr molecular markers[J].Scientia Silvae Sinicae,2017,53(6):37-46.
[10]De Cristo-Araujo M,Rodrigues D P,Astolfi S,et al.Peach palm core collection in Brazilian amazonia[J].Crop Breeding&Applied Biotechnology,2015,15(1):18-25.
[11]王红霞,赵书岗,高仪,等.基于AFLP分子标记的核桃核心种质的构建[J].中国农业科学,2013,46(23):4985-4995.Wang H X,Zhao S G,Gao Y,et al.A construction of the core collection of Juglans regia L.based on AFLP molecular markers[J].Scientia Agricultura Sinica,2013,46(23):4985-4995.
[12]毛钧,刘新龙,苏火生,等.基于表型与分子数据的斑茅核心种质构建[J].植物遗传资源学报,2016,17(4):607-615.Mao J,Liu X L,Su H S,et al.Constructing core collection of Saccharum arundinaceum L.based on phenotype and molecularmarkers,Zhiwu Ziyuan Xuebao[J].Journal of Plant Genetic Resources,2016,17(4):607-615.
[13]徐宁,程须珍,王素华,等.以地理来源分组和利用表型数据构建中国小豆核心种质[J].作物学报,2008,34(8):1366-1373.Xu N,Chen X Z,Wang S H,et al.Establishment of an adzuki bean(Vigna angularis)core collection based on geographicaldistribution and phenotypic data in China[J].Acta Agronomica Sinica,2008,34(8):1366-1373.
[14]刘德浩,张方秋,张卫华.基于地理种源分组和表型数据构建尾叶桉核心种质[J].西南林业大学学报,2013,33(5):1-8.Liu D H,Zhang F Q,Zhang W H,et al.Establishment of eucalyptus urophylla core collection based on geographical distribu?tion and phenotypic data[J].Journal of Southwest Forestry University,2013,33(5):1-8.
[15]钟永达,李彦强,刘立盘,等.基于扫描仪和ImageJ软件的香樟种子大小的测量及其应用[J].西南林业大学学报,2017,37(6):48-53.Zhong Y D,Li Y Q,Liu L P,et al.Application of scanner and ImageJ analysis software in measuring seed size of Cinnamo?mum camphora[J].Journal of Southwest Forestry University,2017,37(6):48-53.
[16]钟永达,周燕玲,李彦强,等.基于ImageJ软件的香樟千粒重的测量及其应用[J].安徽农学通报,2018,24(10):82-85.Zhong Y D,Zhou Y L,Li Y Q,et al.Application of ImageJ analysis software in measuring 1000-seed weight of Cinnamomumcamphora[J].Anhui Agricultural Science Bulletin,2018,24(10):82-85.
[17]Hu J,Zhu J,Xu H M.Methods of constructing core collections by stepwise clustering with three sampling strategies based onthe genotypic values of crops[J].Theoretical and Applied Genetics,2000,101(1-2):264-268.
[18]顾万春.中国林木种质资源保存、研究与对策[C].全国生物多样性保护与持续利用研讨会,2002.Gu W C.The present situation and strategy of forest tree germplasm resources conservation and research in China[C].Na?tional Symposium on Biodiversity Conservation and Sustainable Use,2002.
[19]姚小华.樟树遗传变异与选择的研究[D].长沙:中南林学院,2002.Yao X H.Study on genetic variation and selection of Cinnamomum camphora[D].Changsha:Central-South Forestry Univer?sity,2002.
[20]蔡燕灵,曾令海,连辉明,等.樟树局部种源早期选择研究[J],广东林业科技,2013,29(3):7-12.Cai Y L,Zeng L H,Lian H M,et al.Early selection of local provenance of Cinnamomum camphora in Guangdong province[J].Guangdong Forestry Science and Technology,2013,29(3):7-12.
[21]邹智谨.香樟不同家系生长特性与初步选择的研究[D].福州:福建农林大学,2016.Zhou Z J.The research of camphor’s different family growth characterristics and seeds selection[D].Fuzhou:Fujian Agricul?ture and Forestry University,2016.
[22]许敏铭.香樟遗传变异与良种选择研究[D].福州:福建农林大学,2015.Xu M M.The research of camphor′s genetic variation and seeds selection[D].Fuzhou:Fujian Agriculture and Forestry Uni?versity,2015.
[23]王年金,张盛剿,钱小娟,等.樟树地理种源幼林期生长性状差异分析及优良种源初步选择[J].浙江林业科技,2009,29(4):69-72.Wang N J,Zhang S C,Qian X J,et al.Analysis on growth traits of young Cinnamomun camphora with different provenancesand optimal selection[J].Journal of Zhejiang Forestry Science and Technology,2009,29(4):69-72.
[24]李芳,金志农,黎祖尧,等.樟树种子性状及对生境因子的响应[J].江西农业大学学报,2017,39(1):92-100.Li F,Jin Z N,Li Z R,et al.Seeds properties of Cinnamomum camphora and their response towards environmental factors[J].Acta Agriculturae Universitatis Jiangxiensis,2017,39(1):92-100.
[25]赵冰,张启翔.中国蜡梅种质资源核心种质的初步构建[J].北京林业大学学报,2007,29(S1):16-21.Zhao B,Zhang Q Q.Preliminary construction of the core germplasm of Chimonanthus praecox in China[J].Journal of BeijingForestry University,2007,29(S1):16-21.
[26]徐海明,邱英雄,胡晋,等.不同遗传距离聚类和抽样方法构建作物核心种质的比较[J].作物学报,2004,30(9):932-936.Xu H M,Qiu Y X,Hu J,et al.Methods of constructing core collection of crop germplasm by comparing different genetic dis?tances,cluster methods and sampling strategies[J].Acta Agronomica Sinica,2004,30(9):932-936.
[27]张洪亮,李自超,曹永生,等.表型水平上检验水稻核心种质的参数比较[J].作物学报,2003,29(2):252-257.Zhang H L,Li Z C,Cao Y S,et al.Comparison of parameters for testing the rice core collection in phenotype[J].Acta Agro?nomica Sinica,2003,29(2):252-257.
[28]刘遵春,刘大亮,崔美,等.整合农艺性状和分子标记数据构建新疆野苹果核心种质[J].园艺学报,2012,39(6):1045-1054.Liu Z C,Liu D L,Cui M,et al.Combining agronomic traits and molecular marker data for constructing Malus sieversii corecollection[J].Acta Horticulturae Sinica,2012,39(6):1045-1054.
[29]李秀兰,贾继文,王军辉,等.灰楸形态多样性分析及核心种质初步构建[J].植物遗传资源学报,2013(2),14:243-248.Liu X L,Jia J W,Wang J H,et al.Morphological diversity analysis and preliminary construction of core collection of Catalpafargesii bureau[J].Journal of Plant Genetic Resources,2013,14(2):243-248.
[30]刘鑫铭,刘崇怀,樊秀彩,等.葡萄种质资源初级核心群的构建[J].植物遗传资源学报,2012,13(1):72-76.Liu X M,Liu C H,Fan X C,et al.Construction of primary core collection of grape genetic resources[J].Journal of Plant Ge?netic Resources,2012,13(1):72-76.