高效液相色谱法同时测定柑普茶中橙皮苷和6种儿茶素
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摘要
建立同时测定柑普茶产品中橙皮苷和6种儿茶素的高效液相色谱分析方法。样品经50%二甲基甲酰胺溶液涡旋混匀及水浴提取后,用Accucore XL C18反相色谱柱(250 mm×4.6 mm,4μm)进行分离,以乙腈–0.1%磷酸作为流动相,梯度洗脱,采用二极管阵列检测器(DAD)进行检测。在优化的实验条件下,橙皮苷和6种儿茶素的质量浓度在5～200 mg/L范围内与其色谱峰面积呈良好的线性关系,线性相关系数均为0.999 9,方法检出限为0.04～0.92 mg/kg,定量限为0.14～3.1 mg/kg,加标回收率为92.4%～99.4%,测定结果的相对标准偏差为0.20%～4.01%(n=6)。该方法简单、快速、准确,适用于柑普茶产品中橙皮苷和6种儿茶素含量的测定。
A method was developed for the simultaneous determination of hesperidin and six catechins in Ganpu tea by high performance liquid chromatography. The Ganpu tea samples were extracted with 50% dimethyl formamide solution by water bath extraction method. Chromatographic separation was performed on a waters reversed phase column of Accucore XL C18(250 mm×4.6 mm, 4μm), with gradient elution using acetonitrile–0.1% phosphoric acid solution as the mobile phase, and the detection was performed with diode array detector. Under optimum conditions, the mass concentration of hesperidin and six catechins had good linear relationship with its chromatographic peak area in the range of 5–200 mg/L with the same correlation coefficient of 0.999 9, the detection limits for 7 compounds were 0.04–0.92 mg/kg, and the quantification limits were 0.14–3.1 mg/kg. The average recoveries of 7 compounds were 92.4%–99.4%, the relative standard deviations of determination results was 0.20%–4.01%(n=6). The method is simple, rapid and accurate, it is suitable for the determination of hesperidin and six catechins in Ganpu tea products.
A method was developed for the simultaneous determination of hesperidin and six catechins in Ganpu tea by high performance liquid chromatography. The Ganpu tea samples were extracted with 50% dimethyl formamide solution by water bath extraction method. Chromatographic separation was performed on a waters reversed phase column of Accucore XL C18(250 mm×4.6 mm, 4μm), with gradient elution using acetonitrile–0.1% phosphoric acid solution as the mobile phase, and the detection was performed with diode array detector. Under optimum conditions, the mass concentration of hesperidin and six catechins had good linear relationship with its chromatographic peak area in the range of 5–200 mg/L with the same correlation coefficient of 0.999 9, the detection limits for 7 compounds were 0.04–0.92 mg/kg, and the quantification limits were 0.14–3.1 mg/kg. The average recoveries of 7 compounds were 92.4%–99.4%, the relative standard deviations of determination results was 0.20%–4.01%(n=6). The method is simple, rapid and accurate, it is suitable for the determination of hesperidin and six catechins in Ganpu tea products.
引文
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[3]宛晓春.茶叶生物化学[M].3版.北京:中国农业出版社,2003:23-40.
[4]蒋丽,王雪梅,全学军,等.不同提取方法对茶多酚理化性质的影响[J].食品科学,2010,31(14):136-139.
[5]宋冠群,林金明.茶多酚分析方法研究与最新进展[J].世界科技研究与发展,2004,26(1):41-51.
[6]国家药典委员会.中华人民共和国药典(一部)[M].北京:化学工业出版社,2005:1 321.
[7]薛芳,许占明.中国药物大全(中药卷)[M].北京:人民卫生出版社,2005.
[8]唐维,叶勇树,王国才,等.广陈皮水提物的化学成分分析[J].中国实验方剂学杂志,2015,21(4):30-33.
[9]吕海鹏,林智,谷记平,等.茶叶儿茶素组分HPLC测定中的提取方法研究[J].食品与发酵工业,2007,33(6):76-79.
[10]邹耀洪.高效液相色谱/质谱分析茶树老叶中儿茶素[J].分析化学,2003,31(3):381.
[11]李俊,郭晓关,庞宏宇,等.贵州绿茶中咖啡碱和儿茶素含量分析[J].茶叶科学,2012,32(6):480-484.
[12]郑国栋,蒋林,杨雪,等.不同贮藏年限广陈皮黄酮类成分的变化规律研究[J].中成药,2010,32(6):977-980.
[13]苏家辉,林炳国,吴声振,等.高效液相色谱法测定中药陈皮中橙皮苷的含量[J].中医临床研究,2014,6(12):131-132.
[14]吴愫青,叶莹,张俊.HPLC测定不同产地陈皮橙皮苷的含量[J].中国现代中药,2008,10(8):20-22.
[15]吴霞,叶勇树,王国才,等.HPLC法测定陈皮水提物中三种活性成分含量[J].亚太传统医药,2015,11(3):24-27.