荧光分析法在血糖检测中的应用
|
摘要
用不同波长的光激发葡萄糖溶液和血清样品,确定血清的最佳激发波长。以相同波长的光激发含不同血糖浓度的血清样品,得到不同血清的荧光发射光谱,通过分析其荧光光谱判断血清中的血糖浓度大小。实验结果表明,检测血清的最佳激发波长为340nm,血清中葡萄糖的发射峰位置为470nm;随着血清中葡萄糖浓度的增加,荧光光谱图中荧光峰处荧光强度总体呈上升趋势,光谱的面积积分强度和导数光谱中一阶导数的最大值也呈上升趋势,荧光峰的半峰宽整体呈现下降趋势。荧光光谱分析法操作简单,灵敏度高,费用相对较低,为血糖检测提供了有力的参考。
With the glucose solution and serum excited by light in different wavelength,the optimum excitation wavelength of serum was determined.After exciting the serum containing different blood glucose levels with the same light wavelength,this paper obtained the fluorescence emission spectrum of different serum,and then estimated the blood glucose level in the method of analyzing its fluorescence spectrum.The experimental results have demonstrated that the optimal excitation wavelength detecting serum is 340 nm and the emission peak of glucose in serum appears at 470 nm.With the increase of glucose levels,the fluorescence intensity in the fluorescence peak shows an upward trend in the fluorescence spectrum.Besides,there is also growth tendency in the area integrals and the maximum value of the first-order derivative in the derivative spectrum,while the full width at half maximum of the fluorescence peak shows a downward trend as a whole.With the advantages of simple operation,high sensitivity and low cost,fluorescence spectrum analysis provides a method for the detection of blood glucose.
With the glucose solution and serum excited by light in different wavelength,the optimum excitation wavelength of serum was determined.After exciting the serum containing different blood glucose levels with the same light wavelength,this paper obtained the fluorescence emission spectrum of different serum,and then estimated the blood glucose level in the method of analyzing its fluorescence spectrum.The experimental results have demonstrated that the optimal excitation wavelength detecting serum is 340 nm and the emission peak of glucose in serum appears at 470 nm.With the increase of glucose levels,the fluorescence intensity in the fluorescence peak shows an upward trend in the fluorescence spectrum.Besides,there is also growth tendency in the area integrals and the maximum value of the first-order derivative in the derivative spectrum,while the full width at half maximum of the fluorescence peak shows a downward trend as a whole.With the advantages of simple operation,high sensitivity and low cost,fluorescence spectrum analysis provides a method for the detection of blood glucose.
引文
[1]侯清涛,李芸,李舍予,等.全球糖尿病疾病负担现状[J].中国糖尿病杂志,2016,24(01):92—96.Hou Zhiqing,Li Yun,Li Sheyu,et al.Current status of global diabetes disease[J].Chinese Journal of Diabetes,2016,24(01):92—96.
[2]潘盼,赵芳雅,张磊,等.2型糖尿病患者肾功能指标对外周血管病变的评估价值[J].中国糖尿病杂志,2014,6(10):712—716.Pan Pan,Zhao Yafang,Zhang Lei,et al.Association between indicators of kidney function and peripheral vascular diseases in type 2diabetic patients[J].Chinese Journal of Diabetes,2014,6(10):712—716.
[3]Ooi H L,Wu L L.Insulin pump therapy in children and adolescents with Type 1Diabetes:improvements in glycemic control and patients'satisfaction—Hospital UKM experience[J].Medical Journal of Malaysia,2011,66(4):308—312.
[4]Danne T,Bolinder J.New insulins and insulin therapy[J].International Journal of Clinical Practice,2012,65(Supplement s170):26—30.
[5]徐珂.空腹血糖、餐后2h血糖、糖化血红蛋白检测在糖尿病诊断中的临床价值分析[J].中国当代医药,2014,21(13):75—77.Xu Ke.Analysis of the clinical value on fasting plasma glucose,2h postprandial plasma glucose and glycosylated hemoglobin in the diagnostic of diabetes mellitus[J].China Modern Medicine,2014,21(13):75—77.
[6]陈杨.基于SOC系统的半自动生化分析仪的设计[D].长春:吉林大学,2014:9—11.Chen Yang.Design of the Semi-automatic biochemical analyzer based on SOC system[D].Changchun:Jilin University,2014:9—11.
[7]杨勇.基于STM32全自动生化分析仪的研究与设计[D].沈阳:沈阳工业大学,2016:12—13.Yang Yong.The research and design of automatic biochemical analyzer based on STM32[D].Shenyang:Shenyang University of Technology,2016:12—13.
[8]栗大超,蒲治华,于海霞,等.微创伤血糖连续检测技术及仪器的研究进展[J].纳米技术与精密工程,2016,14(4):269—277.Li Dachao,Pu Zhihua,Yu Haixia,et al.Research status of minimally invasive continuous blood glucose monitoring technology and instruments[J].Nanotechnology and Precision Engineering,2016,14(4):269—277.
[9]朱卫华,赵志敏,郑敏,等.人体血清血糖浓度对血清荧光强度的影响[J].光谱学与光谱分析,2007,27(12):2531—2533.Zhu Weihua,Zhao Zhimin,Zheng Min,et al.Effect of human serum glucose concentration on serum fluorescence intensity[J].Spectroscopy and Spectral Analysis,2007,27(12):2531—2533.
[10]王乐新,赵志敏,辛玉军,等.不同波长激发光对血清荧光光谱影响的实验研究[J].光谱学与光谱分析,2008,28(10):2360—2364.Wang Lexin,Zhao Zhimin,Xin Yujun,et al.Study of influence spectrum of different wavelength excitation light on serum fluorescence spectrum[J].Spectroscopy and Spectral Analysis,2008,28(10):2360—2364.
[11]Shirshin E,Cherkasova O,Tikhonova T,et al.Native fluorescence spectroscopy of blood plasma of rats with experimental diabetes:identifying fingerprints of glucose-related metabolic pathways[J].Journal of Biomedical Optics,2015,20(5):51033.
[12]Abdallah O,Qananwah Q,Bolz A,et al.Fluorescence spectroscopy by detection of glucose concentrations in DMEM-solutions and its Perspectives for non-invasive measurement[C]∥Biodevices2011-Proceedings of the International Conference on Biomedical Electronics and Devices.Rome,Italy:DBLP,2015:411—414.
[13]李昊,陈明惠.荧光光谱分析法在地沟油鉴别中的应用研究[J].激光生物学报,2014,23(5):472—476.Li Hao,Chen Minghui.Application of fluorescence spectrum analysis in identification of waste 0il[J].Acta Laser Biology Sinica,2014,23(5):472—476.
[14]林上忠,陈荣,许少锋.人血液不同成份吸收光谱探讨[J].中华物理医学与康复杂志,1998,15(1):26—28.Lin Shangzhong,Chen Rong,Xu Shaofeng.Absorption spectrum of blood components in man[J].Chinese Journal of Physical Medicine and Rehabilitation,1998,15(1):26—28.
[15]王乐新,赵志敏,等.血清浓度变化对血清荧光光谱的影响[J].光谱学与光谱分析,2009,29(5):1336—1340.Wang Lexin,Zhao Zhimin,et al.Effect of serum concentration on serum fluorescence spectrum[J].Spectroscopy and Spectral Analysis,2009,29(5):1336—1340.
[16]王畅,王乐新,朱文霞.奶牛血液的荧光特性分析[J].沈阳农业大学学报,2013,44(04):509—511.Wang Chang,Wang Lexin,Zhu Wenxia.Experimental investigation of fluorescence characteristics of cow's blood[J].Journal of Shenyang Agricultural University,2013,44(04):509—511.
[2]潘盼,赵芳雅,张磊,等.2型糖尿病患者肾功能指标对外周血管病变的评估价值[J].中国糖尿病杂志,2014,6(10):712—716.Pan Pan,Zhao Yafang,Zhang Lei,et al.Association between indicators of kidney function and peripheral vascular diseases in type 2diabetic patients[J].Chinese Journal of Diabetes,2014,6(10):712—716.
[3]Ooi H L,Wu L L.Insulin pump therapy in children and adolescents with Type 1Diabetes:improvements in glycemic control and patients'satisfaction—Hospital UKM experience[J].Medical Journal of Malaysia,2011,66(4):308—312.
[4]Danne T,Bolinder J.New insulins and insulin therapy[J].International Journal of Clinical Practice,2012,65(Supplement s170):26—30.
[5]徐珂.空腹血糖、餐后2h血糖、糖化血红蛋白检测在糖尿病诊断中的临床价值分析[J].中国当代医药,2014,21(13):75—77.Xu Ke.Analysis of the clinical value on fasting plasma glucose,2h postprandial plasma glucose and glycosylated hemoglobin in the diagnostic of diabetes mellitus[J].China Modern Medicine,2014,21(13):75—77.
[6]陈杨.基于SOC系统的半自动生化分析仪的设计[D].长春:吉林大学,2014:9—11.Chen Yang.Design of the Semi-automatic biochemical analyzer based on SOC system[D].Changchun:Jilin University,2014:9—11.
[7]杨勇.基于STM32全自动生化分析仪的研究与设计[D].沈阳:沈阳工业大学,2016:12—13.Yang Yong.The research and design of automatic biochemical analyzer based on STM32[D].Shenyang:Shenyang University of Technology,2016:12—13.
[8]栗大超,蒲治华,于海霞,等.微创伤血糖连续检测技术及仪器的研究进展[J].纳米技术与精密工程,2016,14(4):269—277.Li Dachao,Pu Zhihua,Yu Haixia,et al.Research status of minimally invasive continuous blood glucose monitoring technology and instruments[J].Nanotechnology and Precision Engineering,2016,14(4):269—277.
[9]朱卫华,赵志敏,郑敏,等.人体血清血糖浓度对血清荧光强度的影响[J].光谱学与光谱分析,2007,27(12):2531—2533.Zhu Weihua,Zhao Zhimin,Zheng Min,et al.Effect of human serum glucose concentration on serum fluorescence intensity[J].Spectroscopy and Spectral Analysis,2007,27(12):2531—2533.
[10]王乐新,赵志敏,辛玉军,等.不同波长激发光对血清荧光光谱影响的实验研究[J].光谱学与光谱分析,2008,28(10):2360—2364.Wang Lexin,Zhao Zhimin,Xin Yujun,et al.Study of influence spectrum of different wavelength excitation light on serum fluorescence spectrum[J].Spectroscopy and Spectral Analysis,2008,28(10):2360—2364.
[11]Shirshin E,Cherkasova O,Tikhonova T,et al.Native fluorescence spectroscopy of blood plasma of rats with experimental diabetes:identifying fingerprints of glucose-related metabolic pathways[J].Journal of Biomedical Optics,2015,20(5):51033.
[12]Abdallah O,Qananwah Q,Bolz A,et al.Fluorescence spectroscopy by detection of glucose concentrations in DMEM-solutions and its Perspectives for non-invasive measurement[C]∥Biodevices2011-Proceedings of the International Conference on Biomedical Electronics and Devices.Rome,Italy:DBLP,2015:411—414.
[13]李昊,陈明惠.荧光光谱分析法在地沟油鉴别中的应用研究[J].激光生物学报,2014,23(5):472—476.Li Hao,Chen Minghui.Application of fluorescence spectrum analysis in identification of waste 0il[J].Acta Laser Biology Sinica,2014,23(5):472—476.
[14]林上忠,陈荣,许少锋.人血液不同成份吸收光谱探讨[J].中华物理医学与康复杂志,1998,15(1):26—28.Lin Shangzhong,Chen Rong,Xu Shaofeng.Absorption spectrum of blood components in man[J].Chinese Journal of Physical Medicine and Rehabilitation,1998,15(1):26—28.
[15]王乐新,赵志敏,等.血清浓度变化对血清荧光光谱的影响[J].光谱学与光谱分析,2009,29(5):1336—1340.Wang Lexin,Zhao Zhimin,et al.Effect of serum concentration on serum fluorescence spectrum[J].Spectroscopy and Spectral Analysis,2009,29(5):1336—1340.
[16]王畅,王乐新,朱文霞.奶牛血液的荧光特性分析[J].沈阳农业大学学报,2013,44(04):509—511.Wang Chang,Wang Lexin,Zhu Wenxia.Experimental investigation of fluorescence characteristics of cow's blood[J].Journal of Shenyang Agricultural University,2013,44(04):509—511.