鼻咽癌组织中PTTG和cyclinD1蛋白的表达及临床意义
|
摘要
目的探讨鼻咽癌组织中垂体肿瘤转化基因(PTTG)和细胞周期素D1(cyclinD1)蛋白的表达及其临床意义。方法采用免疫组织化学检测PTTG和cyclinD1蛋白在68例鼻咽癌、22例癌前病变及25例鼻咽黏膜慢性炎组织中的表达情况。结果 PTTG和cyclinD1蛋白在鼻咽黏膜慢性炎组织中的阳性表达率分别为4.0%和8.0%,癌前病变为40.9%和63.6%,鼻咽癌组织为80.9%和72.1%,PTTG蛋白的表达在鼻咽癌与癌前病变和鼻咽黏膜慢性炎组织中比较,差异均有统计学意义(P<0.05);鼻咽癌与癌前病变组织比较,差异有统计学意义(P<0.05);PTTG蛋白的高表达与鼻咽癌的临床分期和淋巴结转移有关(P<0.05)。cyclinD1蛋白的表达在鼻咽癌与鼻咽黏膜慢性炎组织中比较,差异有统计学意义(P<0.05),而在鼻咽癌与癌前病变中比较,差异无统计学意义(P>0.05),cyclinD1蛋白的高表达与临床分期和淋巴结转移无关;PTTG与cyclinD1表达两者无相关性(P>0.05)。结论 PTTG和cyclinD1蛋白的高表达参与了鼻咽癌的发生、发展过程,PTTG基因的突变或激活可能还与鼻咽癌的预后有关。
Objective To detect the expressions of pituitary tumor transforming gene(PTTG)and cyclinD1 protein in nasopharyngeal carcinoma,and to elucidate their clinical significance.Methods We detected the expressions of PTTG and cyclinD1 protein in 68 cases of nasopharyngeal carcinoma,22 cases of precancerous lesion and 25 cases of inflammatory nasopharyngeal mucosa by immunohistochemistry method.Results The expression rates of PTTG protein were 4.0%in inflammatory nasopharyngeal mucosa,40.9%in precancerous lesion and 80.9%in nasopharyngeal carcinoma,which were significantly different from that in inflammatory nasopharyngeal mucosa by statistic analysis(P<0.05),the expression rate in nasopharyngeal carcinoma was also significantly different from that in precancerous lesion by statistic analysis(P<0.05).The higher expression of PTTG protein was correlated with clinical stage and lymph node metastasis of nasopharyngeal carcinoma.The expression rates of cyclinD1 protein were 8.0% in inflammatory nasopharyngeal mucosa,63.6%in precancerous lesion and 72.1%in nasopharyngeal carcinoma,which were significantly different from that in inflammatory nasopharyngeal mucosa by statistic analysis(P<0.05),but the expression rate in nasopharyngeal carcinoma was not significantly different from that in precancerous lesion by statistic analysis(P>0.05).The higher expression of cyclinD1 protein was not related with clinical stage and lymph node metastasis of nasopharyngeal carcinoma.Conclusion The higher expression of PTTG and cyclinD1 protein might participate in nasopharyngeal carcinogenesis.Mutation and stimulation of PTTG gene might be associated to the prognosis of nasopharyngeal carcinoma.
Objective To detect the expressions of pituitary tumor transforming gene(PTTG)and cyclinD1 protein in nasopharyngeal carcinoma,and to elucidate their clinical significance.Methods We detected the expressions of PTTG and cyclinD1 protein in 68 cases of nasopharyngeal carcinoma,22 cases of precancerous lesion and 25 cases of inflammatory nasopharyngeal mucosa by immunohistochemistry method.Results The expression rates of PTTG protein were 4.0%in inflammatory nasopharyngeal mucosa,40.9%in precancerous lesion and 80.9%in nasopharyngeal carcinoma,which were significantly different from that in inflammatory nasopharyngeal mucosa by statistic analysis(P<0.05),the expression rate in nasopharyngeal carcinoma was also significantly different from that in precancerous lesion by statistic analysis(P<0.05).The higher expression of PTTG protein was correlated with clinical stage and lymph node metastasis of nasopharyngeal carcinoma.The expression rates of cyclinD1 protein were 8.0% in inflammatory nasopharyngeal mucosa,63.6%in precancerous lesion and 72.1%in nasopharyngeal carcinoma,which were significantly different from that in inflammatory nasopharyngeal mucosa by statistic analysis(P<0.05),but the expression rate in nasopharyngeal carcinoma was not significantly different from that in precancerous lesion by statistic analysis(P>0.05).The higher expression of cyclinD1 protein was not related with clinical stage and lymph node metastasis of nasopharyngeal carcinoma.Conclusion The higher expression of PTTG and cyclinD1 protein might participate in nasopharyngeal carcinogenesis.Mutation and stimulation of PTTG gene might be associated to the prognosis of nasopharyngeal carcinoma.
引文
[1]HAMID T,KAKAR S S.PTTG and cancer[J].Histol Histopathol,2003,18(1):245-251.
[2]ZHANG X,HORWITZ G A,PREZANT T R,et al.Structure,expression,and function of human pituitary tumor-transforming gene(PTTG)[J].Mol Endocrinol,1999,13(1):156-166.
[3]王先丽,莫维光.PTTG基因的结构、功能及其与肿瘤的关系[J].医学文选,2006,25(1):128-130.
[4]HUI A B,LO K W,LEUNG S F,et al.Detection of recurrent chromosomal gains and lossesin primary nasop haryngeal carcinoma by comparative genomic hybridization[J].Int J Cancer,1999,82(4):498-503.
[5]厉浩,刘保安.鼻咽癌中PTTG的表达及其临床病理意义[J].湖南师范大学学报(医学版),2008,5(2):10-13.
[6]UMEKITA Y,OHI Y,SAGARA Y,et al.Overexpression of cyclinD1predicts for poor prognosis in estrogen receptor-negative breast cancer patients[J].Int J Cancer,2002,98(3):415-418.
[7]梁朝晖,胡少为,何青莲.P16和cyclin D1在鼻咽癌及癌前病变中表达的临床意义[J].国际医药卫生导报,2009,15(12):18-21.
[8]钟守军,姚运红,李飞虹,等.EGR-1与p53,p16,CyclinD1在鼻咽癌中的表达及意义[J].肿瘤研究与临床,2006,18(10):664-666.
[2]ZHANG X,HORWITZ G A,PREZANT T R,et al.Structure,expression,and function of human pituitary tumor-transforming gene(PTTG)[J].Mol Endocrinol,1999,13(1):156-166.
[3]王先丽,莫维光.PTTG基因的结构、功能及其与肿瘤的关系[J].医学文选,2006,25(1):128-130.
[4]HUI A B,LO K W,LEUNG S F,et al.Detection of recurrent chromosomal gains and lossesin primary nasop haryngeal carcinoma by comparative genomic hybridization[J].Int J Cancer,1999,82(4):498-503.
[5]厉浩,刘保安.鼻咽癌中PTTG的表达及其临床病理意义[J].湖南师范大学学报(医学版),2008,5(2):10-13.
[6]UMEKITA Y,OHI Y,SAGARA Y,et al.Overexpression of cyclinD1predicts for poor prognosis in estrogen receptor-negative breast cancer patients[J].Int J Cancer,2002,98(3):415-418.
[7]梁朝晖,胡少为,何青莲.P16和cyclin D1在鼻咽癌及癌前病变中表达的临床意义[J].国际医药卫生导报,2009,15(12):18-21.
[8]钟守军,姚运红,李飞虹,等.EGR-1与p53,p16,CyclinD1在鼻咽癌中的表达及意义[J].肿瘤研究与临床,2006,18(10):664-666.