柑橘渣中柚皮苷的提取纯化工艺
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摘要
研究柑橘渣中柚皮苷的提取纯化工艺,为增加柚皮苷产量提供参考方法,以期大量应用于饲料生产中。采用乙醇提取和超声波辅助提取柑橘渣中的柚皮苷,通过单因素试验和正交试验得到两种方法的最优工艺参数。通过吸附试验,获取最适宜纯化柚皮苷的大孔树脂及最优工艺参数。乙醇提取法的最优工艺参数为:提取时间3.5 h、料液比145 g/ml、乙醇体积分数65%、提取温度80℃,在此条件下,柚皮苷提取率为15.09 mg/g。超声波辅助提取法的最优工艺参数为:乙醇体积分数70%、料液比160 g/ml、提取时间90 min、提取温度80℃、超声波频率500 W,在此条件下,柚皮苷提取率为24.15 mg/g。HPD600具有良好的吸附和解吸效果,HPD600的最优工艺条件为:吸附时间4.0 h、样品pH值4、上样流速2 BV/h、洗脱液浓度0.05 mol/l、解吸流速2 BV/h。乙醇提取和超声波辅助提取成本低,无毒害,易操作,适用于工业生产。HPD600是最适合用于柚皮苷分离纯化的大孔树脂。乙醇或超声波辅助提取,结合大孔树脂纯化工艺,可以提高柑橘渣中柚皮苷的提取效率。
To study the extraction and purification of Naringin in citrus pulp. Using ethanol extraction and ultrasonic extraction to extract Naringin from citrus pulp. The optimal process parameters of the above two methods were obtained by single factor experiments and orthogonal experiments. Through the static and dynamic adsorption tests, the most suitable macroporous resin for the purification of Naringin and the optimum process parameters were obtained. The optimal process parameters of ethanol extraction were as follows: the extraction time was 3.5 h, the solid-liqid ratio was 145 g/ml, the ethanol concentration was 65%, the extraction temperature was 80 ℃. The extraction content of Naringin was 15.09 mg/g by the above method. The optimal process parameters of ultrasonic extraction were as follows: the ethanol concentration was 70%, the solid-liqid ratio was 160 g/ml, the extraction time was 90 min, the extraction temperature was 80 ℃, the ultrasonic power was 500 W. The extraction content of naringin was 24.15 mg/g by the above method. The optimal process conditions of HPD600 were as follows: adsorption time was 4.0 h, sample pH was 4, loading flow rate was 2 BV/h, eluent concentration was 0.05 mol/l, desorption flow rate was 2 BV/h. Ethanol extraction and ultrasonic extraction were low cost, low toxcity and easy operation that could be used for industrial production. HPD600 was the most suitable macroporous resin for the separation and purification of Naringin.
To study the extraction and purification of Naringin in citrus pulp. Using ethanol extraction and ultrasonic extraction to extract Naringin from citrus pulp. The optimal process parameters of the above two methods were obtained by single factor experiments and orthogonal experiments. Through the static and dynamic adsorption tests, the most suitable macroporous resin for the purification of Naringin and the optimum process parameters were obtained. The optimal process parameters of ethanol extraction were as follows: the extraction time was 3.5 h, the solid-liqid ratio was 145 g/ml, the ethanol concentration was 65%, the extraction temperature was 80 ℃. The extraction content of Naringin was 15.09 mg/g by the above method. The optimal process parameters of ultrasonic extraction were as follows: the ethanol concentration was 70%, the solid-liqid ratio was 160 g/ml, the extraction time was 90 min, the extraction temperature was 80 ℃, the ultrasonic power was 500 W. The extraction content of naringin was 24.15 mg/g by the above method. The optimal process conditions of HPD600 were as follows: adsorption time was 4.0 h, sample pH was 4, loading flow rate was 2 BV/h, eluent concentration was 0.05 mol/l, desorption flow rate was 2 BV/h. Ethanol extraction and ultrasonic extraction were low cost, low toxcity and easy operation that could be used for industrial production. HPD600 was the most suitable macroporous resin for the separation and purification of Naringin.
引文
[1]陈锦杭,区硕俊,杨展鹏,等.柑橘皮柚皮苷提取工艺研究[J].应用化工,2016,45(3):508-510.
[2]Oluwafeyisetan A,Olubunmi A,Peter O.Naringin Ameliorates HIV-1 Nucleoside Reverse Transcriptase Inhibitors-Induced Mitochondrial Toxicity[J].Current Hiv Research,2016,14(6):506-516.
[3]吴铿,游琼,黄瑞娜,等.柚皮苷对实验性2型糖尿病心肌病大鼠模型糖脂代谢的影响[J].广东医学,2012,33(13):1863-1866.
[4]Chtourou Y,Aouey B,Aroui S,et al.Anti-apoptotic and anti-inflammatory effects of naringin on cisplatin-induced renal injury in the rat[J].Chemico-Biological Interactions,2016,243:1-9.
[5]Raha S,Yumnam S,Hong G E,et al.Naringin induces autophagy-mediated growth inhibition by downregulating the PI3K/Akt/mTOR cascade via activation of MAPK pathways in AGS cancer cells[J].International Journal of Oncology,2015,47(3):1061-1069.
[6]吴泳江,王敬,王琪,等.柚皮苷的生物学功能及在畜禽生产中的应用[J].中国畜牧杂志,2017,53(7):9-12.
[7]Alhidary I A,Abdelrahman M M.Effect of naringin supplementation on performance and physiological responses of heat stressed lambs[J].Diabetes Care,2014,31(8):1541-1545.
[8]于宏伟,谷维娜,李娜,等.柚皮苷的提取方法及其应用研究进展[J].湖北农业科学,2011,50(8):1516-1518.
[9]耿敬章,周芳.香圆枳壳柚皮苷的提取及其对亚硝酸盐的清除作用[J].食品工业科技,2018(4):44-48.
[10]陈源,余亚白,王琦,等.葡萄柚柚皮苷微波提取工艺[J].山地农业生物学报,2012,31(1):28-32.
[11]井丽丽,吴志莲,董艳琳,等.山楂汁树脂柱层析过程中主要有机酸变化规律探讨[J].离子交换与吸附,2017(1):56-64.
[12]刘彬,曹栋,孟庆然.超滤技术结合大孔吸附树脂纯化低聚果糖[J].食品与机械,2016(2):133-138.
[13]陶波,方梅,张嘉男,等.沙冬青种子总黄酮测定方法及纯化工艺研究[J].生物技术通报,2017,33(5):63-70.
[14]汤冬梅.香柚中类柠檬苦素和柚皮苷提取纯化及二氢查尔酮合成[D].长沙:中南大学,2011.
[15]许鹭.柚皮中柚皮苷的提取、分离纯化及其固体分散体与卵磷脂复合物的制备及表征[D].广州:华南理工大学,2015.
[16]闻永举,申秀丽,易艳萍.柚皮中柚皮苷提取工艺研究[J].江苏农业科学,2008(4):257-258.
[17]李秀娟,周志钦.胡柚皮中柚皮苷的超声提取工艺研究[J].中国食品添加剂,2011(2):142-146.
[18]Michael G N K C M.The Influence of Naringin or Hesperidin Dietary Supplementation on Broiler Meat Quality and Oxidative Stability[J].Plos One,2015,10(10):141652-141656.
[19]李赤翎,俞建,曾亮,等.柚皮苷对猪肉的抗氧化作用的研究[J].食品研究与开发,2008,29(10):20-23.
[2]Oluwafeyisetan A,Olubunmi A,Peter O.Naringin Ameliorates HIV-1 Nucleoside Reverse Transcriptase Inhibitors-Induced Mitochondrial Toxicity[J].Current Hiv Research,2016,14(6):506-516.
[3]吴铿,游琼,黄瑞娜,等.柚皮苷对实验性2型糖尿病心肌病大鼠模型糖脂代谢的影响[J].广东医学,2012,33(13):1863-1866.
[4]Chtourou Y,Aouey B,Aroui S,et al.Anti-apoptotic and anti-inflammatory effects of naringin on cisplatin-induced renal injury in the rat[J].Chemico-Biological Interactions,2016,243:1-9.
[5]Raha S,Yumnam S,Hong G E,et al.Naringin induces autophagy-mediated growth inhibition by downregulating the PI3K/Akt/mTOR cascade via activation of MAPK pathways in AGS cancer cells[J].International Journal of Oncology,2015,47(3):1061-1069.
[6]吴泳江,王敬,王琪,等.柚皮苷的生物学功能及在畜禽生产中的应用[J].中国畜牧杂志,2017,53(7):9-12.
[7]Alhidary I A,Abdelrahman M M.Effect of naringin supplementation on performance and physiological responses of heat stressed lambs[J].Diabetes Care,2014,31(8):1541-1545.
[8]于宏伟,谷维娜,李娜,等.柚皮苷的提取方法及其应用研究进展[J].湖北农业科学,2011,50(8):1516-1518.
[9]耿敬章,周芳.香圆枳壳柚皮苷的提取及其对亚硝酸盐的清除作用[J].食品工业科技,2018(4):44-48.
[10]陈源,余亚白,王琦,等.葡萄柚柚皮苷微波提取工艺[J].山地农业生物学报,2012,31(1):28-32.
[11]井丽丽,吴志莲,董艳琳,等.山楂汁树脂柱层析过程中主要有机酸变化规律探讨[J].离子交换与吸附,2017(1):56-64.
[12]刘彬,曹栋,孟庆然.超滤技术结合大孔吸附树脂纯化低聚果糖[J].食品与机械,2016(2):133-138.
[13]陶波,方梅,张嘉男,等.沙冬青种子总黄酮测定方法及纯化工艺研究[J].生物技术通报,2017,33(5):63-70.
[14]汤冬梅.香柚中类柠檬苦素和柚皮苷提取纯化及二氢查尔酮合成[D].长沙:中南大学,2011.
[15]许鹭.柚皮中柚皮苷的提取、分离纯化及其固体分散体与卵磷脂复合物的制备及表征[D].广州:华南理工大学,2015.
[16]闻永举,申秀丽,易艳萍.柚皮中柚皮苷提取工艺研究[J].江苏农业科学,2008(4):257-258.
[17]李秀娟,周志钦.胡柚皮中柚皮苷的超声提取工艺研究[J].中国食品添加剂,2011(2):142-146.
[18]Michael G N K C M.The Influence of Naringin or Hesperidin Dietary Supplementation on Broiler Meat Quality and Oxidative Stability[J].Plos One,2015,10(10):141652-141656.
[19]李赤翎,俞建,曾亮,等.柚皮苷对猪肉的抗氧化作用的研究[J].食品研究与开发,2008,29(10):20-23.