紫菜对大鼠生理机能及体内铅含量的影响
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摘要
通过30 d喂养实验研究条斑紫菜和坛紫菜对大鼠体质量、肝肾功能和抗氧化系统的影响,并以铅为例,分析紫菜中的铅在各器官组织中的蓄积情况,为评价紫菜的食用安全性奠定基础。实验结果表明,条斑紫菜和坛紫菜均具有促进大鼠体质量增长的作用;紫菜对大鼠肝脏、肾脏等相关功能指示性指标无显著影响,能够维持机体正常的生理机能;条斑紫菜和坛紫菜能够增强抗氧化防御作用,坛紫菜作用效果显著。条斑紫菜和坛紫菜各剂量组大鼠肝脏、肾脏中铅含量与空白对照无显著性差异,并未在大鼠肝肾中蓄积,当粪便中铅含量高时,对应血液中铅含量较低,条斑紫菜中的铅更易随粪便排出大鼠体外。
The effects of Pyropia yezoensis and P.haitanensis on the body mass,function of liver and kidney,antioxidant system of rats were studied by the thirty days feeding experiment,and the lead content in organs of rats were also determined for providing the basis of evaluating the safety of laver.The results showed that these two kinds of laver could promote the body mass of rats,and had no significant effects on liver and kidney functions,which indicated that laver could maintain the physiological function of rats at a normal level.P.yezoensis and P.haitanensis could increase the antioxidant defense function of rats,which was significantly increased when treating with P.haitanensis.There were no significant differences of lead contents in liver and kidney of rats between the laver group and control group,suggesting lead from laver was not accumulated in liver and kidney.The lead accumulated in blood became less with the lead content in feces increasing,and lead from P.yezoensis was easier to be excreted out of body than that from P.haitanensis.
The effects of Pyropia yezoensis and P.haitanensis on the body mass,function of liver and kidney,antioxidant system of rats were studied by the thirty days feeding experiment,and the lead content in organs of rats were also determined for providing the basis of evaluating the safety of laver.The results showed that these two kinds of laver could promote the body mass of rats,and had no significant effects on liver and kidney functions,which indicated that laver could maintain the physiological function of rats at a normal level.P.yezoensis and P.haitanensis could increase the antioxidant defense function of rats,which was significantly increased when treating with P.haitanensis.There were no significant differences of lead contents in liver and kidney of rats between the laver group and control group,suggesting lead from laver was not accumulated in liver and kidney.The lead accumulated in blood became less with the lead content in feces increasing,and lead from P.yezoensis was easier to be excreted out of body than that from P.haitanensis.
引文
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[3]张全斌,赵婷婷,綦慧敏,等.紫菜的营养价值研究概况[J].海洋科学,2005,29(2):69-72.
[4]姚兴存,王洪斌,舒留泉,等.条斑紫菜与坛紫菜蛋白组成与生物活性比较研究[J].水产科学,2013,32(4):215-218.
[5]王治.紫菜生物活性成分研究进展[J].食品研究与开发,2017,38(10):215-218.
[6]李恺,耿存珍,张阳,等.活性和非活性海藻吸附重金属的研究[J].环境工程,2013,31(1):34,51-55.
[7]谢华林.微波消解电感耦合等离子体发射光谱法同时测定水产品中铅镉铬汞砷硒有害元素的研究[J]食品科学,2002,23(2):108-110.
[8]曾繁杰,蒋丽金,徐秀璋,等.中国坛紫菜和条斑紫菜中的氨基酸组成和营养价值的研究[J].海藻与湖沼,1991,22(6):590-593.
[9]袁本利.药物安全评价中脏器系数的意义及不足[J]中国新药杂志,2003,12(11):960-963.
[10]陈小伍,李利红,朱达坚,等.大鼠肝损伤动物模型的建立[J].中华实验外科杂志,2009,26(12):1723-1725.
[11]董书清.血清肌酐、尿素氮的检测值及其比值与肾脏损害的探讨[J].中国医药指南,2012,9(36):136-137.
[12]DEVASAGAYAM T P,TILAK J C,BOLOOR KK,et al.Free radicals and antioxidants in human health:Current status and future prospects[J].Journal of Association of Physicians of India,2004,52:794-804.
[13]PONCE-CANCHIHUAM魣N J C,P魪REZ-M魪NDEZO,HERN魣NDEZ-MU譙OZ R,et al.Protective effects of Spirulina maxima on hyperlipidemia and oxidative-stress induced by lead acetate in the liver and kidney[J].Lipids in Health and Disease,2010,9(1):116-117.
[14]DEWANJEE S,SAHU R,KARMAKAR S,et al Toxic effects of lead exposure in Wistar rats:Involvement of oxidative stress and the beneficial role of edible jute(Corchorus olitorius)leaves[J].Food and Chemical Toxicology,2013,55(3):78-91.
[15]中华人民共和国国家卫生和计划生育委员会,国家食品药品监督管理总局.食品安全国家标准食品中污染物限量:GB 2762-2017[S].北京:中国标准出版社,2017:2.
[16]张建平,庄峙夏,陈成祥,等.大鼠体内铅镉分布的稳定同位素示踪ICP-MS研究[J].分析测试学报,2008,27(8):835-838.
[17]赵丹宇,郑云雁,李晓瑜.食品添加剂与污染物[M]北京:中国标准出版社,2003:475-481.
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