滋阴养血安神方对PCPA诱导失眠小鼠睡眠的影响
|
摘要
目的:探讨滋阴养血安神方对睡眠剥夺小鼠睡眠的影响及其机制。方法:利用腹腔注射对氯苯丙氨酸(PCPA)制造睡眠剥夺模型,模定失眠小鼠戊巴比妥钠致睡眠的阈下及阈上剂量,分6组,空白对照组、模型组、滋阴养血安神方低剂量组(73 g/kg)、滋阴养血安神方中剂量组(146 g/kg)、滋阴养血安神方高剂量组(292 g/kg)、地西泮组(1.5 mg/kg),造模并给药7 d后,给予戊巴比妥钠诱导睡眠,观察对小鼠睡眠的影响,并检测小鼠海马5-羟色胺(5-HT)含量。结果:滋阴养血安神方可增加阈下剂量戊巴比妥钠小鼠睡眠个数,缩短阈上剂量戊巴比妥钠的小鼠入睡潜伏期,与模型组对比有显著性差异(P<0.01),能延长阈上剂量戊巴比妥钠诱导的睡眠时间,与模型组对比有显著性差异(P<0.01);滋阴养血安神方高剂量组小鼠脑内5-HT含量显著高于模型组,与模型组对比有显著性差异(P<0.05)。结论:滋阴养血安神方具有镇静、催眠作用,对失眠症的有一定的治疗作用,其对小鼠睡眠的影响机制之一可能是通过升高脑内海马5-HT含量实现的。
Objective:To explore the effect of ZiyinYangxueAnshen Decoction on sleep deprivation in mice and its mechanism.Method:The model was established by receiving PCPA, the subthreshold and upper threshold dose of pentobarbital sodium induced sleep in the model insomnia mice were decided.Then six groups such as blank control group, model group, the low dose group of ZiyinYangxueAnshen Decoction(73 g/kg),the dose group of ZiyinYangxueAnshen Decoction(146 g/kg), the high dose group of ZiyinYangxueAnshen Decoction(292 g/kg)and diazepamgroup(1.5 mg/kg) were established. After each drug administration group was given 7 days, pentobarbital sodium was given to induce sleep. Then we observed the sleeping function effects on sleep deprivation mice.The contents of 5-hydroxytryptamine(5-HT) in mice's hippocampus were detected. Result:ZiyinYangxueAnshen Decoction can increase the number of subthreshold dose of sodium pentobarbital mice sleep and shorten the threshold dose of pentobarbital sodium on mice sleep latency, which had significant difference from the model group(P<0.01).ZiyinYangxueAnshen Decoction can also prolong the sleeping timeinduced by pentobarbital sodium compare with the model group(P<0.01).The content of 5-HT detected by ELISA in the high dose of ZiyinYangxueAnshen Decoction was significantly higher than that of model group(P<0.05).Conclusion:ZiyinYangxueAnshen Decoction has sedative and hypnotic effects, and has certain therapeutic effects on insomnia.One of the possible mechanisms that may influence sleep in mice is by increasing the level of 5-HT in the hippocampus.
Objective:To explore the effect of ZiyinYangxueAnshen Decoction on sleep deprivation in mice and its mechanism.Method:The model was established by receiving PCPA, the subthreshold and upper threshold dose of pentobarbital sodium induced sleep in the model insomnia mice were decided.Then six groups such as blank control group, model group, the low dose group of ZiyinYangxueAnshen Decoction(73 g/kg),the dose group of ZiyinYangxueAnshen Decoction(146 g/kg), the high dose group of ZiyinYangxueAnshen Decoction(292 g/kg)and diazepamgroup(1.5 mg/kg) were established. After each drug administration group was given 7 days, pentobarbital sodium was given to induce sleep. Then we observed the sleeping function effects on sleep deprivation mice.The contents of 5-hydroxytryptamine(5-HT) in mice's hippocampus were detected. Result:ZiyinYangxueAnshen Decoction can increase the number of subthreshold dose of sodium pentobarbital mice sleep and shorten the threshold dose of pentobarbital sodium on mice sleep latency, which had significant difference from the model group(P<0.01).ZiyinYangxueAnshen Decoction can also prolong the sleeping timeinduced by pentobarbital sodium compare with the model group(P<0.01).The content of 5-HT detected by ELISA in the high dose of ZiyinYangxueAnshen Decoction was significantly higher than that of model group(P<0.05).Conclusion:ZiyinYangxueAnshen Decoction has sedative and hypnotic effects, and has certain therapeutic effects on insomnia.One of the possible mechanisms that may influence sleep in mice is by increasing the level of 5-HT in the hippocampus.
引文
[1] 张景岳.景岳全书[M].北京:科学技术出版社,1998:59.
[2] 肖成荣,马增春,李海静,等.PCPA失眠大鼠模型的制作及其机制[J].毒理学杂志,2007,21(4):326-326.
[3] 魏伟,吴希美,李元建.药理实验方法学[M].4版.北京:人民卫生出版社,2010:639-640.
[4] Tu Y,Cheng SX,Sun HT. Ferulic acid potentiates pentobarbital-induced sleep via the serotonergic system [J].Neuroscience Letters,2012,525:95-99.
[5] Zhao X,Cui XY,Chen BQ.Tetrandrine,a bisbenzylisoquinoline alkaloid from Chinese herb Radix,augmented the hypnotic effect of pentobarbital through serotonergic system[J]. European Journal of Pharmacology,2004,506:101-105.
[6] Wang LE,Bai YJ,Shi XR. Spinosin,a C-glycoside flavonoid from semen Zizhiphi Spinozae,potentiated pentobarbital-induced sleep via the serotonergic system[J]. Pharmacology Biochemistry and Behavior,2008,90:399-403.
[7] Zhang FY, Li JJ, Zhou Y, et al.Review for sedative and hypnotic mechanism of sedative traditional Chinese medicine and relative active components on neurotransmitters[J].Zhongguo Zhong Yao ZaZhi, 2016,41(23):4320-4327.
[8] 肖成荣,马增春,李海静,等. PCPA失眠大鼠模型的制作及其机制[J]. 毒理学杂志,2007,21(4): 326.
[9] Fidalgo S, Ivanov DK, Wood SH.Serotonin: from top to bottom[J].Biogerontology, 2013,14(1):21-45.
[10] Jo K, Choi HS, Jeon S, et al.Nelumbo nucifera Seed Extract Promotes Sleep in Drosophila melanogaster[J]. Biol Pharm Bull, 2018,41(3):399-408.
[11] Tu Y, Cheng SX, Sun HT, et al.Ferulic acid potentiates pentobarbital-induced sleep via the serotonergic system [J].Neurosci Lett, 2012,525(2):95-99.
[12] Yaxin Zheng, Jiming Wu, Xuesong Feng, et al.In silico Analysis and Experimental Validation of Lignan Extracts from Kadsura longipedunculata for Potential 5-HT1AR Agonists[J]. PLoS One, 2015, 10(6): e0130055.
[13] Huang JC, Xie W, Deng N, et al.Foshouningshen decoction improves sleeping via the serotonergic system in a rat model of insomnia[J].NanFang YiKe DaXue XueBao, 2017,37(8):1116-1120.
[14] Rodriquez Villanueva J, Rodriquez Villanueva L. Experimental and Clinical Pharmacology of Ziziphus jujuba Mills[J].Phytother Res,2017,31(3):347-365.
[15] Wang C, You ZL, Xia Q, et al. Upregulation of Mark3 and Rpgrip1 mRNA expression by jujuboside A in mouse hippocampus[J]. ActaPharmacol Sin,2007,28(3):334-338.
[16] Zhu H, Zhang L, Wang G, et al.Sedative and hypnotic effects of supercritical carbon dioxide fluid extraction from Schisandra chinensis in mice[J].J Food Drug Anal,2016,24(4):831-838.
[17] 宋日新,姜国红,常宝忠,等.参芪五味子颗粒治疗慢性失眠症的效果及安全性观察[J].中国综合临床, 2015,31(10): 931-933.
[18] 肖军,朱易凡,张宁新,等.刺五加注射液对大鼠睡眠影响的实验研究[J]. 河北医药,2009,31(9): 1031-1032
[19] 张淑萍,王亚军,胡春荣,等.刺五加提取物对睡眠剥夺小鼠抗氧化抗疲劳的研究[J].黑龙江医药科学,2015,6:1-3.
[20] 竺工.营养丰富说龙眼补虚助眠有奇效[J]. 长寿,2014,12:32.
[21] Ko CH, Koon CM, Yu SL, et al.Hypnotic effects of a novel anti-insomnia formula on Drosophila insomnia model[J].Chin J Integr Med, 2016, 22(5):335-343.
[22] 失眠多梦吃茯苓[J].中国食用菌,2015,3:27.
[23] 张峥. 形似“小草”,失眠健忘的特效草药—远志[J]. 医药食疗保健,2012,9:27.
[2] 肖成荣,马增春,李海静,等.PCPA失眠大鼠模型的制作及其机制[J].毒理学杂志,2007,21(4):326-326.
[3] 魏伟,吴希美,李元建.药理实验方法学[M].4版.北京:人民卫生出版社,2010:639-640.
[4] Tu Y,Cheng SX,Sun HT. Ferulic acid potentiates pentobarbital-induced sleep via the serotonergic system [J].Neuroscience Letters,2012,525:95-99.
[5] Zhao X,Cui XY,Chen BQ.Tetrandrine,a bisbenzylisoquinoline alkaloid from Chinese herb Radix,augmented the hypnotic effect of pentobarbital through serotonergic system[J]. European Journal of Pharmacology,2004,506:101-105.
[6] Wang LE,Bai YJ,Shi XR. Spinosin,a C-glycoside flavonoid from semen Zizhiphi Spinozae,potentiated pentobarbital-induced sleep via the serotonergic system[J]. Pharmacology Biochemistry and Behavior,2008,90:399-403.
[7] Zhang FY, Li JJ, Zhou Y, et al.Review for sedative and hypnotic mechanism of sedative traditional Chinese medicine and relative active components on neurotransmitters[J].Zhongguo Zhong Yao ZaZhi, 2016,41(23):4320-4327.
[8] 肖成荣,马增春,李海静,等. PCPA失眠大鼠模型的制作及其机制[J]. 毒理学杂志,2007,21(4): 326.
[9] Fidalgo S, Ivanov DK, Wood SH.Serotonin: from top to bottom[J].Biogerontology, 2013,14(1):21-45.
[10] Jo K, Choi HS, Jeon S, et al.Nelumbo nucifera Seed Extract Promotes Sleep in Drosophila melanogaster[J]. Biol Pharm Bull, 2018,41(3):399-408.
[11] Tu Y, Cheng SX, Sun HT, et al.Ferulic acid potentiates pentobarbital-induced sleep via the serotonergic system [J].Neurosci Lett, 2012,525(2):95-99.
[12] Yaxin Zheng, Jiming Wu, Xuesong Feng, et al.In silico Analysis and Experimental Validation of Lignan Extracts from Kadsura longipedunculata for Potential 5-HT1AR Agonists[J]. PLoS One, 2015, 10(6): e0130055.
[13] Huang JC, Xie W, Deng N, et al.Foshouningshen decoction improves sleeping via the serotonergic system in a rat model of insomnia[J].NanFang YiKe DaXue XueBao, 2017,37(8):1116-1120.
[14] Rodriquez Villanueva J, Rodriquez Villanueva L. Experimental and Clinical Pharmacology of Ziziphus jujuba Mills[J].Phytother Res,2017,31(3):347-365.
[15] Wang C, You ZL, Xia Q, et al. Upregulation of Mark3 and Rpgrip1 mRNA expression by jujuboside A in mouse hippocampus[J]. ActaPharmacol Sin,2007,28(3):334-338.
[16] Zhu H, Zhang L, Wang G, et al.Sedative and hypnotic effects of supercritical carbon dioxide fluid extraction from Schisandra chinensis in mice[J].J Food Drug Anal,2016,24(4):831-838.
[17] 宋日新,姜国红,常宝忠,等.参芪五味子颗粒治疗慢性失眠症的效果及安全性观察[J].中国综合临床, 2015,31(10): 931-933.
[18] 肖军,朱易凡,张宁新,等.刺五加注射液对大鼠睡眠影响的实验研究[J]. 河北医药,2009,31(9): 1031-1032
[19] 张淑萍,王亚军,胡春荣,等.刺五加提取物对睡眠剥夺小鼠抗氧化抗疲劳的研究[J].黑龙江医药科学,2015,6:1-3.
[20] 竺工.营养丰富说龙眼补虚助眠有奇效[J]. 长寿,2014,12:32.
[21] Ko CH, Koon CM, Yu SL, et al.Hypnotic effects of a novel anti-insomnia formula on Drosophila insomnia model[J].Chin J Integr Med, 2016, 22(5):335-343.
[22] 失眠多梦吃茯苓[J].中国食用菌,2015,3:27.
[23] 张峥. 形似“小草”,失眠健忘的特效草药—远志[J]. 医药食疗保健,2012,9:27.