Expression of Prominin-1 in Mouse Uterus During Peri-implantation
|
摘要
To investigate the expression of Prominin-1(Prom-1) in mouse uterus during peri-implantation. In situ hybridization and immunohistochemical staining were used to detect the mRNA and protein expression level of Prom-1 in mice uterus in early pregnancy, pseudopregnancy, estrous cycle, treated with hormone, delayed implantation and activation models. The results showed that Prom-1 was gradually weakened in uterine luminal epithelium(LE) and glandular epithelium(GE) during days 1-4 of pregnancy.During days 5-8, Prom-1 was strongly expressed in GE, and signal of Prom-1 protein was detected in matrix and decidua around the embryo. Similar to pregnancy, Prom-1 was strongly expressed in LE and GE on the 1 st day and weakly expressed on the 4 th day of pseudopregnancy. In addition, Prom-1 was highly expressed in LE and GE on estrus. Expression of Prom-1 was observed in the LE and the GE of delayed-implantation uterus. In activated-implantation animal model, Prom-1 was strongly expressed in the GE. In the hormone-treated model, Prom-1 expression levels were higher in the uterus of the 17β-estradiol-treated group than those in the control group. These results indicated that Prom-1 might promote the proliferation of mouse endometrial epithelium and participate in the establishment of uterine receptivity. Meanwhile, the expression of Prom-1 was up-regulated by the 17β-estradiol, indicating that Prom-1 might involve in the process of decidua development regulated by uterine glands, and Prom-1 might play an important role in mice early pregnancy.
To investigate the expression of Prominin-1(Prom-1) in mouse uterus during peri-implantation. In situ hybridization and immunohistochemical staining were used to detect the mRNA and protein expression level of Prom-1 in mice uterus in early pregnancy, pseudopregnancy, estrous cycle, treated with hormone, delayed implantation and activation models. The results showed that Prom-1 was gradually weakened in uterine luminal epithelium(LE) and glandular epithelium(GE) during days 1-4 of pregnancy.During days 5-8, Prom-1 was strongly expressed in GE, and signal of Prom-1 protein was detected in matrix and decidua around the embryo. Similar to pregnancy, Prom-1 was strongly expressed in LE and GE on the 1 st day and weakly expressed on the 4 th day of pseudopregnancy. In addition, Prom-1 was highly expressed in LE and GE on estrus. Expression of Prom-1 was observed in the LE and the GE of delayed-implantation uterus. In activated-implantation animal model, Prom-1 was strongly expressed in the GE. In the hormone-treated model, Prom-1 expression levels were higher in the uterus of the 17β-estradiol-treated group than those in the control group. These results indicated that Prom-1 might promote the proliferation of mouse endometrial epithelium and participate in the establishment of uterine receptivity. Meanwhile, the expression of Prom-1 was up-regulated by the 17β-estradiol, indicating that Prom-1 might involve in the process of decidua development regulated by uterine glands, and Prom-1 might play an important role in mice early pregnancy.
To investigate the expression of Prominin-1(Prom-1) in mouse uterus during peri-implantation. In situ hybridization and immunohistochemical staining were used to detect the mRNA and protein expression level of Prom-1 in mice uterus in early pregnancy, pseudopregnancy, estrous cycle, treated with hormone, delayed implantation and activation models. The results showed that Prom-1 was gradually weakened in uterine luminal epithelium(LE) and glandular epithelium(GE) during days 1-4 of pregnancy.During days 5-8, Prom-1 was strongly expressed in GE, and signal of Prom-1 protein was detected in matrix and decidua around the embryo. Similar to pregnancy, Prom-1 was strongly expressed in LE and GE on the 1 st day and weakly expressed on the 4 th day of pseudopregnancy. In addition, Prom-1 was highly expressed in LE and GE on estrus. Expression of Prom-1 was observed in the LE and the GE of delayed-implantation uterus. In activated-implantation animal model, Prom-1 was strongly expressed in the GE. In the hormone-treated model, Prom-1 expression levels were higher in the uterus of the 17β-estradiol-treated group than those in the control group. These results indicated that Prom-1 might promote the proliferation of mouse endometrial epithelium and participate in the establishment of uterine receptivity. Meanwhile, the expression of Prom-1 was up-regulated by the 17β-estradiol, indicating that Prom-1 might involve in the process of decidua development regulated by uterine glands, and Prom-1 might play an important role in mice early pregnancy.
引文
Alamgeer M,Ganju V,Szczepny A,et al.2013.The prognostic significance of aldehyde dehydrogenase 1A1(ALDH1A1)and CD133 expression in early stage non-small cell lung cancer.Thorax,68(12):1095-1104.
Boivin D,LabbéD,Fontaine N,et al.2009.The stem cell marker CD133(Prominin-1)is phosphorylated on cytoplasmic tyrosine-828 and tyrosine-852 by Src and Fyn tyrosine kinases.Biochemistry,48(18):3998-4007.
Dey S K,Lim H,Das S K,et al.2004.Molecular cues to implantation.Endocr Rev,25(3):341-73.
Dowland S N,Madawala R J,Poon C E,et al.2017.Prominin-1glycosylation changes throughout early pregnancy in uterine epithelial cells under the influence of maternal ovarian hormones.Reprod Fertil Dev,29(6):1194-1208.
Ding N Z,Wang X M,Jiao X W,et al.2018.Cellular prion protein is involved in decidualization of mouse uterus.Biol Reprod,99(2):319-325.
Glumac P M,LeBeau A M.2018.The role of CD133 in cancer:a concise review.Clin Transl Med,7(1):18.
Hou X,Tan Y,Li M,et al.2004.Canonical Wnt signaling is critical to estrogen-mediated uterine growth.Mol Endocrinol,18(12):3035-3049.
Huang M,Zhu H,Feng J,et al.2015.High CD133 expression in the nucleus and cytoplasm predicts poor prognosis in non-small cell lung cancer.Dis Markers,2015:986095.
Jang H.2018.Regulation of cyclic AMP-response element binding protein zhangfei(CREBZF)expression by estrogen in mouse uterus.Dev Reprod,22(1):95-104.
KarbanováJ,Missol-Kolka E,Fonseca A V,et al.2008.The stem cell marker CD133(Prominin-1)is expressed in various human glandular epithelia.J Histochem Cytochem,56(11):977-93.
Kelleher A M,Milano-Foster J,Behura S K,et al.2018.Uterine glands coordinate on-time embryo implantation and impact endometrial decidualization for pregnancy success.Nat Commun,9(1):2435.
Li Z.2013.CD133:a stem cell biomarker and beyond.Exp Hematol Oncol,2(1):17.
Liu S,Zhou J,Wen J.2015.Expression and significance of CD133 and ABCG2 in endometriosis.Clin Exp Obstet Gynecol,42(6):771-775.
Miraglia S,Godfrey W,Yin A H,et al.1997.A novel five-transmembrane hematopoietic stem cell antigen:isolation,characterization,and molecular cloning.Blood,90(12):5013-5021.
Mak A B,Nixon A M,Kittanakom S,et al.2012.Regulation of CD133 by HDAC6 promotesβ-catenin signaling to suppress cancer cell differentiation.Cell Rep,2(4):951-63.
Nomura A,Banerjee S,Chugh R,et al.2015.CD133 initiates tumors,induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer.Oncotarget,6(10):8313-8322.
Shi C,Tian R,Wang M,et al.2010.CD44+CD133+population exhibits cancer stem cell-like characteristics in human gallbladder carcinoma.Cancer Biol Ther,10(11):1182-1190.
Tepekoy F,Akkoyunlu G,Demir R.2015.The role of Wnt signaling members in the uterus and embryo during pre-implantation and implantation.J Assist Reprod Genet,32(3):337-346.
Wang H B.2017.Embryo implantation:progress and challenge.Chinese Bulletin of Life Sciences,1:31-42.
Wakamatsu Y,Sakamoto N,Oo H Z,et al.2012.Expression of cancer stem cell markers ALDH1,CD44 and CD133 in primary tumor and lymph node metastasis of gastric cancer.Pathol Int,62(2):112-119.
Wei Y,Jiang Y,Zou F,et al.2013.Activation of PI3K/Akt pathway by CD133-p85 interaction promotes tumorigenic capacity of glioma stem cells.Proc Natl Acad Sci USA,110(17):6829-6934.
Weigmann A,Corbeil D,Hellwig A,et al.1997.Prominin,a novel microvilli-specific polytopic membrane protein of the apical surface of epithelial cells,is targeted to plasmalemmal protrusions of nonepithelial cells.Proc Natl Acad Sci USA,94(23):12425-12430.
Wu H,Qi X W,Yan G N,et al.2014.Is CD133 expression a prognostic biomarker of non-small-cell lung cancer?A systematic review and meta-analysis.PLoS One,9(6):e100168.
Yuan J,Deng W,Cha J,et al.2018.Tridimensional visualization reveals direct communication between the embryo and glands critical for implantation.Nat Commun,9(1):603.
Zhu L,Pollard J W.2007.Estradiol-17beta regulates mouse uterine epithelial cell proliferation through insulin-like growth factor 1signaling.Proc Natl Acad Sci USA,104(40):15847-15851.
Boivin D,LabbéD,Fontaine N,et al.2009.The stem cell marker CD133(Prominin-1)is phosphorylated on cytoplasmic tyrosine-828 and tyrosine-852 by Src and Fyn tyrosine kinases.Biochemistry,48(18):3998-4007.
Dey S K,Lim H,Das S K,et al.2004.Molecular cues to implantation.Endocr Rev,25(3):341-73.
Dowland S N,Madawala R J,Poon C E,et al.2017.Prominin-1glycosylation changes throughout early pregnancy in uterine epithelial cells under the influence of maternal ovarian hormones.Reprod Fertil Dev,29(6):1194-1208.
Ding N Z,Wang X M,Jiao X W,et al.2018.Cellular prion protein is involved in decidualization of mouse uterus.Biol Reprod,99(2):319-325.
Glumac P M,LeBeau A M.2018.The role of CD133 in cancer:a concise review.Clin Transl Med,7(1):18.
Hou X,Tan Y,Li M,et al.2004.Canonical Wnt signaling is critical to estrogen-mediated uterine growth.Mol Endocrinol,18(12):3035-3049.
Huang M,Zhu H,Feng J,et al.2015.High CD133 expression in the nucleus and cytoplasm predicts poor prognosis in non-small cell lung cancer.Dis Markers,2015:986095.
Jang H.2018.Regulation of cyclic AMP-response element binding protein zhangfei(CREBZF)expression by estrogen in mouse uterus.Dev Reprod,22(1):95-104.
KarbanováJ,Missol-Kolka E,Fonseca A V,et al.2008.The stem cell marker CD133(Prominin-1)is expressed in various human glandular epithelia.J Histochem Cytochem,56(11):977-93.
Kelleher A M,Milano-Foster J,Behura S K,et al.2018.Uterine glands coordinate on-time embryo implantation and impact endometrial decidualization for pregnancy success.Nat Commun,9(1):2435.
Li Z.2013.CD133:a stem cell biomarker and beyond.Exp Hematol Oncol,2(1):17.
Liu S,Zhou J,Wen J.2015.Expression and significance of CD133 and ABCG2 in endometriosis.Clin Exp Obstet Gynecol,42(6):771-775.
Miraglia S,Godfrey W,Yin A H,et al.1997.A novel five-transmembrane hematopoietic stem cell antigen:isolation,characterization,and molecular cloning.Blood,90(12):5013-5021.
Mak A B,Nixon A M,Kittanakom S,et al.2012.Regulation of CD133 by HDAC6 promotesβ-catenin signaling to suppress cancer cell differentiation.Cell Rep,2(4):951-63.
Nomura A,Banerjee S,Chugh R,et al.2015.CD133 initiates tumors,induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer.Oncotarget,6(10):8313-8322.
Shi C,Tian R,Wang M,et al.2010.CD44+CD133+population exhibits cancer stem cell-like characteristics in human gallbladder carcinoma.Cancer Biol Ther,10(11):1182-1190.
Tepekoy F,Akkoyunlu G,Demir R.2015.The role of Wnt signaling members in the uterus and embryo during pre-implantation and implantation.J Assist Reprod Genet,32(3):337-346.
Wang H B.2017.Embryo implantation:progress and challenge.Chinese Bulletin of Life Sciences,1:31-42.
Wakamatsu Y,Sakamoto N,Oo H Z,et al.2012.Expression of cancer stem cell markers ALDH1,CD44 and CD133 in primary tumor and lymph node metastasis of gastric cancer.Pathol Int,62(2):112-119.
Wei Y,Jiang Y,Zou F,et al.2013.Activation of PI3K/Akt pathway by CD133-p85 interaction promotes tumorigenic capacity of glioma stem cells.Proc Natl Acad Sci USA,110(17):6829-6934.
Weigmann A,Corbeil D,Hellwig A,et al.1997.Prominin,a novel microvilli-specific polytopic membrane protein of the apical surface of epithelial cells,is targeted to plasmalemmal protrusions of nonepithelial cells.Proc Natl Acad Sci USA,94(23):12425-12430.
Wu H,Qi X W,Yan G N,et al.2014.Is CD133 expression a prognostic biomarker of non-small-cell lung cancer?A systematic review and meta-analysis.PLoS One,9(6):e100168.
Yuan J,Deng W,Cha J,et al.2018.Tridimensional visualization reveals direct communication between the embryo and glands critical for implantation.Nat Commun,9(1):603.
Zhu L,Pollard J W.2007.Estradiol-17beta regulates mouse uterine epithelial cell proliferation through insulin-like growth factor 1signaling.Proc Natl Acad Sci USA,104(40):15847-15851.