“春七”和“冬七”中17种氨基酸的比较研究
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摘要
目的比较分析传统意义上"春七"与"冬七"中氨基酸含量差异,为明确两种采收方法对三七药材质量的影响提供依据。方法采用高效液相色谱法测定三七不同部位的氨基酸含量,利用SAS 9.4软件进行数据分析。结果总氨基酸含量顺序为"春七"剪口>"冬七"支根>"冬七"剪口>"春七"支根>"冬七"主根>"春七"主根;不同采收方法下,三七同一部位有9种氨基酸存在含量差异;同种采收方法下,三七不同部位氨基酸含量无明显差异;"春七"和"冬七"各部位必需氨基酸以及药用氨基酸占比无明显差异。结论文山产区"春七"和"冬七"仅在部分种类氨基酸含量存在差异性,故而推断氨基酸含量可能不是影响两种三七质量的主要原因,文山地区"春七"和"冬七"的差异性还需在其他方面进行深入挖掘。
Objective To provide evidence for the impact of the two methods of harvesting on Panax notoginseng, the author analyzing 17 amino acids in "Chunqi" and "Dongqi". Methods The amino acid composition in Panax notoginseng are measured by HPLC, and the datas are measured by SAS 9.4.Results The content order of total amino acids are "Chunqi" rootlet > "Dongqi" Rhizome > "Dongqi" rootlet > "Chunqi" Rhizome > "Dongqi" root > "Chunqi" root. Under different harvesting methods, there are 9 kinds of amino acids in the same part of Panax notoginseng. Under the same harvesting method, there is no significant difference in amino acid content in different parts of Panax notoginseng. And there is no significant difference in essential amino acids and nonssential amino acids between "Chunqi" and "Dongqi". Conclusion There is no significant difference in total amino acids, essential amino acids and nonssential amino acids of Panax notoginseng at Wenshan. so it is concluded that the amino acid content may not be the main reason for the quality of the two Panax notoginseng. And the differences between "Chunqi" and "Dongqi" still need to dig deeper in other areas.
Objective To provide evidence for the impact of the two methods of harvesting on Panax notoginseng, the author analyzing 17 amino acids in "Chunqi" and "Dongqi". Methods The amino acid composition in Panax notoginseng are measured by HPLC, and the datas are measured by SAS 9.4.Results The content order of total amino acids are "Chunqi" rootlet > "Dongqi" Rhizome > "Dongqi" rootlet > "Chunqi" Rhizome > "Dongqi" root > "Chunqi" root. Under different harvesting methods, there are 9 kinds of amino acids in the same part of Panax notoginseng. Under the same harvesting method, there is no significant difference in amino acid content in different parts of Panax notoginseng. And there is no significant difference in essential amino acids and nonssential amino acids between "Chunqi" and "Dongqi". Conclusion There is no significant difference in total amino acids, essential amino acids and nonssential amino acids of Panax notoginseng at Wenshan. so it is concluded that the amino acid content may not be the main reason for the quality of the two Panax notoginseng. And the differences between "Chunqi" and "Dongqi" still need to dig deeper in other areas.
引文
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[11]赵薇,徐香琴,黄松,等.三七与三七药渣中游离氨基酸的含量比较及氨基酸提取工艺的研究[J].北方药学,2016,13(8):97-99.
[12]郭新苗,郭庆梅,周凤琴.不同采摘期金银花中氨基酸含量的PITC柱前衍生HPLC法测定[J].时珍国医国药,2014,25(10):2501-2504.
[13]吴有锋,谭亮,沈建伟,等.柴达木枸杞中17种氨基酸的测定与分析[J].食品工业科技,2017,38(1):281-286.
[14]尹辉.三七化学成分研究概况[J].齐齐哈尔医学院学报,2015,36(20):3068-3069.
[15]郝庆秀,金艳,刘大会,等.不同产地三七栽培加工技术调查[J].中国现代中药,2014,16(2):123-129.
[16]孙大雨,王德民,王彦波.不同季节及用药部位三七药材的质量分析[J].黑龙江医药,2014,27(3):634-635.
[2]Huang,X P,Chen Tan H,Chen B Y,et al.Combination of Total Astragalus Extract and Total Panax Notoginseng Saponins Strengthened the Protective Effects on Brain Damage through Improving Energy Metabolism and Inhibiting Apoptosis after Cerebral Ischemia-Reperfusion in Mice[J].中国结合医学杂志(英文版),2017,23(6):445-452.
[3]苏萍,王蕾,杜仕静,等.三七皂苷对神经系统疾病药理作用机制研究进展[J].中国中药杂志,2014,39(23):4516-4521.
[4]钟晓凤.三七的药理作用及其临床应用研究[J].中医临床研究,2015,7(6):116-117.
[5]居乃香,孙静.三七药理作用的研究进展[J].北方药学,2014,11(11):90-91.
[6]国家医药管理局,卫生部.七十六种药材商品规格标准[S].1984:15.
[7]刘大会,徐娜,郭兰萍,等.三七药材质量特征和商品规格等级标准研究[J].中国中药杂志,2016,41(5):776-785.
[8]高爱红,王春海,刘双霞.三七的质量评价与鉴别[J].河北中医,2004,26(9):720.
[9]孙大雨,王德民,王彦波.不同季节及用药部位三七药材的质量分析[J].黑龙江医药,2014,27(3):634-635.
[10]于永辉,籍国霞,臧恒昌.中药中氨基酸分析测定技术研究进展[J].食品与药品,2014,16(5):371-373.
[11]赵薇,徐香琴,黄松,等.三七与三七药渣中游离氨基酸的含量比较及氨基酸提取工艺的研究[J].北方药学,2016,13(8):97-99.
[12]郭新苗,郭庆梅,周凤琴.不同采摘期金银花中氨基酸含量的PITC柱前衍生HPLC法测定[J].时珍国医国药,2014,25(10):2501-2504.
[13]吴有锋,谭亮,沈建伟,等.柴达木枸杞中17种氨基酸的测定与分析[J].食品工业科技,2017,38(1):281-286.
[14]尹辉.三七化学成分研究概况[J].齐齐哈尔医学院学报,2015,36(20):3068-3069.
[15]郝庆秀,金艳,刘大会,等.不同产地三七栽培加工技术调查[J].中国现代中药,2014,16(2):123-129.
[16]孙大雨,王德民,王彦波.不同季节及用药部位三七药材的质量分析[J].黑龙江医药,2014,27(3):634-635.