黄芩泻火解毒功效关联的质量标志物研究
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摘要
研究不同产地黄芩商品药材中,基于泻火解毒功效关联的黄酮类质量标志物的变化规律,为质量标志物研究提供科学数据.选取黄芩质量标志物组,黄芩素-黄芩苷-汉黄芩素-汉黄芩苷为分析目标物,采用单味药、药对、复方及其相应处理方法制备对照品,用HPLC的方法测定,用数理统计学方法处理数据.结果表明,采用相同样品处理方法,不同产地的黄芩商品药材,其泻火解毒功效相关的黄酮类质量标志物组,具有极显著性差异(P<0.01).不同产地黄芩商品的质量标志物有差异,以河北黄芩黄连解毒汤的质量标志物总百分含量100%计,山西黄芩为66.85%,甘肃黄芩为55.13%,内蒙黄芩为31.16%,建议黄芩药材质量控制加入产地信息.
The change regulation of flavonoid Q-marker which related to purging fire for removing toxin efficacy from different locality of growth was studied to provide scientific data for the study of quality markers. The corresponding method was used to process the samples of the single drug,couplet medicines and compound decoction. The Q-marker group of baicalin,wogonoside,baicalein and scutellarin were estimated by HPLC,and the data were processed with mathematical statistics. Results showed that using the same sample processing method,there was a significant difference( P < 0. 01) in the flavonoid Q-markers in scutellaria baicalensis from different locality of growth. The flavonoid Q-markers in scutellaria baicalensis from different locality of growth were different. Suppose the total Q-marker amount of flavonoids of Hebei scutellaria baicalensis Huanglian jiedu tang was 100%,and the Shanxi scutellaria baicalensis was 66. 85%,Gansu scutellaria baicalensis was 55. 13%,Neimeng scutellaria baicalensis was 31. 16%,it suggested that origin information should add to scutellaria baicalensis quality control system.
The change regulation of flavonoid Q-marker which related to purging fire for removing toxin efficacy from different locality of growth was studied to provide scientific data for the study of quality markers. The corresponding method was used to process the samples of the single drug,couplet medicines and compound decoction. The Q-marker group of baicalin,wogonoside,baicalein and scutellarin were estimated by HPLC,and the data were processed with mathematical statistics. Results showed that using the same sample processing method,there was a significant difference( P < 0. 01) in the flavonoid Q-markers in scutellaria baicalensis from different locality of growth. The flavonoid Q-markers in scutellaria baicalensis from different locality of growth were different. Suppose the total Q-marker amount of flavonoids of Hebei scutellaria baicalensis Huanglian jiedu tang was 100%,and the Shanxi scutellaria baicalensis was 66. 85%,Gansu scutellaria baicalensis was 55. 13%,Neimeng scutellaria baicalensis was 31. 16%,it suggested that origin information should add to scutellaria baicalensis quality control system.
引文
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[15]郭晓艳,郝婷,赵桂琴,等.承德产道地药材黄芩不同采收期的根中黄芩苷的含量测定[J].时珍国医国药,2014,25(4):940-942.
[16]丁舸,杨宜花.黄芩在方剂中的配伍意义[J].长春中医药大学学报,2008,24(3):266-267.
[2]李欣.黄芩种质资源的研究概况[J].世界科学技术·中医药现代化,2003,5(6):54-58.
[3]李飞.方剂学[M].北京:人民卫生出版社,2002.492.
[4]刘昌孝,陈士林,肖小河,等.中药质量标志物(Q-Marker):中药产品质量控制的新概念[J].中草药,2016,47(9):1443-1457
[5]肖小河,王伽伯,鄢丹.生物评价在中药质量标准化中的研究与应用[J].世界科学技术——中医药现代化,2014,16(3):514.
[6]刘晶晶,高晓美,张贵君,等.黄芩清热燥湿和泻火解毒药效组分分析[J].辽宁中医药大学学报,2013,15(9):90-92.
[7]崔萌萌,张贵君,徐蓓蕾,等.枯芩中清热黄酮类药效组分的测定[J].中国实验方剂学杂志,2011,17(2):83-86.
[8]张曦,李宏,候茂君,等.黄芩及其有效成分的药理学研究进展[J].天津药学,2000,12(4):8.
[9]常小平,王影.不同产地黄芩中主要有效成分含量比较[J].河南中医,2010,30(12):1176-1177.
[10]王付.经方药对研究与应用的思路与方法[J].中医药通报,2005,4(3):24-26.
[11]王俊霞,朱向东,王燕.黄芩配伍及应用研究概要[J].甘肃科技,2007,23(4):203-205.
[12]马爱团,钟秀会,孟立根,等.黄芩黄酮药理研究概况[J].中国兽医杂志,2006,42(9):39-40.
[13]苏文华,张光飞,李秀华,等.植物药材次生代谢产物的积累与环境的关系[J].中草药,2005,36(9):1415-1418.
[14]刘红宇,廖建萍.不同产地的黄芩野生药材与栽培药材质量比较[J].中国医药导报,2010,25(7):37-39.
[15]郭晓艳,郝婷,赵桂琴,等.承德产道地药材黄芩不同采收期的根中黄芩苷的含量测定[J].时珍国医国药,2014,25(4):940-942.
[16]丁舸,杨宜花.黄芩在方剂中的配伍意义[J].长春中医药大学学报,2008,24(3):266-267.