MRFs家族基因在翘嘴鳜成体不同组织及器官中的表达特征
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摘要
生肌调节因子(MRFs)家族成员包括MRF4、Myf5、Myogenin和MyoD,是肌肉形成的关键控制因素,其作为一种转录因子在肌肉的发育分化过程中发挥重要作用。本研究通过RT-qPCR方法分析MRFs家族基因在翘嘴鳜成体中不同组织及器官的表达情况,阐明其在肌肉组织中的特异性表达。结果显示:MRFs家族基因在成体翘嘴鳜肌肉、心脏、肝脏、脾脏、肾脏、肠道和脑组织及器官中均检测到表达,且在肌肉中的表达量显著高于其他组织及器官中的表达量(p<0.05)。为研究生肌调节因子在翘嘴鳜肌肉发育过程中的作用提供了基础资料。
The myogenic regulatory factors family members, including MRF4, Myf5, Myogenin and MyoD, are the key control factors in myogenesis, which plays an important role as a transcription factor in the processes of muscle development and differentiation. Real-time PCR was used to analyze the expression of MRFs family genes in different tissues and organs of adult mandarin fish in this research, and illustrate the specific expression of MRFs in muscle tissue. The results showed that MRFs family gene was expressed in all tested tissues and organs including muscle, heart, liver, spleen, kidney, intestine and brain of adult mandarin fish, among which the expression in muscle was significantly higher than other tissues and organs(p<0.05). The study could provide the initial value for researching the role of MRFs in muscle development of mandarin fish.
The myogenic regulatory factors family members, including MRF4, Myf5, Myogenin and MyoD, are the key control factors in myogenesis, which plays an important role as a transcription factor in the processes of muscle development and differentiation. Real-time PCR was used to analyze the expression of MRFs family genes in different tissues and organs of adult mandarin fish in this research, and illustrate the specific expression of MRFs in muscle tissue. The results showed that MRFs family gene was expressed in all tested tissues and organs including muscle, heart, liver, spleen, kidney, intestine and brain of adult mandarin fish, among which the expression in muscle was significantly higher than other tissues and organs(p<0.05). The study could provide the initial value for researching the role of MRFs in muscle development of mandarin fish.
引文
Daubas P.,Tajbakhsh S.,Hadchouel J.,Primig M.,and Buckingham M.,2000,Myf5 is a novel early axonal marker in the mouse brain and is subjected to post-transcriptional regulation in neurons,Development,127(2):319-331
Edmondson D.G.,and Olson E.N.,1993,Helix-looP-helix proteins as regulators of muscle-specific transcription,Journal of Biological Chemistry,268(2):755-758
Ekmark M.,Rana Z.A.,Stewart G.,Hardie D.G.,and Gundersen K.,2007,De-phosphorylation of MyoD is linking nerve-evoked activity to fast myosin heavy chain expression in rodent adult skeletal muscle,Journal of Physiology,584(2):637-650
Filvaroff E.H.,and Derynek R.,1996,Induction of myogenesis in mesenchymal cells by MyoD depends on their of differentiation,Developmental Biology,178(2):459-471
Gregory D.J.,Waldbieser G.C.,and Bosworth B.G.,2004,Cloning and characterization of myogenic regulatory genes in three Ictalurid species,Animal Genetics,35(6):425-430
Hughes S.M.,and Schiaffino S.,1999,Control of muscle fiber size:a crucial factor in ageing,Acta.Physiologica.Scandinavica.,167(4):307-312
Johansen K.A.,and Overturf K.,2005,Sequence,conservation and quantitative expression of rainbow trout Myf5,Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,140(4):533-541
Kim H.J.,Güth R.,Jonsson C.B.,and Unguez G.A.,2009,S.macrurus myogenic regulatory factors induce mammalian skeletal muscle differentiation:Evidence for functional conservation of MRFs,International Journal of Developmental Biology,53(7):993-1002
Li H.H.,Xu Y.Q.,Liu X.Y.,Liu Z.X.,Wang K.Z.,Chu W.Y.,Zhang J.S.,and Ding Z.K.,2013,Molecular cloning of myogenic regulatory factor 4 gene(mrf4)and its expression in adultand embryonic mandarin fish(Siniperca chuatsi),Hunan Nongye Daxue Xuebao(Journal of Hunan Agricultural University),39(6):631-635(李虹辉,许友卿,刘小燕,刘知行,王开卓,褚武英,张建社,丁兆坤,2013,鳜鱼生肌调节因子(mrf4)的克隆及其在成鱼和胚胎中的表达,湖南农业大学学报,39(6):631-635)
Maak S.,Neumann K.,and Swalve H.H.,2006,Identification and analysis of putative regulatory sequences for the MYF5/MYF6 locus in different vertebrate species,Gene,379(9):141-147
Pas M.F.,Harders F.L.,Soumillion A.,Born L.,Buist W.,and Meuwissen T.H.E.,1999,Genetic variation at the porcine Myf5 gene locus,Lack of association with meat production traits,Mammalian Genome,10(2):123-127
Ren H.,Li J.,Li J.T.,Liang Z.X.,Liang J.P.,Ge Q.Q.,and Liu P.,2014,Effects of acute ammonia stresses on antioxidant enzyme activities and GPx gene expression in Exopalaemon carinicauda,Nongye Huanjing Kexue Xuebao(Journal of A-gro-Environment Science),33(4):647-655(任海,李健,李吉涛,梁忠秀,梁俊平,葛倩倩,刘萍,2014,急性氨氮胁迫对脊尾白虾(Exopalaemon carinicauda)抗氧化系统酶活力及GPx基因表达的影响,农业环境科学学报,33(4):647-655)
Sang Q.,2010,Molecular cloning and expression patterns of four MRFs genes in orange-spotted grouper,Epinephelus coioides,Thesis for M.S.,Sun Yat-sen University,Supervisor:Lin H.R.,pp.1-72(桑庆,2010,斜带石斑鱼四种生肌调节因子基因的cDNA克隆及其表达模式分析,硕士学位论文,中山大学,导师:林浩然,pp.1-72)
Tan X.G.,and Du S.J.,2002,Differential expression of two MyoD genes in fast and slow muscles of gilthead seabream(Sparus aurata),Development Genes and Evolution,212(5):207-217
Tan X.G.,Zhang Y.Q.,Zhang P.J.,Xu P.,and Xu Y.L.,2006,Molecular structure and expression patterns of flounder(Paralichthys olivaceus)Myf-5,a myogenic regulatory factor,Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,145(2):204-213
Wang D.L.,and Mao B.L.,1995,The effect and mechanism of myogenic regulatory factors,Yixue Fenzi Shengwuxue Zazhi(Journal of Medical Molecular Biology),17(4):157-160(王东林,毛宝龄,1995,生肌调节因子及其作用机理,医学分子生物学杂志,17(4):157-160)
Wu C.Y.,Wu Z.C.,and Song H.X.,2011,Effects of exercise-induced fatigue on the GDNF and GFRα-1 m RNA expression in hippocampus of mice,Zhongguo Yundong Yixue Zazhi(Chinese Journal of Sports Medicine),30(1):53-56(吴春燕,吴智春,宋惠霄,2011,运动疲劳对小鼠海马GDNF及其受体GFRα-1 m RNA表达的影响,中国运动医学杂志,30(1):53-56)
Zhang Y.Q.,Tan X.G.,Zhang P.J.,and Xu Y.L.,2006,Characterization of muscle-regulatory gene,MyoD,from flounder(Paralichthys olivaceus)and analysis of its expression patterns during embryogenesis,Marine Biotechnology,8(2):139-148
Edmondson D.G.,and Olson E.N.,1993,Helix-looP-helix proteins as regulators of muscle-specific transcription,Journal of Biological Chemistry,268(2):755-758
Ekmark M.,Rana Z.A.,Stewart G.,Hardie D.G.,and Gundersen K.,2007,De-phosphorylation of MyoD is linking nerve-evoked activity to fast myosin heavy chain expression in rodent adult skeletal muscle,Journal of Physiology,584(2):637-650
Filvaroff E.H.,and Derynek R.,1996,Induction of myogenesis in mesenchymal cells by MyoD depends on their of differentiation,Developmental Biology,178(2):459-471
Gregory D.J.,Waldbieser G.C.,and Bosworth B.G.,2004,Cloning and characterization of myogenic regulatory genes in three Ictalurid species,Animal Genetics,35(6):425-430
Hughes S.M.,and Schiaffino S.,1999,Control of muscle fiber size:a crucial factor in ageing,Acta.Physiologica.Scandinavica.,167(4):307-312
Johansen K.A.,and Overturf K.,2005,Sequence,conservation and quantitative expression of rainbow trout Myf5,Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,140(4):533-541
Kim H.J.,Güth R.,Jonsson C.B.,and Unguez G.A.,2009,S.macrurus myogenic regulatory factors induce mammalian skeletal muscle differentiation:Evidence for functional conservation of MRFs,International Journal of Developmental Biology,53(7):993-1002
Li H.H.,Xu Y.Q.,Liu X.Y.,Liu Z.X.,Wang K.Z.,Chu W.Y.,Zhang J.S.,and Ding Z.K.,2013,Molecular cloning of myogenic regulatory factor 4 gene(mrf4)and its expression in adultand embryonic mandarin fish(Siniperca chuatsi),Hunan Nongye Daxue Xuebao(Journal of Hunan Agricultural University),39(6):631-635(李虹辉,许友卿,刘小燕,刘知行,王开卓,褚武英,张建社,丁兆坤,2013,鳜鱼生肌调节因子(mrf4)的克隆及其在成鱼和胚胎中的表达,湖南农业大学学报,39(6):631-635)
Maak S.,Neumann K.,and Swalve H.H.,2006,Identification and analysis of putative regulatory sequences for the MYF5/MYF6 locus in different vertebrate species,Gene,379(9):141-147
Pas M.F.,Harders F.L.,Soumillion A.,Born L.,Buist W.,and Meuwissen T.H.E.,1999,Genetic variation at the porcine Myf5 gene locus,Lack of association with meat production traits,Mammalian Genome,10(2):123-127
Ren H.,Li J.,Li J.T.,Liang Z.X.,Liang J.P.,Ge Q.Q.,and Liu P.,2014,Effects of acute ammonia stresses on antioxidant enzyme activities and GPx gene expression in Exopalaemon carinicauda,Nongye Huanjing Kexue Xuebao(Journal of A-gro-Environment Science),33(4):647-655(任海,李健,李吉涛,梁忠秀,梁俊平,葛倩倩,刘萍,2014,急性氨氮胁迫对脊尾白虾(Exopalaemon carinicauda)抗氧化系统酶活力及GPx基因表达的影响,农业环境科学学报,33(4):647-655)
Sang Q.,2010,Molecular cloning and expression patterns of four MRFs genes in orange-spotted grouper,Epinephelus coioides,Thesis for M.S.,Sun Yat-sen University,Supervisor:Lin H.R.,pp.1-72(桑庆,2010,斜带石斑鱼四种生肌调节因子基因的cDNA克隆及其表达模式分析,硕士学位论文,中山大学,导师:林浩然,pp.1-72)
Tan X.G.,and Du S.J.,2002,Differential expression of two MyoD genes in fast and slow muscles of gilthead seabream(Sparus aurata),Development Genes and Evolution,212(5):207-217
Tan X.G.,Zhang Y.Q.,Zhang P.J.,Xu P.,and Xu Y.L.,2006,Molecular structure and expression patterns of flounder(Paralichthys olivaceus)Myf-5,a myogenic regulatory factor,Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,145(2):204-213
Wang D.L.,and Mao B.L.,1995,The effect and mechanism of myogenic regulatory factors,Yixue Fenzi Shengwuxue Zazhi(Journal of Medical Molecular Biology),17(4):157-160(王东林,毛宝龄,1995,生肌调节因子及其作用机理,医学分子生物学杂志,17(4):157-160)
Wu C.Y.,Wu Z.C.,and Song H.X.,2011,Effects of exercise-induced fatigue on the GDNF and GFRα-1 m RNA expression in hippocampus of mice,Zhongguo Yundong Yixue Zazhi(Chinese Journal of Sports Medicine),30(1):53-56(吴春燕,吴智春,宋惠霄,2011,运动疲劳对小鼠海马GDNF及其受体GFRα-1 m RNA表达的影响,中国运动医学杂志,30(1):53-56)
Zhang Y.Q.,Tan X.G.,Zhang P.J.,and Xu Y.L.,2006,Characterization of muscle-regulatory gene,MyoD,from flounder(Paralichthys olivaceus)and analysis of its expression patterns during embryogenesis,Marine Biotechnology,8(2):139-148