不同压力高压氧对脑出血大鼠Bcl-2/Bax比值的影响
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摘要
目的:观察不同压力高压氧(hyperbaric oxygen,HBO)对脑出血(intracerebral hemorrhage,ICH)大鼠出血灶周围组织Bcl-2/Bax比值的影响。方法:应用胶原酶诱导法建立大鼠脑出血模型,将90只雄性脑出血SD大鼠随机分为对照组、高压氧治疗组:1.0ATA组、1.8ATA组、2.0ATA组、2.2ATA组,每组18只,按不同治疗压力于造模后24h进行治疗,1次/d,各组分别于造模成功后第1、3、5天断头取脑,每个时间点各6只。免疫组织化学染色法测定Bcl-2与Bax的表达,对Bcl-2/Bax比值进行统计分析。结果:Bcl-2/Bax比值:第1天时,高压氧治疗组较对照组升高,差异有显著性意义(P<0.05),1.8ATA组、2.0ATA组、2.2ATA组较1.0ATA组升高,差异有显著性意义(P<0.05),2.2ATA组、2.0ATA组、1.8ATA组依次升高,1.8ATA组、2.0ATA组与2.2ATA组比较差异有显著性意义(P<0.05),1.8ATA组与2.0ATA组比较差异无显著性意义(P>0.05);第3天时,高压氧治疗组较对照组升高,1.0ATA组与对照组比较差异无显著性意义(P>0.05),1.8ATA组、2.0ATA组、2.2ATA组与对照组比较差异有显著性意义(P<0.05),2.2ATA组、2.0ATA组、1.8ATA组依次升高,各组之间比较差异均有显著性意义(P<0.05);第5天时,高压氧治疗组较对照组升高,差异均有显著性意义(P<0.05),2.0ATA组、2.2ATA组低于1.0ATA组,差异无显著性意义(P>0.05),1.8ATA组高于1.0ATA组、2.0ATA组、2.2ATA组,差异有显著性意义(P<0.05)。结论:HBO治疗可提高出血灶周围脑组织Bcl-2/Bax的比值,抑制出血灶周围组织细胞凋亡,且1.8ATA在提高Bcl-2/Bax的比值上较优。
Objective:To study the effect of different atmosphere absolute(ATA) hyperbaric oxygen(HBO) on the ratio of Bcl-2/Bax in rats with intracerebral hemorrhage(ICH).Method:The collagenase-induced method was used to set up the rat models of ACH.A total of 90 male SD rats with experimental intracerebral hemorrhage were randomly divided as ICH control group, CH with HBO therapy group, 1.0 ATA group, 1.8 ATA group, 2.0 ATA group, 2.2 ATA group,with 18 rats in each group.The oxygen treatment was administered by using 90% oxygen at different ATA, 24 hours after ICH, once daily. Rats were sacrificed by decollation at the time points of 1 d, 3 d, 5 d. Each time point had 6 rats.The expressions of Bcl-2 and Bax were detected by immunohistochemical staining. Statistical analysis for the ratio of Bcl-2/Bax was performed.Result: The ratio of Bcl-2/Bax increased from 1 d to 5 d gradually for the control ICH group. The ATA groups increased gradually also but significantly more than the control group at any time point. The most increasement happened in 1.8 ATA group. The 2.0 and 2.2 ATA groups increased less and less gradually.The least is the 1.0 ATA group.Conclusion:HBO therapy can increase the ratio of Bcl-2/Bax and play a role of inhibition of nerve cell apoptosis and neuroprotection.1.8 ATA was superior to others.
Objective:To study the effect of different atmosphere absolute(ATA) hyperbaric oxygen(HBO) on the ratio of Bcl-2/Bax in rats with intracerebral hemorrhage(ICH).Method:The collagenase-induced method was used to set up the rat models of ACH.A total of 90 male SD rats with experimental intracerebral hemorrhage were randomly divided as ICH control group, CH with HBO therapy group, 1.0 ATA group, 1.8 ATA group, 2.0 ATA group, 2.2 ATA group,with 18 rats in each group.The oxygen treatment was administered by using 90% oxygen at different ATA, 24 hours after ICH, once daily. Rats were sacrificed by decollation at the time points of 1 d, 3 d, 5 d. Each time point had 6 rats.The expressions of Bcl-2 and Bax were detected by immunohistochemical staining. Statistical analysis for the ratio of Bcl-2/Bax was performed.Result: The ratio of Bcl-2/Bax increased from 1 d to 5 d gradually for the control ICH group. The ATA groups increased gradually also but significantly more than the control group at any time point. The most increasement happened in 1.8 ATA group. The 2.0 and 2.2 ATA groups increased less and less gradually.The least is the 1.0 ATA group.Conclusion:HBO therapy can increase the ratio of Bcl-2/Bax and play a role of inhibition of nerve cell apoptosis and neuroprotection.1.8 ATA was superior to others.
引文
[1]赵性泉,王拥军,王春雪,等.大鼠脑出血模型血肿周围继发损害的分子生物学机制研究[J].首都医科大学学报,2005,26(1):77-80.
[2]吴伟,汪洋,邱阳.高压氧对持续局灶性脑缺血细胞凋亡的影响及其作用机制[J].中国物理医学与康复医学杂志,2003,25(8)458-461.
[3]陈静,尤春景.高压氧对脑外伤大鼠海马神经元凋亡的影响[J]中国物理医学与康复医学杂志,2006,28(6):361-363.
[4]Qin Z,Xi G,Keep RF,et al.Hyperbaric oxygen for experimental intra-cerebral hemorrhage[J].Acta Neurochir Suppl,2008105:13-117.
[5]魏秀娥,荣良群.实验性大鼠脑出血脑内Bax、Bcl-2表达的实验研究[J].中国实用神经疾病杂志,2010,13(24):1-3.
[6]李红玲.胶原酶诱导的脑出血大鼠模型及其行为学改变[J].中国康复医学杂志,2006,21(11):977-979.
[7]张磊.采用Ⅶ型胶原酶构建大鼠脑出血模型[J].江汉大学学报(自然科学版),2009,37(2):82-84.
[8]Longa EZ,Weinstein PR,Carlson S,et al.Reversible middle cerebral artery exclusion without craniectomy in rats[J].Stroke1989,20:84-91.
[9]徐春江.不同压力高压氧对脑创伤大鼠顶叶皮质神经元超微结构的影响[J].中国全科医学,2008,11(8A):1358-1360.
[10]Gurol ME,Krespi Y,Tugcu B,et al.Istanbul Stroke Registry analysis of 1260 consecutive patients[J].Eur J Neurol,2001,8(Suppl 2):13-14.
[11]Massaro AR,Sacco RL,Mohr JP,et al.Clinical discriminators of lobar and deep hemorrhages:the Stroke Data Bank[J]Neurology,1991,41:1881-1885.
[12]Flaherty ML,Haverbusch M,Sekar P,et al.Long-term mortality after intracerebral hemorrhage[J].Neurology,2006,66:1182-1186.
[13]詹传伟,郑仲贤.中少量高血压脑出血微创手术治疗的临床疗效观察[J].当代医学,2012,18(17):80-81.
[14]吴岚,齐立,刘开祥,等.大鼠脑出血模型出血灶周围神经细胞凋亡的研究[J].国际医药卫生导报,2007,13(21):9-13.
[15]孙晓雪,刘斌.细胞凋亡与缺血性脑血管病[J].中国煤炭工业医学杂志,2008,11(4):593-595.
[16]吴岚,刘开祥,俸军林,等.三七总皂苷对脑出血大鼠细胞凋亡及Bcl-2和Bax表达的影响[J].中国中医急症,2012,21(10):1592.
[17]王爱岳,李强,周治平,等.高压氧对脑出血大鼠脑内HIF-1α表达和脑水肿的影响[J].海南医学,2016,23(12):15-17.
[18]张征,马晓莉,田艳娟.高压氧治疗高血压脑出血患者的治疗时机探讨[J].宁夏医学杂志,2016,38(8):717-718.
[19]McCourt R,Gould B,Gioia L,et al.Cerebral perfusion and blood pressure do not affect perihematoma edema growth in acute intrcerebral hemorrhage[J].Storke,2014,45:1292-1298.
[20]李萍,赵树明,胡亚男,等.脑出血发病机制研究进展[J].中华老年心脑血管病杂志,2015,17(2):214-215.
[21]Huang ZX,Kang ZM,Gu GJ,et al.Therapeutic effects of hyperbaric oxygen in a rat model of endothelin-1-induced focal cerebral ischemia[J].Brain Res,1153(2007):204-213.
[22]周建光,刘景昌,方以群.高压氧对脑缺血再灌注海马CA1区神经元凋亡作用的研究[J].中国应用生理学杂志,2001,17(1):82-84.
[23]高宇,高春锦,杨晶,等.高压氧综合治疗心肺复苏术后意识障碍患者的疗效观察[J].中华航海医学与高气压医学杂志,2014,21(2):109-111.
[24]Badr AE,Yin W,Mychaskiw G,et al.Effect of hyperbaric oxygen on striatal metabolites:a miero dialysis study in awake freely moving rats after MCA occlusion[J].Brain Res,2001,916(1/2):85-90.
[25]王远传,雷町,冯凌,等.高压氧对鼠脑创伤后基因mdm2、bcl-2、bax表达的影响[J].临床和实验医学杂志,2008,7(8):15-16.
[26]陈玉燕,李红玲,牛蕾蕾,等.不同压力高压氧对实验性脑出血大鼠出血灶周围水肿及水通道蛋白4表达的影响[J].中国康复医学杂志,2013,28(5):413-417.
[2]吴伟,汪洋,邱阳.高压氧对持续局灶性脑缺血细胞凋亡的影响及其作用机制[J].中国物理医学与康复医学杂志,2003,25(8)458-461.
[3]陈静,尤春景.高压氧对脑外伤大鼠海马神经元凋亡的影响[J]中国物理医学与康复医学杂志,2006,28(6):361-363.
[4]Qin Z,Xi G,Keep RF,et al.Hyperbaric oxygen for experimental intra-cerebral hemorrhage[J].Acta Neurochir Suppl,2008105:13-117.
[5]魏秀娥,荣良群.实验性大鼠脑出血脑内Bax、Bcl-2表达的实验研究[J].中国实用神经疾病杂志,2010,13(24):1-3.
[6]李红玲.胶原酶诱导的脑出血大鼠模型及其行为学改变[J].中国康复医学杂志,2006,21(11):977-979.
[7]张磊.采用Ⅶ型胶原酶构建大鼠脑出血模型[J].江汉大学学报(自然科学版),2009,37(2):82-84.
[8]Longa EZ,Weinstein PR,Carlson S,et al.Reversible middle cerebral artery exclusion without craniectomy in rats[J].Stroke1989,20:84-91.
[9]徐春江.不同压力高压氧对脑创伤大鼠顶叶皮质神经元超微结构的影响[J].中国全科医学,2008,11(8A):1358-1360.
[10]Gurol ME,Krespi Y,Tugcu B,et al.Istanbul Stroke Registry analysis of 1260 consecutive patients[J].Eur J Neurol,2001,8(Suppl 2):13-14.
[11]Massaro AR,Sacco RL,Mohr JP,et al.Clinical discriminators of lobar and deep hemorrhages:the Stroke Data Bank[J]Neurology,1991,41:1881-1885.
[12]Flaherty ML,Haverbusch M,Sekar P,et al.Long-term mortality after intracerebral hemorrhage[J].Neurology,2006,66:1182-1186.
[13]詹传伟,郑仲贤.中少量高血压脑出血微创手术治疗的临床疗效观察[J].当代医学,2012,18(17):80-81.
[14]吴岚,齐立,刘开祥,等.大鼠脑出血模型出血灶周围神经细胞凋亡的研究[J].国际医药卫生导报,2007,13(21):9-13.
[15]孙晓雪,刘斌.细胞凋亡与缺血性脑血管病[J].中国煤炭工业医学杂志,2008,11(4):593-595.
[16]吴岚,刘开祥,俸军林,等.三七总皂苷对脑出血大鼠细胞凋亡及Bcl-2和Bax表达的影响[J].中国中医急症,2012,21(10):1592.
[17]王爱岳,李强,周治平,等.高压氧对脑出血大鼠脑内HIF-1α表达和脑水肿的影响[J].海南医学,2016,23(12):15-17.
[18]张征,马晓莉,田艳娟.高压氧治疗高血压脑出血患者的治疗时机探讨[J].宁夏医学杂志,2016,38(8):717-718.
[19]McCourt R,Gould B,Gioia L,et al.Cerebral perfusion and blood pressure do not affect perihematoma edema growth in acute intrcerebral hemorrhage[J].Storke,2014,45:1292-1298.
[20]李萍,赵树明,胡亚男,等.脑出血发病机制研究进展[J].中华老年心脑血管病杂志,2015,17(2):214-215.
[21]Huang ZX,Kang ZM,Gu GJ,et al.Therapeutic effects of hyperbaric oxygen in a rat model of endothelin-1-induced focal cerebral ischemia[J].Brain Res,1153(2007):204-213.
[22]周建光,刘景昌,方以群.高压氧对脑缺血再灌注海马CA1区神经元凋亡作用的研究[J].中国应用生理学杂志,2001,17(1):82-84.
[23]高宇,高春锦,杨晶,等.高压氧综合治疗心肺复苏术后意识障碍患者的疗效观察[J].中华航海医学与高气压医学杂志,2014,21(2):109-111.
[24]Badr AE,Yin W,Mychaskiw G,et al.Effect of hyperbaric oxygen on striatal metabolites:a miero dialysis study in awake freely moving rats after MCA occlusion[J].Brain Res,2001,916(1/2):85-90.
[25]王远传,雷町,冯凌,等.高压氧对鼠脑创伤后基因mdm2、bcl-2、bax表达的影响[J].临床和实验医学杂志,2008,7(8):15-16.
[26]陈玉燕,李红玲,牛蕾蕾,等.不同压力高压氧对实验性脑出血大鼠出血灶周围水肿及水通道蛋白4表达的影响[J].中国康复医学杂志,2013,28(5):413-417.