响应面优化超声辅助提取鸡桑叶多糖工艺
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摘要
为研究超声辅助提取鸡桑叶多糖的工艺,以超声功率、超声时间、超声温度和液料比为工艺指标,研究不同工艺参数对鸡桑叶多糖提取率的影响,并用响应面对超声辅助提取鸡桑叶多糖工艺进行优化。结果表明,鸡桑叶多糖的最佳提取工艺为:超声功率80 W、超声时间26 min、超声温度73℃和液料比19∶1(m L/g),在此条件下,鸡桑叶多糖提取率为48.21 mg/g,与预测值相比,其相对误差为0.41%。
In order to research the ultrasonic-assisted extraction of polysaccharides from Morus australis leaves,the influence of process parameters including ultrasonic power,ultrasonic time,temperature and liquid-solid ratio on polysaccharides yield from Morus australis leaves were investigated and the response surface methodology was used to optimize. Results showed that the optimal process of ultrasound-assisted extraction of polysaccharides from Morus australis leaves was ultrasonic power 80 W,time 26 min,temperature73 ℃ and liquid-solid ratio 19 ∶ 1(m L/g). Under these conditions,the polysaccharide extraction yield was48.21 mg/g,the relative error was 0.41 % compared with the predicted value.
In order to research the ultrasonic-assisted extraction of polysaccharides from Morus australis leaves,the influence of process parameters including ultrasonic power,ultrasonic time,temperature and liquid-solid ratio on polysaccharides yield from Morus australis leaves were investigated and the response surface methodology was used to optimize. Results showed that the optimal process of ultrasound-assisted extraction of polysaccharides from Morus australis leaves was ultrasonic power 80 W,time 26 min,temperature73 ℃ and liquid-solid ratio 19 ∶ 1(m L/g). Under these conditions,the polysaccharide extraction yield was48.21 mg/g,the relative error was 0.41 % compared with the predicted value.
引文
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[2]Ko H H,Wang J J,Lin H C,et al.Chemistry and biological activities of constituents from Morus australis[J].Biochimica et Biophysica Acta(BBA)-General Subjects,1999,1428(2):293-299
[3]Takahashi M,Takara K,Toyozato T,et al.A novel bioactive chalcone of Morus australis inhibits tyrosinase activity and melanin biosynthesis in B16 melanoma cells[J].Journal of oleo science,2012,61(10):585-592
[4]Wang J,Lu H D,Muhammad U,et al.Ultrasound-assisted extraction of polysaccharides from Artemisia selengensis Turcz and its antioxidant and anticancer activities[J].Journal of food science and technology,2016,53(2):1025-1034
[5]Zhu W,Xue X,Zhang Z.Ultrasonic-assisted extraction,structure and antitumor activity of polysaccharide from Polygonum multiflorum[J].International journal of biological macromolecules,2016,91(10):132-142
[6]陈文娟,陈建福.血柚皮多糖的碱法提取及其抗氧化活性研究[J].中央民族大学学报(自然科学版),2014,23(3):22-27
[7]Li L,Li X,Ding C,et al.Ultrasonic-assisted enzymatic extraction and antioxidant activity of polysaccharides from Setaria viridis[J].Separation Science and Technology,2016,51(11):1798-1805
[8]Aguilo-Aguayo I,Walton J,Vinas I,et al.Ultrasound assisted extraction of polysaccharides from mushroom by-products[J].LWTFood Science and Technology,2017,77(4):92-99
[9]陈文娟,陈建福,吴丽敏,等.响应面优化超声辅助提取扫帚菜多糖工艺及其抗氧化性能[J].食品工业科技,2017,38(11):219-223
[10]Raza A,Li F,Xu X,et al.Optimization of ultrasonic-assisted extraction of antioxidant polysaccharides from the stem of Trapa quadrispinosa using response surface methodology[J].International journal of biological macromolecules,2017,94(1):335-344