响应面法优化云南宣木瓜齐墩果酸和熊果酸的提取工艺研究
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摘要
探索超声辅助提取宣木瓜齐墩果酸和熊果酸的工艺优化研究。在单因素试验的基础上,选取对提取工艺影响较大的乙醇浓度、超声时间和超声功率3个因素,利用Box-Benhnken中心组合原理进行三因素三水平试验,并通过响应面分析法优化超声波辅助提取宣木瓜中齐墩果酸和熊果酸的工艺参数。结果表明最优工艺条件为:乙醇浓度95%,超声时间3.8 min、超声功率440 W,齐墩果酸和熊果酸的总得率为4.03 mg/g,与理论基本相符。因此,超声辅助提取法能快速有效地提取宣木瓜中的齐墩果酸和熊果酸。
The extraction technology of oleanolic acid and ursolic acid from Chaenomeles speciosa by ultrasonic-assisted extraction was studied. Ethanol concentration,ultrasonic time and ultrasonic power were selected as factors based on single-factor test and a three-factors-three-levels experiment design had been developed by Box-Benhnken central composite design method. The extraction technology conditions of oleanolic acid and ursolic acid from Chaenomeles speciosa by ultrasonic-assisted extraction was optimized by response surface analysis experiments. The results showed the optimal conditions were that :concentration of ethanol 95 %,time 3.8 min,ultrasonic power 440 W. Under the optimum conditions,the actual total yield of oleanolic acid and ursolic acid was 4.03 mg/g,which matched with the predicted yield. Oleanolic acid and ursolic acid from Chaenomeles speciosa could be extracted quickly and efficiently by ultrasonic-assisted extraction.
The extraction technology of oleanolic acid and ursolic acid from Chaenomeles speciosa by ultrasonic-assisted extraction was studied. Ethanol concentration,ultrasonic time and ultrasonic power were selected as factors based on single-factor test and a three-factors-three-levels experiment design had been developed by Box-Benhnken central composite design method. The extraction technology conditions of oleanolic acid and ursolic acid from Chaenomeles speciosa by ultrasonic-assisted extraction was optimized by response surface analysis experiments. The results showed the optimal conditions were that :concentration of ethanol 95 %,time 3.8 min,ultrasonic power 440 W. Under the optimum conditions,the actual total yield of oleanolic acid and ursolic acid was 4.03 mg/g,which matched with the predicted yield. Oleanolic acid and ursolic acid from Chaenomeles speciosa could be extracted quickly and efficiently by ultrasonic-assisted extraction.
引文
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[5]孙协军,刘羽纯,潘龙飞,等.山楂中齐墩果酸和熊果酸超声提取工艺研究[J].食品工业科技,2015,36(14):321-326
[6]张红玉,王成章,张宇思,等.超声波提取牡丹籽壳多酚工艺响应面法优化及抗氧化性研究[J].中国油脂,2015,40(6):90-94
[7]李会珍,张红娇,程翀,等.紫苏甾醇超声波辅助提取工艺及其抗氧化性研究[J].中国油脂,2016,41(8):78-82
[8]张海容,史振华.响应面法优化超声波辅助提取沙棘籽粕中黄酮工艺[J].中国油脂,2017,42(3):117-121
[9]高锦鸿,梅鸿献,汪学德,等.芝麻中芝麻素酚三糖苷超声波辅助提取工艺研究[J].中国油脂,2016,41(2):97-100
[10]陈程,王海坤,张存劳,等.响应面法优化超声辅助提取牡丹籽粕中多酚工艺[J].中国油脂,2017,42(3):127-130
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[13]王蓉,邹时英,李艳清,等.反相高效液相色谱法同时测定车前草中熊果酸和齐墩果酸[J].光谱实验室,2011,28(4):1922-1925