微生物检验技术研究进展
|
摘要
食源性致病菌是导致疾病发生的重要原因,严重威胁着人类的健康。加强对食品中微生物的检验可以有效的遏制社会公共卫生问题的发生,夯实微生物检测技术,不断创新检测技术。从微生物检测技术应用与比较出发,对微滴数字聚合酶链式反应技术、叠氮溴化乙锭-聚合酶链式反应、可视化基因芯片技术和荧光标记噬菌体等近年来出现的新型检测技术进行论述。
Foodborne pathogenic bacteria are an important cause of disease and seriously threaten human health.Strengthening the inspection of microorganism in food can effectively curb the occurrence of social public health problems,consolidate the detection technology of microorganism,and constantly innovate the detection technology.Starting from the microbial detection technology application and comparison,the droplet digital polymerase chain reaction(PCR)technique,ethidium monoazide bromide-polymerase chain reaction(EMA-PCR),visualize,gene chip technology and fluorescent tags phage,in recent years the emergence of new detection technology was discussed.
Foodborne pathogenic bacteria are an important cause of disease and seriously threaten human health.Strengthening the inspection of microorganism in food can effectively curb the occurrence of social public health problems,consolidate the detection technology of microorganism,and constantly innovate the detection technology.Starting from the microbial detection technology application and comparison,the droplet digital polymerase chain reaction(PCR)technique,ethidium monoazide bromide-polymerase chain reaction(EMA-PCR),visualize,gene chip technology and fluorescent tags phage,in recent years the emergence of new detection technology was discussed.
引文
[1]孙凤霞.探析食品微生物检验技术的研究进展[J].中国医药指南,2014,12(23):69-70
[2]高晓平,韩颖,黄现青.食品病原微生物分子检测技术研究进展[J].粮食与油脂,2008(9):47-48
[3]王国琴.食品微生物之水产品中的微生物检验方法研究进展[J].中国现代医生,2013,51(7):116-117
[4]梁义梅.微生物的检验技术与未来发展探讨[J].医学信息,2015(50):159-160
[5]Liu-Stratton Y,Roy S,Sen C K.DNA microarray technology in nutraceutical and food safety[J].Toxicology Letters,2004,150(1):29-42
[6]Ostroff R M,Hopkins D,Haeberli A B,et al.Thin film biosensor for rapid visual detection of nucleic acid targets[J].Clinical Chemistry,1999,45(9):1659-1664
[7]年梦婷.食品微生物检测技术应用现状及展望[J].食品界,2017(6):94
[8]张冲,刘祥,陈计峦.食品中微生物检测新技术研究进展[J].食品研究与开发,2011,32(12):212-216
[9]Mosier-Boss P A,Lieberman S H,Andrews J M,et al.Use of fluorescently labeled phage in the detection and identification of bacterial species[J].Applied Spectroscopy,2003,57(9):1138-1144
[10]Phea M H,Dossotb M,Guilloteau H,et al.Nucleic acid fluorochromes and flow cytometry prove useful in assessing the effect of chlorination on drinking water bacteria[J].Water research,2005,39(15):3618-3628
[11]Flajshans M,Cossonc J,Redina M.The application of image cytometry to viability assessment in dual fluorescence-stained fish spermatozoa[J].Cell biol intemational,2004,28(12):955-959
[12]罗荣,任秀,崔生辉.食品中沙门氏菌快速检测技术研究进展[J].食品安全质量检测学报,2016,7(4):1468-1472
[13]Cheng CM.Rapid detection of Salmonella in foods using real time PCR[J].J Food Prot,2008,71(12):2436-2441
[14]Bialek H,Dawes J,Heer D,et al.Portable real-time PCR system using tablet-based fluorescence imaging[C].IEEE:Student Conference,2016:1-4
[15]李平兰.PCR技术及其在食品微生物检测中的应用[J].食品科学,1998,19(7):3-5
[16]常玉华,仇农学.基于PCR技术快速检测食品微生物的原理方法与应用[J].农产品加工·学刊,2011(11):4-6
[17]刘赛.生物技术在食品检测中的应用[J].食品安全导刊,2017(3):65
[18]Azuka Iwobi,Lars Gerdes,Ulrich Busch,et al.Droplet digital PCR for routine analysis of genetically modified foods(GMO)-A comparison with real-time quantitative PCR[J].Food Control,2016,69:205-213
[19]George D,Czech J,John B,et al.Detection and quantification of chimerism by droplet digital PCR[J].Chimerism,2013,4(3):102-108
[20]Beck J,Bierau S,Balzer S,et al.Digital droplet PCR for rapid quantification of donor DNA in the circulation of transplant recipients as a potential universal biomarker of graft injury[J].Clinical Chemistry,2013,59(12):1732-1741
[21]祝儒刚,吕淑霞,刘月萍,等.基于DNA染料EMA的PCR技术检测鉴别副溶血性弧菌死活细胞[J].食品与发酵工业,2010(7):144-149
[22]吕嫱,姜兆兴,王海艳,等.叠氮溴化乙锭与PCR技术结合检测活菌研究进展[J].食品研究与开发,2017,38(24):220-224
[2]高晓平,韩颖,黄现青.食品病原微生物分子检测技术研究进展[J].粮食与油脂,2008(9):47-48
[3]王国琴.食品微生物之水产品中的微生物检验方法研究进展[J].中国现代医生,2013,51(7):116-117
[4]梁义梅.微生物的检验技术与未来发展探讨[J].医学信息,2015(50):159-160
[5]Liu-Stratton Y,Roy S,Sen C K.DNA microarray technology in nutraceutical and food safety[J].Toxicology Letters,2004,150(1):29-42
[6]Ostroff R M,Hopkins D,Haeberli A B,et al.Thin film biosensor for rapid visual detection of nucleic acid targets[J].Clinical Chemistry,1999,45(9):1659-1664
[7]年梦婷.食品微生物检测技术应用现状及展望[J].食品界,2017(6):94
[8]张冲,刘祥,陈计峦.食品中微生物检测新技术研究进展[J].食品研究与开发,2011,32(12):212-216
[9]Mosier-Boss P A,Lieberman S H,Andrews J M,et al.Use of fluorescently labeled phage in the detection and identification of bacterial species[J].Applied Spectroscopy,2003,57(9):1138-1144
[10]Phea M H,Dossotb M,Guilloteau H,et al.Nucleic acid fluorochromes and flow cytometry prove useful in assessing the effect of chlorination on drinking water bacteria[J].Water research,2005,39(15):3618-3628
[11]Flajshans M,Cossonc J,Redina M.The application of image cytometry to viability assessment in dual fluorescence-stained fish spermatozoa[J].Cell biol intemational,2004,28(12):955-959
[12]罗荣,任秀,崔生辉.食品中沙门氏菌快速检测技术研究进展[J].食品安全质量检测学报,2016,7(4):1468-1472
[13]Cheng CM.Rapid detection of Salmonella in foods using real time PCR[J].J Food Prot,2008,71(12):2436-2441
[14]Bialek H,Dawes J,Heer D,et al.Portable real-time PCR system using tablet-based fluorescence imaging[C].IEEE:Student Conference,2016:1-4
[15]李平兰.PCR技术及其在食品微生物检测中的应用[J].食品科学,1998,19(7):3-5
[16]常玉华,仇农学.基于PCR技术快速检测食品微生物的原理方法与应用[J].农产品加工·学刊,2011(11):4-6
[17]刘赛.生物技术在食品检测中的应用[J].食品安全导刊,2017(3):65
[18]Azuka Iwobi,Lars Gerdes,Ulrich Busch,et al.Droplet digital PCR for routine analysis of genetically modified foods(GMO)-A comparison with real-time quantitative PCR[J].Food Control,2016,69:205-213
[19]George D,Czech J,John B,et al.Detection and quantification of chimerism by droplet digital PCR[J].Chimerism,2013,4(3):102-108
[20]Beck J,Bierau S,Balzer S,et al.Digital droplet PCR for rapid quantification of donor DNA in the circulation of transplant recipients as a potential universal biomarker of graft injury[J].Clinical Chemistry,2013,59(12):1732-1741
[21]祝儒刚,吕淑霞,刘月萍,等.基于DNA染料EMA的PCR技术检测鉴别副溶血性弧菌死活细胞[J].食品与发酵工业,2010(7):144-149
[22]吕嫱,姜兆兴,王海艳,等.叠氮溴化乙锭与PCR技术结合检测活菌研究进展[J].食品研究与开发,2017,38(24):220-224